HEC3_ARATH
ID HEC3_ARATH Reviewed; 224 AA.
AC Q9LXD8;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription factor HEC3;
DE AltName: Full=Basic helix-loop-helix protein 43;
DE Short=AtbHLH43;
DE Short=bHLH 43;
DE AltName: Full=Protein HECATE 3;
DE AltName: Full=Transcription factor EN 119;
DE AltName: Full=bHLH transcription factor bHLH043;
GN Name=HEC3; Synonyms=BHLH43, EN119; OrderedLocusNames=At5g09750;
GN ORFNames=F17I14.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP FUNCTION, INTERACTION WITH SPT, INDUCTION BY ETT, DEVELOPMENTAL STAGE,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17855426; DOI=10.1242/dev.011510;
RA Gremski K., Ditta G., Yanofsky M.F.;
RT "The HECATE genes regulate female reproductive tract development in
RT Arabidopsis thaliana.";
RL Development 134:3593-3601(2007).
CC -!- FUNCTION: Required for the female reproductive tract development and
CC fertility. {ECO:0000269|PubMed:17855426}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with SPT.
CC {ECO:0000269|PubMed:17855426, ECO:0000305}.
CC -!- INTERACTION:
CC Q9LXD8; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-1536734, EBI-15191535;
CC Q9LXD8; Q5XVH0: BHLH109; NbExp=5; IntAct=EBI-1536734, EBI-15193531;
CC Q9LXD8; Q9LV17: BHLH79; NbExp=3; IntAct=EBI-1536734, EBI-15191993;
CC Q9LXD8; O82277: TCP10; NbExp=3; IntAct=EBI-1536734, EBI-3133327;
CC Q9LXD8; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-1536734, EBI-4426144;
CC Q9LXD8; Q8LPR5: TCP4; NbExp=3; IntAct=EBI-1536734, EBI-15192325;
CC Q9LXD8; Q9FME3: TCP5; NbExp=3; IntAct=EBI-1536734, EBI-15192251;
CC Q9LXD8; O64647: TCP9; NbExp=3; IntAct=EBI-1536734, EBI-9838721;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Gynoecium. {ECO:0000269|PubMed:17855426}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing septum, transmitting
CC tract and stigma. {ECO:0000269|PubMed:17855426}.
CC -!- INDUCTION: Negatively regulated by ARF3/ETT in the abaxial gynoecium.
CC {ECO:0000269|PubMed:17855426}.
CC -!- DISRUPTION PHENOTYPE: Impaired pollen tube growth.
CC {ECO:0000269|PubMed:17855426}.
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DR EMBL; AB020752; BAB09533.1; -; Genomic_DNA.
DR EMBL; AL353994; CAB89355.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91442.1; -; Genomic_DNA.
DR PIR; T49923; T49923.
DR RefSeq; NP_196537.1; NM_121012.2.
DR AlphaFoldDB; Q9LXD8; -.
DR SMR; Q9LXD8; -.
DR BioGRID; 16113; 61.
DR IntAct; Q9LXD8; 57.
DR STRING; 3702.AT5G09750.1; -.
DR PaxDb; Q9LXD8; -.
DR PRIDE; Q9LXD8; -.
DR EnsemblPlants; AT5G09750.1; AT5G09750.1; AT5G09750.
DR GeneID; 830835; -.
DR Gramene; AT5G09750.1; AT5G09750.1; AT5G09750.
DR KEGG; ath:AT5G09750; -.
DR Araport; AT5G09750; -.
DR TAIR; locus:2144791; AT5G09750.
DR eggNOG; ENOG502RZ93; Eukaryota.
DR HOGENOM; CLU_108253_0_0_1; -.
DR InParanoid; Q9LXD8; -.
DR OMA; TWNNIIM; -.
DR OrthoDB; 1511639at2759; -.
DR PRO; PR:Q9LXD8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LXD8; baseline.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0048462; P:carpel formation; IMP:TAIR.
DR GO; GO:0048467; P:gynoecium development; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0010500; P:transmitting tissue development; IGI:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR045843; IND-like.
DR PANTHER; PTHR45914; PTHR45914; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..224
FT /note="Transcription factor HEC3"
FT /id="PRO_0000271282"
FT DOMAIN 125..174
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 22..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 224 AA; 25400 MW; 01CB13750D7BF06F CRC64;
MNNYNMNPSL FQNYTWNNII NSSNNNNKND DHHHQHNNDP IGMAMDQYTQ LHIFNPFSSS
HFPPLSSSLT TTTLLSGDQE DDEDEEEPLE ELGAMKEMMY KIAAMQSVDI DPATVKKPKR
RNVRISDDPQ SVAARHRRER ISERIRILQR LVPGGTKMDT ASMLDEAIRY VKFLKRQIRL
LNNNTGYTPP PPQDQASQAV TTSWVSPPPP PSFGRGGRGV GELI
