ID   HELS_METB6              Reviewed;         723 AA.
AC   A7IB61;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=Mboo_2458;
OS   Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanoregulaceae; Methanoregula.
OX   NCBI_TaxID=456442;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21154 / JCM 14090 / 6A8;
RX   PubMed=25998264; DOI=10.1099/mic.0.000117;
RA   Braeuer S., Cadillo-Quiroz H., Kyrpides N., Woyke T., Goodwin L.,
RA   Detter C., Podell S., Yavitt J.B., Zinder S.H.;
RT   "Genome of Methanoregula boonei 6A8 reveals adaptations to oligotrophic
RT   peatland environments.";
RL   Microbiology 161:1572-1581(2015).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; CP000780; ABS56972.1; -; Genomic_DNA.
DR   RefSeq; WP_012108036.1; NC_009712.1.
DR   AlphaFoldDB; A7IB61; -.
DR   SMR; A7IB61; -.
DR   STRING; 456442.Mboo_2458; -.
DR   EnsemblBacteria; ABS56972; ABS56972; Mboo_2458.
DR   GeneID; 5410542; -.
DR   KEGG; mbn:Mboo_2458; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   OrthoDB; 9752at2157; -.
DR   Proteomes; UP000002408; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..723
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_1000124581"
FT   DOMAIN          33..196
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          230..416
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   REGION          693..723
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           141..144
FT                   /note="DEAH box"
FT   COMPBIAS        707..723
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   723 AA;  79915 MW;  B957AE438A5DEE19 CRC64;
     MQIQDLAIPE PLRQQYLGLG IRELYPPQAA CVERGLLDGK NLLVAIPTAS GKTLIAEMAM
     HRHIANGGKC LYIVPLKALA SEKYEEFGNK GVKVGLSTGD LDRRDDALGK NDIIVATSEK
     VDSLLRNGAR WIPDITLVVI DEIHLIDSPD RGPTLEMVIA KMRSKNPGMQ LIGLSATIGN
     PKVLAGWLDA ELVTSSWRPV DLRQGVFYDN RIQFAERMRP VKQVSKNYDD LNLCLDTIAE
     GGQCLVFVSS RRNAEAFAKR AAGAIKSEDA ALAACAERLL EGTPTEMVKT LAACVAKGAA
     FHHAGLSRKE RSIVEEAFRK NLLKCISSTP TLAAGLNLPA RRVIIRDYLR FSAGEGMQPI
     PVSEYRQMAG RAGRPRLDPY GEAVLIAKEA EQVPELFEVY IEAEAEDVHS RIAEPTALYT
     HVLSLVASGF AGTRGELTEF MNRSFYVHEH KQGRLIHRAI DEALQFLITA EMVVEVGEHI
     GATELGTLVS RMYIDPRSAF AIVTTLREQE KYADLGLIQL ICTTPDMPTL YAKNADLPAL
     SRMLEVRGAD IWLPPPLDDD AAETYYRAVK TAMLLSDWTD ELSEEKICER YGVGPGDVFG
     MVENINWLLH ATSQLARMFV PKFYGQIADC EICMKNGIRR ELLPLVRLRG IGRVRARRLF
     NNGITSPEEL SRHKKEDLVK ILGSGIAEQV LEQLHPSKDT GKKEPPSGDK NTNPGQSTLF
     HFG