HELS_METJA
ID HELS_METJA Reviewed; 1195 AA.
AC Q58524;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
DE Contains:
DE RecName: Full=Endonuclease PI-MjaHel;
DE EC=3.1.-.-;
DE AltName: Full=Mja Hel intein;
DE AltName: Full=Mja Pep3 intein;
GN Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=MJ1124;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR EMBL; L77117; AAB99126.1; -; Genomic_DNA.
DR PIR; C64440; C64440.
DR RefSeq; WP_010870635.1; NC_000909.1.
DR AlphaFoldDB; Q58524; -.
DR SMR; Q58524; -.
DR STRING; 243232.MJ_1124; -.
DR PRIDE; Q58524; -.
DR EnsemblBacteria; AAB99126; AAB99126; MJ_1124.
DR GeneID; 1452020; -.
DR KEGG; mja:MJ_1124; -.
DR eggNOG; arCOG00553; Archaea.
DR eggNOG; arCOG03157; Archaea.
DR HOGENOM; CLU_279743_0_0_2; -.
DR InParanoid; Q58524; -.
DR OMA; CLNANTE; -.
DR OrthoDB; 9752at2157; -.
DR PhylomeDB; Q58524; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.300; -; 3.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 3.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Autocatalytic cleavage; DNA damage; DNA repair; DNA-binding;
KW Endonuclease; Helicase; Hydrolase; Intron homing; Nuclease;
KW Nucleotide-binding; Protein splicing; Reference proteome.
FT CHAIN 1..337
FT /note="ATP-dependent DNA helicase Hel308, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013299"
FT CHAIN 338..838
FT /note="Endonuclease PI-MjaHel"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013300"
FT CHAIN 839..1195
FT /note="ATP-dependent DNA helicase Hel308, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013301"
FT DOMAIN 26..196
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT DOMAIN 451..584
FT /note="DOD-type homing endonuclease"
FT MOTIF 143..146
FT /note="DEAH box"
FT BINDING 20
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT BINDING 39..46
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ SEQUENCE 1195 AA; 138674 MW; 87C9CF1A87EFB52D CRC64;
MDKILEILKD FGIVELRPPQ KKALERGLLD KNKNFLISIP TASGKTLIGE MALINHLLDG
NKNPTNKKGI FIVPLKALAS EKYEEFKSKY ERYGLRIALS IGDYDEDEDL SKYHLIITTA
EKLDSLWRHK IDWINDVSVV VVDEIHLIND ETRGGTLEIL LTKLKEFNVQ IIGLSATIGN
PDELAEWLNA ELIVDDWRPV ELKKGIYKNE AIEFINGEIR EIKAVDNNDI YNLVVDCVKE
GGCCLVFCNT KRNAVNEAKK LNLKKFLTEE EKIRLKEIAE EILSILEPPT EMCKTLAECI
LNGSAFHHAG LTYQHRKIVE DAFRKRLIKV ICCTPTLCLN ANTEILQESG FRKITELNKD
EKVFALCGKE IKPVDGWKVH KTPQHEYNIV VKTVNGLEIT TTPNHIFLVK ENGSLKEKEA
KDLKVGDYVA TVDRIRVKEK DIDLSNGDLY FIGYFIGDGY TGVIEKNTLK ATPDLAFNPK
YPPNFDDSEL HKKYFLKCRI SKGVAHYIYS KKLRKIFNKL NMLTKDNKNI DAFCNLPLDK
LAYLIAGLFD SDGYIYLNRK NIEFYSISEK LVEQLQFVLL RFGIHSSIRK KKTKTMVSPT
NGKEYKCKDI YVLTIRDFMS IKRFYENIPL RHEEKRRKLE EIIKNKEIGQ IPSEFVALRF
TPIAKIWCDC GFSVDLTMFK PRTKRQRELN KKRVKLLFEL LDGKKLITNY KEYYSKRKNP
YFDFIVREKI NGNNYYSLNE KGRVLMSLLN KHIKDKENLE EMYNFLVNLE KCPICGKPIH
KEMRYSWKKE CYDGDIYWDR IKEIKKIKVN DKYAYDIELP DDGSNSHYIV ANGFIVHNSA
GLNLPCRRAI VKDLTRFTNK GMRYIPIMEI QQCIGRAGRP GLDPYGEGII VAKNDRDYLR
AYQALTQKPE PIYSKLSNQA VLRTQLLGLI ATGEIRDEYD LEWFIRNTFY AHQYGNLREV
AKNINEVIRF LEENEFIIDF MPTELGKRVS ELYIDPLSAK FIIDGLEEME NEEEIYYLYL
ISKTLEMMPN LRVYNSEELN LIDEMDSLGI KSFEIEDLEA FKTAKMLYDW INEVPEDEIL
KRYKIEPGIL RYKVENAVWI MHALKEIAKL IGKSSDIPEK LEIRLEYGAK EDIIELLSIK
YIGRVRARKL YNAGIRSIED IINNPSKVAS IIGEKIAKKI LDELGVKFGQ QKLSF
