ID   HELS_METBF              Reviewed;         729 AA.
AC   Q465R3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   OrderedLocusNames=Mbar_A3508;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; CP000099; AAZ72379.1; -; Genomic_DNA.
DR   RefSeq; WP_011308418.1; NC_007355.1.
DR   AlphaFoldDB; Q465R3; -.
DR   SMR; Q465R3; -.
DR   STRING; 269797.Mbar_A3508; -.
DR   EnsemblBacteria; AAZ72379; AAZ72379; Mbar_A3508.
DR   GeneID; 3624906; -.
DR   KEGG; mba:Mbar_A3508; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   OrthoDB; 9752at2157; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..729
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_1000124582"
FT   DOMAIN          33..199
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          232..426
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   REGION          706..729
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           144..147
FT                   /note="DEAH box"
FT   BINDING         28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   729 AA;  80841 MW;  4EA68564B5F2F6D2 CRC64;
     MKIESLDLPD EVKQFYLNSG IMELYPPQAE AVEKGLLEGR NLLAAIPTAS GKTLLAELAM
     LKSILAGGKA LYIVPLRALA SEKFRRFREF SELGIRVGIS TGDYDLRDEG LGVNDIIVAT
     SEKTDSLLRN ETVWMQEISV VVADEVHLID SPDRGPTLEV TLAKLRKMNP SCQILALSAT
     VGNADELAVW LEAELVVSEW RPTELLEGVF FNGTFYCKDR EKTVEQSTKD EAVNLALDTL
     KKDGQCLVFE SSRKNCMAFA KKAASTVKKT LSAEDRNALA GIADEILENS ETDTSTNLAV
     CIRSGTAFHH AGLTTPLREL VEDGFRAGRI KLISSTPTLA AGLNLPARRV IIRNYRRYSS
     EDGMQPIPVL EYKQMAGRAG RPRLDPYGEA VLVAKSYKEF VFLFENYIEA NAEDIWSKLG
     TENALRTHVL STISNGFART YDELMDFLEA TFFAFQYSNF GLSTVVNECL NFLRQEGMLE
     KDDALIPTSF GKLVSRLYID PLSAARIAKG LKGAKSLSEL TLLHLVCSTP DMRLLYMRSH
     DYQDINDYVM AHASEFVKVP SPFDTTEYEW FLGEVKTSLL LLDWIHEKSE NEICLKFGTG
     EGDIHSIADI AEWIMHVTSQ LAGLLDLKGA REAAELEKRI HYGAAPELID LLNIRGIGRV
     RARKLYEAGF KSSAELAEVD PEKVAALLGP KIADRIFKQI RGRGTSSGII ASEPPEKSPY
     SGQKTISDY