ID   HELS_HALS3              Reviewed;         783 AA.
AC   B0R7Q2;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=OE_4325F;
OS   Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=478009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29341 / DSM 671 / R1;
RX   PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; AM774415; CAP14771.1; -; Genomic_DNA.
DR   RefSeq; WP_010903767.1; NC_010364.1.
DR   AlphaFoldDB; B0R7Q2; -.
DR   SMR; B0R7Q2; -.
DR   EnsemblBacteria; CAP14771; CAP14771; OE_4325F.
DR   GeneID; 5953740; -.
DR   KEGG; hsl:OE_4325F; -.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   PhylomeDB; B0R7Q2; -.
DR   Proteomes; UP000001321; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..783
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_1000124580"
FT   DOMAIN          34..209
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          242..443
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   REGION          744..783
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           154..157
FT                   /note="DEAH box"
FT   BINDING         29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         47..54
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   783 AA;  83580 MW;  AD9F1D7F5160C649 CRC64;
     MRVADVPGLP GGVADHFEGE GVEELYPPQA EAVERGVTEG ANLVASVPTA SGKTLIAQLA
     MLSAIAEGGD SPTFSGDGTA LYIVPLRALA GEKAQEFEAF ERFGLSVGVS TGNYERDGAR
     LADNDIVVAT SEKVDSLVRN GAGWIDDLSC VVADEVHLVD DDHRGPTLEV TLAKLRQQVA
     DLQVVALSAT VGNAGELAAW LDAELVDSDW RPIELRTGVH YGQSLHYDDG TQAELSVGSG
     SQTAAVVADT LADDGSTLVF VNSRRNAEAS ARRLADVTGN ALSSAERERL ADIAAEIRGV
     SDTETSDELA DAVASGAAFH HAGLAREHRE LVEEAFRDRL VKAVSATPTL AAGVNTPARR
     VVVRDWQRYD GTAGGMQPLD VLEVHQMFGR AGRPGLDPYG EAVLLANSHD ELEELFDRYV
     YADPEPVRSK LAAEPALRTH VLAAIATGFT TTEDGLHEFL GGTLYATQTD DTGRLRSVTG
     DVLRYLDRNG FVERDGAALR ATATGQLVSR LYVDPMSAAT IIDGLRDAAR DATETDDEGA
     FRPASELGDD AALPADASVE PTPLGLYHLV SRTPDMYELY LRSGDREQYT EVAYEHEDEL
     LGATPREEQA EFEDWLSALK TARLMADWAS ELDEERIAER YDVGPGDIRG KVETAEWLLN
     AAERLAGELD VECGPAVREA RKRVQYGVRE ELLGLAGVRN VGRKRARRLY NAGVESRADL
     RNADKGVVLG AVRGRAATAE RILETVGHPD PGMDGVAADT DAAPESGGEA GGDEGQASLG
     DFS