HELS_METAR
ID HELS_METAR Reviewed; 740 AA.
AC Q0W6L1;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN OrderedLocusNames=UNCMA_22030; ORFNames=RCIX575;
OS Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanocellales; Methanocellaceae; Methanocella.
OX NCBI_TaxID=351160;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX PubMed=16857943; DOI=10.1126/science.1127062;
RA Erkel C., Kube M., Reinhardt R., Liesack W.;
RT "Genome of rice cluster I archaea -- the key methane producers in the rice
RT rhizosphere.";
RL Science 313:370-372(2006).
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR EMBL; AM114193; CAJ35982.1; -; Genomic_DNA.
DR RefSeq; WP_012036523.1; NC_009464.1.
DR AlphaFoldDB; Q0W6L1; -.
DR SMR; Q0W6L1; -.
DR STRING; 351160.RCIX575; -.
DR EnsemblBacteria; CAJ35982; CAJ35982; RCIX575.
DR GeneID; 5144228; -.
DR KEGG; rci:RCIX575; -.
DR PATRIC; fig|351160.9.peg.2253; -.
DR eggNOG; arCOG00553; Archaea.
DR OMA; MFLNANI; -.
DR OrthoDB; 9752at2157; -.
DR Proteomes; UP000000663; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..740
FT /note="ATP-dependent DNA helicase Hel308"
FT /id="PRO_1000124587"
FT DOMAIN 33..204
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT DOMAIN 236..436
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT REGION 716..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 149..152
FT /note="DEAH box"
FT COMPBIAS 723..740
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 28
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ SEQUENCE 740 AA; 81052 MW; 4073BF8A93A1991E CRC64;
MKIAELNLPQ ALKDFYTGSG IPELYPPQAE AIRQGLLDGK NLLAAIPTAS GKTLLAEMAM
LKSIAEGGKA IYIVPLKALA SEKYDRFLEF SKLPIKPDGV KVGIATGDFD SRDEYLGEKD
IIVATSEKTD SLLRNGASWL SGLSVVVADE VHLIDSPNRG PTLEVTLAKL RKINVNLQIL
ALSATIGNAK ALAKWMDAAL VQSEWRPTTL KEGVFYGRAI TFKKEKRTVN NAGPDEVNSL
VADTLEEGGQ CLVFANTRKS SESIAQKVAR SLSKKLQPAE KEQLAKLKQD VLRHAETDTC
EKLAECVGNG VAFHHAGLKG EHRRIVEDGF RQNILKVIAC TPTLAAGLNL PARRVIIRDY
KRFDVNYGSV PIPVLEYKQM AGRAGRPRLD PYGEAVLIAK NYDEFGELME NYINADPEHI
TSKLGTEPAM RAHALSAVAT DFCRSRQDLK AFMDTTFFAY QRGDLSHVID NVLNFLLEEN
MIIESKGGSL KATDLGSLVS KLYIDPLSAA LIAEGLEKAK KRPDVAEFGL LHLICSTPDV
KSLYLRRGDY SWIIRYADEH ASDFLSDVPD SYGDDVEFEQ FLAGVKTAAL AEMWINEKSE
EAITTFFNIG PGDIRNLMET CTWLMHGTAE ISALLGAPAT RTARELAIRI ENGASRELLD
LITLKGVGRV RARKLYDAGY TSRDKLKAAE IPAIAAIPGI GDKLAVSIMS QLGRKVDHTP
PETEEQPQVS GQSTLFSFDG