HELI_HHV11
ID HELI_HHV11 Reviewed; 882 AA.
AC P10189; B9VQD2; Q09IC8;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 3.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=DNA replication helicase {ECO:0000255|HAMAP-Rule:MF_04030};
DE EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_04030};
GN Name=HELI {ECO:0000255|HAMAP-Rule:MF_04030}; OrderedLocusNames=UL5;
OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX NCBI_TaxID=10299;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA Perry L.J., Scott J.E., Taylor P.;
RT "The complete DNA sequence of the long unique region in the genome of
RT herpes simplex virus type 1.";
RL J. Gen. Virol. 69:1531-1574(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2826807; DOI=10.1128/jvi.62.2.444-453.1988;
RA McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P.,
RA Challberg M.D.;
RT "Structures of herpes simplex virus type 1 genes required for replication
RT of virus DNA.";
RL J. Virol. 62:444-453(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nonneuroinvasive mutant HF10;
RX PubMed=17218138; DOI=10.1016/j.micinf.2006.10.019;
RA Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.;
RT "Determination and analysis of the DNA sequence of highly attenuated herpes
RT simplex virus type 1 mutant HF10, a potential oncolytic virus.";
RL Microbes Infect. 9:142-149(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17 syn+;
RA Cunningham C., Davison A.J.;
RT "Herpes simplex virus type 1 bacterial artificial chromosome.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=KOS;
RX PubMed=2535726; DOI=10.1128/jvi.63.1.196-204.1989;
RA Olivo P.D., Nelson N.J., Challberg M.D.;
RT "Herpes simplex virus type 1 gene products required for DNA replication:
RT identification and overexpression.";
RL J. Virol. 63:196-204(1989).
CC -!- FUNCTION: Component of the helicase/primase complex. Unwinds the DNA at
CC the replication forks and generates single-stranded DNA for both
CC leading and lagging strand synthesis. The primase synthesizes short RNA
CC primers on the lagging strand that the polymerase elongates using
CC dNTPs. Possesses helicase-like motifs and therefore may act as the
CC helicase subunit of the complex. {ECO:0000255|HAMAP-Rule:MF_04030}.
CC -!- SUBUNIT: Associates with the primase and the primase-associated factor
CC to form the helicase-primase complex. {ECO:0000255|HAMAP-
CC Rule:MF_04030}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04030,
CC ECO:0000269|PubMed:2535726}.
CC -!- SIMILARITY: Belongs to the herpesviridae helicase family.
CC {ECO:0000255|HAMAP-Rule:MF_04030}.
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DR EMBL; X14112; CAA32341.1; -; Genomic_DNA.
DR EMBL; M19119; AAA45819.1; -; Genomic_DNA.
DR EMBL; DQ889502; ABI63467.1; -; Genomic_DNA.
DR EMBL; FJ593289; ACM62227.1; -; Genomic_DNA.
DR RefSeq; YP_009137079.1; NC_001806.2.
DR BioGRID; 971446; 2.
DR DIP; DIP-1094N; -.
DR IntAct; P10189; 1.
DR MINT; P10189; -.
DR ChEMBL; CHEMBL4630721; -.
DR PRIDE; P10189; -.
DR DNASU; 2703420; -.
DR GeneID; 2703420; -.
DR KEGG; vg:2703420; -.
DR Proteomes; UP000009294; Genome.
DR Proteomes; UP000180652; Genome.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IDA:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_04030; HSV_HELI; 1.
DR InterPro; IPR003840; DNA_helicase.
DR InterPro; IPR034711; HSV_HELI.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF02689; Herpes_Helicase; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; Helicase; Host nucleus; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..882
FT /note="DNA replication helicase"
FT /id="PRO_0000115845"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 97..104
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04030"
FT VARIANT 14
FT /note="K -> R (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 67
FT /note="H -> R (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 205
FT /note="L -> S (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 662
FT /note="V -> I (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 690
FT /note="V -> G (in strain: Nonneuroinvasive mutant HF10)"
FT CONFLICT 860
FT /note="E -> D (in Ref. 2; AAA45819)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 882 AA; 98715 MW; 38FF9C5DD831BFDB CRC64;
MAAAGGERQL DGQKPGPPHL QQPGDRPAVP GRAEAFLNFT SMHGVQPILK RIRELSQQQL
DGAQVPHLQW FRDVAALESP AGLPLREFPF AVYLITGNAG SGKSTCVQTI NEVLDCVVTG
ATRIAAQNMY AKLSGAFLSR PINTIFHEFG FRGNHVQAQL GQYPYTLTSN PASLEDLQRR
DLTYYWEVIL DLTKRALAAS GGEELRNEFR ALAALERTLG LAEGALTRLA PATHGALPAF
TRSNVIVIDE AGLLGRHLLT AVVYCWWMIN ALYHTPQYAA RLRPVLVCVG SPTQTASLES
TFEHQKLRCS VRQSENVLTY LICNRTLREY ARLSYSWAIF INNKRCVEHE FGNLMKVLEY
GLPITEEHMQ FVDRFVVPEN YITNPANLPG WTRLFSSHKE VSAYMAKLHA YLKVTREGEF
VVFTLPVLTF VSVKEFDEYR RLTHQPGLTI EKWLTANASR ITNYSQSQDQ DAGHMRCEVH
SKQQLVVARN DVTYVLNSQI AVTARLRKLV FGFSGTFRAF EAVLRDDSFV KTQGETSVEF
AYRFLSRLIF SGLISFYNFL QRPGLDATQR TLAYARMGEL TAEILSLRPK SSGVPTQASV
MADAGAPGER AFDFKQLGPR DGGPDDFPDD DLDVIFAGLD EQQLDVFYCH YTPGEPETTA
AVHTQFALLK RAFLGRFRIL QELFGEAFEV APFSTYVDNV IFRGCEMLTG SPRGGLMSVA
LQTDNYTLMG YTYARVFAFA DELRRRHATA NVAELLEEAP LPYVVLRDQH GFMSVVNTNI
SEFVESIDST ELAMAINADY GISSKLAMTI TRSQGLSLDK VAICFTPGNL RLNSAYVAMS
RTTSSEFLRM NLNPLRERHE RDDVISEHIL SALRDPNVVI VY
