ID   HELS_METMA              Reviewed;         730 AA.
AC   Q8PZR7;
DT   25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=MM_0425;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE008384; AAM30121.1; -; Genomic_DNA.
DR   RefSeq; WP_011032378.1; NC_003901.1.
DR   AlphaFoldDB; Q8PZR7; -.
DR   SMR; Q8PZR7; -.
DR   STRING; 192952.MM_0425; -.
DR   EnsemblBacteria; AAM30121; AAM30121; MM_0425.
DR   GeneID; 24877056; -.
DR   KEGG; mma:MM_0425; -.
DR   PATRIC; fig|192952.21.peg.513; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..730
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_0000102107"
FT   DOMAIN          33..199
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          232..433
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   MOTIF           144..147
FT                   /note="DEAH box"
FT   BINDING         28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   730 AA;  81074 MW;  58E7AFB5752BD48F CRC64;
     MKIESLDLPD EIKRFYENSG ILELYPPQAE AVEKGLLEGK NLLAAIPTAS GKTLLAELAM
     LKSVLNGGKA LYIVPLRALA SEKFRRFQEF SVLGMRVGIS TGDYDRRDEG LGINDIIVAT
     SEKTDSLLRN ETAWMQEISV VVADEVHLID SPDRGPTLEI TLSKLRRMNP SCQVLALSAT
     VGNADELAAW LDAELVLSEW RPTDLMEGVF YNGIFYCKDK EKPVGQPTKD EAVNLVLDTI
     KEGGQCLVFE SSRKNCMGFA KKAVSAVKKT LSNEDRETLA GIADEIIENS ETDVSSVLAT
     CVRSGTAFHH AGLTTPLREL VENGFREGRI KIISSTPTLA AGLNLPARRV IIRSYRRYSS
     DSGMQPIPVL EYKQMAGRAG RPRLDPYGEA VLLAKSYEEF VFLFEKYIEA GAEDIWSKLG
     TENALRTHIL STISNGFART REELMDFLEA TFFAFQYSNF GLSAVVDECL DFLRREGMLE
     KDPDALVSTV FGKLVSRLYI DPLSAALIAK GLREAGTLTE LTLLHLICST PDMRLMYMRS
     QDYQEVNDYV MAHAGEFSKV PNPFNIAEYE WFLGEVKTSL LLMDWIHEKP ENEICLKFGI
     GEGDIHATAD IAEWIMHVTA QLAGLLDLKG AKEASELEKR IRYGAAPELM DLLDIRSVGR
     VRARKLYEAG FKSTAELAAA SPEHIAVLVG PKITERIFKQ IGRREAVSEF SDIEPLEKGS
     SDGQRTISDY