ID   HELI_NPVAC              Reviewed;        1221 AA.
AC   P24307;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA replication helicase;
DE            EC=3.6.4.12;
DE   AltName: Full=P143;
GN   Name=HELI; ORFNames=ORF95;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=HR3;
RX   PubMed=1994581; DOI=10.1016/0042-6822(91)90500-b;
RA   Lu A., Carstens E.B.;
RT   "Nucleotide sequence of a gene essential for viral DNA replication in the
RT   baculovirus Autographa californica nuclear polyhedrosis virus.";
RL   Virology 181:336-347(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [3]
RP   MUTAGENESIS OF VAL-934, AND FUNCTION.
RX   PubMed=9887325; DOI=10.1006/viro.1998.9485;
RA   Liu G., Carstens E.B.;
RT   "Site-directed mutagenesis of the AcMNPV p143 gene: effects on baculovirus
RT   DNA replication.";
RL   Virology 253:125-136(1999).
RN   [4]
RP   INTERACTION WITH LEF-3 AND IE1.
RX   PubMed=15518813; DOI=10.1016/j.virol.2004.08.035;
RA   Ito E., Sahri D., Knippers R., Carstens E.B.;
RT   "Baculovirus proteins IE-1, LEF-3, and P143 interact with DNA in vivo: a
RT   formaldehyde cross-linking study.";
RL   Virology 329:337-347(2004).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16103143; DOI=10.1128/jvi.79.17.10915-10922.2005;
RA   Chen Z., Carstens E.B.;
RT   "Identification of domains in Autographa californica multiple
RT   nucleopolyhedrovirus late expression factor 3 required for nuclear
RT   transport of P143.";
RL   J. Virol. 79:10915-10922(2005).
CC   -!- FUNCTION: Essential for initiation of viral DNA replication, it may
CC       contribute to other functions such as controlling the switch to the
CC       late phase and leading to the inhibition of host protein synthesis.
CC       Required for late and very late gene expression.
CC       {ECO:0000269|PubMed:16103143, ECO:0000269|PubMed:1994581,
CC       ECO:0000269|PubMed:9887325}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with IE1 and LEF-3. {ECO:0000269|PubMed:15518813}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:16103143}.
CC       Note=LEF-3 is responsible for transporting the helicase to the host
CC       nucleus. {ECO:0000269|PubMed:16103143}.
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DR   EMBL; M57687; AAA67907.1; -; Genomic_DNA.
DR   EMBL; L22858; AAA66725.1; -; Genomic_DNA.
DR   PIR; A38499; HJNVAV.
DR   RefSeq; NP_054125.1; NC_001623.1.
DR   PRIDE; P24307; -.
DR   GeneID; 1403928; -.
DR   KEGG; vg:1403928; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0039715; C:nuclear viral factory; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IDA:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR006824; DNA_helicase_Baculovir.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04735; Baculo_helicase; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; DNA-binding; Early protein; Helicase;
KW   Host nucleus; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1221
FT                   /note="DNA replication helicase"
FT                   /id="PRO_0000132853"
FT   DNA_BIND        967..981
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MOTIF           692..701
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   BINDING         917..924
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         934
FT                   /note="V->M: Defective in the synthesis of viral DNA, late
FT                   protein synthesis and the shutoff of host protein synthesis
FT                   at the nonpermissive temperature (mutant ts8)."
FT                   /evidence="ECO:0000269|PubMed:9887325"
FT   CONFLICT        126
FT                   /note="F -> S (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1149
FT                   /note="F -> L (in Ref. 1; AAA67907)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1221 AA;  143214 MW;  090E199855882D1B CRC64;
     MIDNILQFFL KNVPQDKTYE INNLQDANHL IIRNTRTGTR RLFEYVNNFQ QFLNTIRNNF
     NGPCAKHDMG ASCEDTEEPA EKHAAQTLDG HDWVLESNDF CIFVKPFILK KHYDIIQKYI
     NFEDFFKSTD PGYINKCVQA GDYYYWPNWP KKQAFSFNGW QLFLNIKFGI VIEPTIPIIH
     NKKLGPVDLF VFDPKCFLNV ELSLRTNHDP PQTLFVNGKT KFDDSHEDLF ILKMADGTVA
     TCKVNGELVN SDKNFFNYIR DDINLEECIT VPKYKHIVNV NLKSLRVFEN NNFDKNDVDL
     SDTRSRKPRI VPIISASSEN ADYIQTQINL GLIAIHENMV KVLATHERAN DPNLLQQYFE
     KSKFKNFDFL IYVLWKILTK NENFSYRETD IKLFLELLCE SLFACDKEAL NEALKRCEPY
     KKQEKIVFNR ACNHWFDFDD TKLCVSLGYY FGIHYMIYLT QSAKNEILDH DELWAYTYEN
     VMALNLPPDI VCKGFFRKLE NVVTGVNLVF NGKHYQIVKK EDDLFKLTKS NCYKLSNIKF
     NNWKYLYLTT HGVYNVFTNS FHSSCPFLLG TTLPQTFKKP TDEKYLPEDA FNYMLSTSAD
     ELSIYRTYHI AKMCRDVKML KTNTAIVNYM GNCNTCQADM RVALNNLFRD LWNLDDENLI
     TLALYVNKNR VSDMLHNLKC KPCRSTVSGS RPKCKCYKKI KINRKALKVC LMADMFGNDA
     ELSELIWMLI FTNKTYVSTT LIRTNSEFVN QHGEFFSKEH NKIIQYLYRT IHKIEYVDML
     MDKFNDKRLF LTELRDDVAR EPDVQFEESD NISKFYTHHA DALMILKKYN VWWDKIILAR
     STDDLPTWLT RFYMRIIMSK VDLKEYSYNY LKKIVEGYLY FKRFTNFNHA NAIMLMHFAA
     SLAIPVDYGK KAIYMPGEPG SGKSSFFELL DYLVLMHKFD DDNHSGESNK ETSDKEVSKL
     NSQLYTINEL KQCSESYFKK HADSSKSDSK SRKYQGLLKY EANYKMLIVN NKPLYVDDYD
     DGVQDRFLIV YTNHKFVDSV KFAGSVYEHI KSKQFPIESM YYESLVTPVR LFLSHVLMYR
     RDPKTGFVVY KTLLSNDPMH KHNLMCLSTN NSPLYALIYI LNIKTVRSAT ITIGEDKMEE
     MIGIAVQHFK NFLHPSFVQY NYKKNINASS SKSFVFNEQV LLQQIKNKFK NNYNKTTNVF
     YNMTMALNRN DLNTSVPNFV C