ID   HELS_SULIR              Reviewed;         715 AA.
AC   F0NDL2;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_00442};
GN   Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=SiRe_0250;
OS   Sulfolobus islandicus (strain REY15A).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=930945;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=REY15A;
RX   PubMed=21278296; DOI=10.1128/jb.01487-10;
RA   Guo L., Brugger K., Liu C., Shah S.A., Zheng H., Zhu Y., Wang S.,
RA   Lillestol R.K., Chen L., Frank J., Prangishvili D., Paulin L., She Q.,
RA   Huang L., Garrett R.A.;
RT   "Genome analyses of icelandic strains of Sulfolobus islandicus, model
RT   organisms for genetic and virus-host interaction studies.";
RL   J. Bacteriol. 193:1672-1680(2011).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=REY15A / E233S;
RX   PubMed=22062475; DOI=10.1016/j.dnarep.2011.10.009;
RA   Hong Y., Chu M., Li Y., Ni J., Sheng D., Hou G., She Q., Shen Y.;
RT   "Dissection of the functional domains of an archaeal Holliday junction
RT   helicase.";
RL   DNA Repair 11:102-111(2012).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=REY15A;
RX   PubMed=23356319; DOI=10.1042/bst20120285;
RA   Zhang C., Tian B., Li S., Ao X., Dalgaard K., Gokce S., Liang Y., She Q.;
RT   "Genetic manipulation in Sulfolobus islandicus and functional analysis of
RT   DNA repair genes.";
RL   Biochem. Soc. Trans. 41:405-410(2013).
CC   -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC       involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC       Rule:MF_00442}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   -!- DISRUPTION PHENOTYPE: Essential, it cannot be disrupted.
CC       {ECO:0000269|PubMed:22062475, ECO:0000269|PubMed:23356319}.
CC   -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00442}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP002425; ADX84345.1; -; Genomic_DNA.
DR   RefSeq; WP_014511885.1; NC_017276.1.
DR   AlphaFoldDB; F0NDL2; -.
DR   SMR; F0NDL2; -.
DR   STRING; 930945.SiRe_0250; -.
DR   EnsemblBacteria; ADX84345; ADX84345; SiRe_0250.
DR   GeneID; 12417144; -.
DR   KEGG; sir:SiRe_0250; -.
DR   eggNOG; arCOG00553; Archaea.
DR   HOGENOM; CLU_006553_3_0_2; -.
DR   OMA; MFLNANI; -.
DR   Proteomes; UP000002664; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00442; Helicase_Hel308; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR022965; Helicase_Hel308.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..715
FT                   /note="ATP-dependent DNA helicase Hel308"
FT                   /id="PRO_0000429030"
FT   DOMAIN          40..203
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   DOMAIN          236..442
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   MOTIF           152..155
FT                   /note="DEAH box"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         35
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT   BINDING         53..60
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ   SEQUENCE   715 AA;  81379 MW;  0D34C846613A6A46 CRC64;
     MSLELEWMSI DDLKLPSNVI DIIKNRGIKK LNPPQTEAVK KGLLDGNRLL LTSPTGSGKT
     LIAEMGIISF LLKNGGKAIY VTPLRALTNE KYLTFKDWES IGFKVAMTSG DYDTDDAWLK
     NYDIIITTYE KLDSLWRHRP DWLNEANYFV LDELHYLNDP ERGPVVESVT IRAKRRNLLA
     LSATISNYKQ IAKWLGAEPV ATNWRPVPLM EGVMYPERKK KEYTILFRDN TTRKVHGDDA
     IIAYTLDSLS KNGQVLVFRN SRKMAESTAL KIANYMNFVS LDEKAISEIL NQLDDIEEGG
     SDEKELLKSL ISKGVAYHHA GLSKALRDII EESFRKRKIK VIVATPTLAA GVNLPARTVI
     IGDIYRFNRK IVGYYDEIPV MEYKQMSGRA GRPGFDQIGE SIIVVRDKED VDRVFKKYIL
     SDVEPIESKL GSERAFYTFL LGILSAEGSL SEKQLEGFAY ESLLAKSLVD VYFDRAIRWL
     SEHSFIREEN NTFTLTNFGK RVADLYINPF TADIIRKGLE GHKASCEIAY LHLLAFTPDG
     PLVSVGRNEE EELIELLEDL ECELLVEEPY EEDEYSLYLN ALKVALIMKD WIDEVDEDTI
     LGKYNIGSGD LRNIVETMDW LTYSAYHLSK ELRLDDHSDK LRILNLRVTD GVKEELLELV
     QIGGVGRKRA RLLYNNGIKG LGDVVMNPDR VRNLLGQKLG ERVVQEAARL LNRFH