HELS_METAC
ID HELS_METAC Reviewed; 730 AA.
AC Q8TL39;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=MA_3203;
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR EMBL; AE010299; AAM06574.1; -; Genomic_DNA.
DR RefSeq; WP_011023138.1; NC_003552.1.
DR AlphaFoldDB; Q8TL39; -.
DR SMR; Q8TL39; -.
DR STRING; 188937.MA_3203; -.
DR EnsemblBacteria; AAM06574; AAM06574; MA_3203.
DR GeneID; 1475096; -.
DR KEGG; mac:MA_3203; -.
DR HOGENOM; CLU_006553_3_0_2; -.
DR InParanoid; Q8TL39; -.
DR OMA; MFLNANI; -.
DR OrthoDB; 9752at2157; -.
DR PhylomeDB; Q8TL39; -.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..730
FT /note="ATP-dependent DNA helicase Hel308"
FT /id="PRO_0000102106"
FT DOMAIN 33..199
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT DOMAIN 232..433
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT REGION 707..730
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 144..147
FT /note="DEAH box"
FT BINDING 28
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ SEQUENCE 730 AA; 80958 MW; CB557279E92BD889 CRC64;
MKIESLDLPD EVKRFYENSG IPELYPPQAE AVEKGLLEGK NLLAAIPTAS GKTLLAELAM
LKSVLAGGKA LYIVPLRALA SEKFRRFQDF SELGIRVGIS TGDYDRRDEG LGINDIIVAT
SEKTDSLLRN ETAWMQEISV VVVDEVHLID SADRGPTLEV TLAKLRKMNP FCQILALSAT
VGNADELAAW LDAELVLSEW RPTDLMEGVF FDGTFFCKDK EKLIEQPTKD EAINLVLDTL
REGGQCLVFE SSRKNCMGFA KKATSAVKKT LSAEDKEKLA GIADEILENS ETDTASVLAS
CVRAGTAFHH AGLTSPLREL VETGFREGYV KLISSTPTLA AGLNLPARRV IIRSYRRYSS
DSGMQPIPVL EYKQMAGRAG RPRLDPYGEA VLLAKSYEEL LFLFEKYIEA GAEDIWSKLG
TENALRTHVL STISNGFART KEELMDFLEA TFFAYQYSNF GLSVVVDECL NFLRQEGMLE
QDSDALISTM FGKLVSRLYI DPLSAALIAK GLREAGTLTE LTLLHLVCST PDMRLMYMRS
QDYQDINDFV MAHAEEFSKV PSPFNIVEYE WFLSEVKTSL LLMDWIHEKP ENEICLKFGT
GEGDIHTTAD IAEWIMHVAT QLARLLDLKG AKEAAELEKR IHYGAGPELM DLLDIRGIGR
VRARKLYGAG FKSTADLAGA TPEKVAALVG PKIAERIFRQ IGRREAVSEI SDSERLEKSS
QDGQSTISDF