ID   3SAW_NAJNA              Reviewed;          10 AA.
AC   B3EWR3;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   03-AUG-2022, entry version 15.
DE   RecName: Full=Cytotoxin NN-32;
DE   Flags: Fragment;
OS   Naja naja (Indian cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=35670;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TOXIC DOSE, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=21530568; DOI=10.1016/j.toxicon.2011.04.012;
RA   Das T., Bhattacharya S., Halder B., Biswas A., Das gupta S., Gomes A.,
RA   Gomes A.;
RT   "Cytotoxic and antioxidant property of a purified fraction (NN-32) of
RT   Indian Naja naja venom on Ehrlich ascites carcinoma in BALB/c mice.";
RL   Toxicon 57:1065-1072(2011).
CC   -!- FUNCTION: Shows cytotoxic activity against Ehrlich ascites carcinoma
CC       (EAC) model in mice. Also shows effect on apoptotic features such as
CC       nuclear fragmentation, caspase activity, pro- and anti-apoptotic
CC       protein levels on treated EAC cells. Increased levels of antioxidant
CC       enzymes and cytokine levels observed in toxin-treated EAC mice.
CC       Cardiotoxic towards guinea pig auricle. Has no cytotoxicity towards
CC       macrophages in vivo in EAC bearing mice or in vitro in normal human
CC       lymphocytes. {ECO:0000269|PubMed:21530568}.
CC   -!- SUBUNIT: Monomer in solution; Homodimer and oligomer in the presence of
CC       negatively charged lipids forming a pore with a size ranging between 20
CC       and 30 Angstroms. {ECO:0000250|UniProtKB:P60301}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21530568}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P60301}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6758; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21530568};
CC   -!- TOXIC DOSE: LD(50) is 4 mg/kg by intraperitoneal injection in mice.
CC       {ECO:0000269|PubMed:21530568}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type IA cytotoxin sub-subfamily. {ECO:0000305}.
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DR   Proteomes; UP000694559; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cardiotoxin; Cytolysis; Direct protein sequencing; Membrane;
KW   Reference proteome; Secreted; Target cell membrane; Target membrane; Toxin.
FT   PEPTIDE         1..>10
FT                   /note="Cytotoxin NN-32"
FT                   /evidence="ECO:0000269|PubMed:21530568"
FT                   /id="PRO_0000420493"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|PubMed:21530568"
SQ   SEQUENCE   10 AA;  1175 MW;  9E3E4B72C723344E CRC64;
     LKCNKLVPLF