ID   HELS_PYRAB              Reviewed;         715 AA.
AC   Q9V0A9; G8ZI46;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   OrderedLocusNames=PYRAB08810; ORFNames=PAB0592;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
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DR   EMBL; AJ248285; CAB49795.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE70287.1; -; Genomic_DNA.
DR   PIR; B75135; B75135.
DR   RefSeq; WP_010868004.1; NC_000868.1.
DR   AlphaFoldDB; Q9V0A9; -.
DR   SMR; Q9V0A9; -.
DR   STRING; 272844.PAB0592; -.
DR   EnsemblBacteria; CAB49795; CAB49795; PAB0592.
DR   GeneID; 1496231; -.
DR   KEGG; pab:PAB0592; -.
DR   PATRIC; fig|272844.11.peg.931; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   OrthoDB; 9752at2157; -.
DR   PhylomeDB; Q9V0A9; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00278; HhH1; 2.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..715
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_0000102109"
FT   DOMAIN          33..197
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          226..422
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   MOTIF           1..29
FT                   /note="Q motif"
FT   MOTIF           145..148
FT                   /note="DEAH box"
FT   BINDING         28
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         46..53
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   715 AA;  82006 MW;  611401E623690EF4 CRC64;
     MKVGELNVSE KIKEILRERG IEELYPPQAE ALTSGVLEGE NLLVAIPTAS GKTLIAEIAI
     ANKLLEEGGK AVYIVPLKAL AEEKFREFKD WERLGLKVAM ATGDYDSKDE WLGKYDIIIA
     TAEKFDSLLR HGSSWIRDVK MLVIDEIHLI GSRDRGATLE FIITQMLGRA QIIGLSATIG
     NPEELAEWLN AKLIRSDWRP VKLRKGVFYQ GFVFWEDGGS ERYNSWEELV YDAVKKGKGA
     LVFVNMRRKA EKTALELAKK VKNYLDRKEL RELRELAESL EENPTNEKLA KALLSGVAFH
     HAGLGRDERV LVEDNFRKGL IKVVVATPTL SAGINTPAFR VIIRDTWRYS EFGMERIPVL
     EVQQMMGRAG RPRYDEVGEA IIVSTTEEPS LVIDHYIKGK PEKLFSQLSN ESILRSQILA
     LIATFGYSEF KEIYDFLERT FYAHQGKDPY MLEEKIRRII YFLLENEFIE VTLEDEIKPL
     PLGVRTTKLY IDPMTAKIFK DTLPRIEKNP NPLGIFHMIS LAPDLTPLSY SKRETSMLED
     EYYSLMDRLY FELDYENERK FFRAFKTALV LNAWINEVPE GEIVERFNVE PGDIYRIVET
     AEWLIYSLGE IAKVLEASQE VVDYVNTLRL RVKHGIREEL IPLMELPMVG RKRARALYNA
     GFKDLESIRN AKPSELLRIE GIGAKIVEGI FKYLGKEVKI TERPRKGTLD YFLNP