ID   HELS_NATPD              Reviewed;         747 AA.
AC   Q3IU46;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=NP_0492A;
OS   Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM
OS   8858 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Natronomonas.
OX   NCBI_TaxID=348780;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB
RC   2260 / Gabara;
RX   PubMed=16169924; DOI=10.1101/gr.3952905;
RA   Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J.,
RA   Oesterhelt D.;
RT   "Living with two extremes: conclusions from the genome sequence of
RT   Natronomonas pharaonis.";
RL   Genome Res. 15:1336-1343(2005).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; CR936257; CAI48337.1; -; Genomic_DNA.
DR   RefSeq; WP_011321973.1; NC_007426.1.
DR   AlphaFoldDB; Q3IU46; -.
DR   SMR; Q3IU46; -.
DR   STRING; 348780.NP_0492A; -.
DR   PRIDE; Q3IU46; -.
DR   EnsemblBacteria; CAI48337; CAI48337; NP_0492A.
DR   GeneID; 3702913; -.
DR   KEGG; nph:NP_0492A; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   OrthoDB; 9752at2157; -.
DR   Proteomes; UP000002698; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..747
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_1000080862"
FT   DOMAIN          34..200
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          234..434
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   REGION          711..747
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           145..148
FT                   /note="DEAH box"
FT   BINDING         29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         47..54
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   747 AA;  81108 MW;  AE955549EC57148E CRC64;
     MDVSEVSGVP PWFADHLREE GIESLYPPQA AAVDAGVADG ESLVASVPTA SGKTLVAQLA
     MLSAIDRGGT ALYIVPLRAL ASEKREEFAA FEEYGLSVGV TTGSYEDTGE WLADKDIIVA
     TSEKVDSLVR NGAPWIDDLD CVVADEVHLV DDEHRGPTLE VTLAKLRRVN PNLQVVALSA
     TVGNAGEMAE WLDAELVDSS WRPIELRKGV HYGQALHFGD GTQQELPVQR TEKPTEAVVR
     ETLDEGGSTL VFVNSRRNAE GAAKRLAKTT VDGLDADERA ALSSLAEEIR AVSDTETSDD
     LADCVEQGAA FHHAGCSSEH RSLVEDAFRD RLIKTICATP TLAAGVNTPA RRVVVRDWRR
     YSGDAGGMQP LSVLEVHQMM GRAGRPGRDP YGEAVLLADS HDELDELLDR YVWADPEPVE
     SKLAREPSMR THLLATVASG FADSRSALLS FLDRTLYATQ YRHGDGEDNL ERVVDTTLSY
     LETNGFIDRD GDAIEATDLG HTVSRLYLDP MSAAEIIDGL ADAASPTAMG LYQLVARTPD
     MYELYLRSGD REEYTMLAYE REAELLGELP SEFEEGRFED WLSALKTARM LEDWASELDE
     DDIAERYGVG PGDIRGKVDT AEWLLGAAES LASEQDLADV SAIREARKRV EHGVGEELID
     LAGVRGVGRK RARRLYDAGI ETRADLRNAD KSVVLAALRG REQTAENVLE AAGHQQPEMD
     GVTPDADVKE SAAAAGTDDG QANLGDF