HEI10_ORYSJ
ID HEI10_ORYSJ Reviewed; 303 AA.
AC I6PL68; A0A0P0VGR2; B9F4F5; I6PII6; Q6EUG3;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 2.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=E3 ubiquitin-protein ligase CCNB1IP1 homolog {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000305};
DE AltName: Full=RING finger-containing protein HEI10 {ECO:0000303|PubMed:22792078};
DE Short=OsHEI10 {ECO:0000303|PubMed:31266799};
DE AltName: Full=RING-type E3 ubiquitin transferase HEI10 {ECO:0000305};
GN Name=HEI10 {ECO:0000303|PubMed:22792078};
GN OrderedLocusNames=Os02g0232100 {ECO:0000312|EMBL:BAS77787.1},
GN LOC_Os02g13810 {ECO:0000305};
GN ORFNames=OJ1705_E12.17 {ECO:0000312|EMBL:BAD27706.1},
GN OsJ_05990 {ECO:0000312|EMBL:EEE56612.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, DISRUPTION
RP PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare, and cv. Wuxiangjing 9;
RX PubMed=22792078; DOI=10.1371/journal.pgen.1002809;
RA Wang K., Wang M., Tang D., Shen Y., Miao C., Hu Q., Lu T., Cheng Z.;
RT "The role of rice HEI10 in the formation of meiotic crossovers.";
RL PLoS Genet. 8:E1002809-E1002809(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP INTERACTION WITH ZIP4 AND PTD.
RX PubMed=31266799; DOI=10.1104/pp.19.00082;
RA Zhang J., Wang C., Higgins J.D., Kim Y.J., Moon S., Jung K.H., Qu S.,
RA Liang W.;
RT "A multiprotein complex regulates interference-sensitive crossover
RT formation in rice.";
RL Plant Physiol. 181:221-235(2019).
CC -!- FUNCTION: Ubiquitin E3 ligase required for class I crossover (CO)
CC formation during meiosis. {ECO:0000269|PubMed:22792078}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: Interacts with ZIP4 and PTD. {ECO:0000269|PubMed:31266799}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22792078}. Chromosome
CC {ECO:0000269|PubMed:22792078}. Note=Dynamic localization on the meiotic
CC chromosomes. Initially appears as distinct foci. Later observed along
CC the chromosomes and finally restrict to prominent foci that specially
CC localize to chiasma sites. Extends along the chromosome in the wake of
CC synapsis. {ECO:0000269|PubMed:22792078}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=HEI10A;
CC IsoId=I6PL68-1; Sequence=Displayed;
CC Name=2; Synonyms=HEI10B;
CC IsoId=I6PL68-2; Sequence=VSP_053850;
CC -!- TISSUE SPECIFICITY: Expressed in young panicles.
CC {ECO:0000269|PubMed:22792078}.
CC -!- DISRUPTION PHENOTYPE: The mutants hei10-1 and hei10-2 have normal
CC vegetative growth but exhibited complete sterility, due to shrunken and
CC inviable pollen as well as sterile female gametes. Reduced chiasma
CC frequency, with a random distribution among cells of remaining
CC chiasmata, but normal early recombination events and synaptonemal
CC complex (SC) formation. {ECO:0000269|PubMed:22792078}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEE56612.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; JQ624877; AFJ04809.1; -; mRNA.
DR EMBL; JQ624878; AFJ04810.1; -; mRNA.
DR EMBL; AP004070; BAD27706.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08285.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77787.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE56612.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015623603.1; XM_015768117.1.
DR RefSeq; XP_015623604.1; XM_015768118.1. [I6PL68-1]
DR RefSeq; XP_015623605.1; XM_015768119.1.
DR AlphaFoldDB; I6PL68; -.
DR SMR; I6PL68; -.
DR STRING; 4530.OS02T0232100-01; -.
DR PaxDb; I6PL68; -.
DR PRIDE; I6PL68; -.
DR EnsemblPlants; Os02t0232100-01; Os02t0232100-01; Os02g0232100. [I6PL68-1]
DR GeneID; 4328810; -.
DR Gramene; Os02t0232100-01; Os02t0232100-01; Os02g0232100. [I6PL68-1]
DR KEGG; osa:4328810; -.
DR eggNOG; ENOG502RMFV; Eukaryota.
DR HOGENOM; CLU_052740_0_0_1; -.
DR OMA; DEWINMV; -.
DR OrthoDB; 1383870at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; I6PL68; OS.
DR GO; GO:0005712; C:chiasma; IDA:UniProtKB.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IDA:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF14634; zf-RING_5; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Coiled coil; DNA recombination; Meiosis;
KW Metal-binding; Nucleus; Reference proteome; Transferase;
KW Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..303
FT /note="E3 ubiquitin-protein ligase CCNB1IP1 homolog"
FT /id="PRO_0000425731"
FT ZN_FING 3..42
FT /note="RING-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 201..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 119..184
FT /evidence="ECO:0000255"
FT VAR_SEQ 214..222
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:22792078"
FT /id="VSP_053850"
FT CONFLICT 303
FT /note="T -> TL (in Ref. 1; AFJ04809/AFJ04810)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 303 AA; 34409 MW; 71B3F78707E74C58 CRC64;
MKCNACWREL EGQAVSTTCG HLLCTEDAKK ILSNDAACPI CDQVLSKSHM RPVDTNPNDD
WTNMSMAGVS PQILMKSAYR SVMFYIGQKE LEMQYKMNRI VGQCRQKCEL MQAKFTEKLE
EVHTAYQKMA KKCQLMEQEV ENLSRDKQEL QEKFAEKSRQ KRKLDEMYDQ LRSEYESAKR
SAIQPANNYF PRAQPDLFSG VPNIMDSSDP LRQGLAGLPE TPGRRDEGWA PPPRQRRSTS
GPFELSAGSP AHNAAPPVDI RPRQPARPVF GTAMNNTSAA LRNMIISPVK RPQLSRNRPH
MFT
