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HEM1_DELLE
ID   HEM1_DELLE              Reviewed;         640 AA.
AC   Q9XS79;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=5-aminolevulinate synthase, non-specific, mitochondrial;
DE            Short=ALAS-H;
DE            EC=2.3.1.37 {ECO:0000250|UniProtKB:P13196};
DE   AltName: Full=5-aminolevulinic acid synthase 1;
DE   AltName: Full=Delta-ALA synthase 1;
DE   AltName: Full=Delta-aminolevulinate synthase 1;
DE   Flags: Precursor;
GN   Name=ALAS1; Synonyms=ALS1;
OS   Delphinapterus leucas (Beluga whale).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Odontoceti;
OC   Monodontidae; Delphinapterus.
OX   NCBI_TaxID=9749;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=10425720; DOI=10.1016/s0305-0491(99)00052-8;
RA   Kreiling J.A., Duncan R., Faggart M.A., Cornell N.W.;
RT   "Comparison of the beluga whale (Delphinapterus leucas) expressed genes for
RT   5-aminolevulinate synthase with those in other vertebrates.";
RL   Comp. Biochem. Physiol. 123B:163-174(1999).
CC   -!- FUNCTION: Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent
CC       condensation of succinyl-CoA and glycine to form aminolevulinic acid
CC       (ALA), with CoA and CO2 as by-products. {ECO:0000250|UniProtKB:P13196}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycine + H(+) + succinyl-CoA = 5-aminolevulinate + CO2 + CoA;
CC         Xref=Rhea:RHEA:12921, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:356416; EC=2.3.1.37;
CC         Evidence={ECO:0000250|UniProtKB:P13196};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12922;
CC         Evidence={ECO:0000250|UniProtKB:P13196};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:P13196};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; 5-aminolevulinate from glycine: step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P22557}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P22557}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P22557}. Note=Localizes to the matrix side of
CC       the mitochondrion inner membrane. {ECO:0000250|UniProtKB:P22557}.
CC   -!- SIMILARITY: Belongs to the class-II pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; AF073337; AAD20806.1; -; mRNA.
DR   AlphaFoldDB; Q9XS79; -.
DR   SMR; Q9XS79; -.
DR   STRING; 9749.Q9XS79; -.
DR   PRIDE; Q9XS79; -.
DR   UniPathway; UPA00251; UER00375.
DR   Proteomes; UP000248483; Unplaced.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:InterPro.
DR   GO; GO:0003870; F:5-aminolevulinate synthase activity; ISS:UniProtKB.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:1903412; P:response to bile acid; ISS:UniProtKB.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR010961; 4pyrrol_synth_NH2levulA_synth.
DR   InterPro; IPR015118; 5aminolev_synth_preseq.
DR   InterPro; IPR001917; Aminotrans_II_pyridoxalP_BS.
DR   InterPro; IPR004839; Aminotransferase_I/II.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00155; Aminotran_1_2; 1.
DR   Pfam; PF09029; Preseq_ALAS; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR01821; 5aminolev_synth; 1.
DR   PROSITE; PS00599; AA_TRANSFER_CLASS_2; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Heme biosynthesis; Hydroxylation; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Pyridoxal phosphate; Reference proteome;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..56
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250|UniProtKB:P07997"
FT   CHAIN           57..640
FT                   /note="5-aminolevulinate synthase, non-specific,
FT                   mitochondrial"
FT                   /id="PRO_0000001229"
FT   REGION          60..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        76..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        445
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         217
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         334
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         353
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         386
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         414
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         442
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         474
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         475
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   BINDING         562
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
FT   MOD_RES         445
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250|UniProtKB:P18079"
SQ   SEQUENCE   640 AA;  70620 MW;  357BC41CE8EDBA91 CRC64;
     METVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEVG AKPAPRALSA SAVLCQQVKE
     TPPANEKDKT AKAKVQQAPD GSQQTPDGTQ LPSGHPSLAA SQGTASKCPF LAAQMSQGGS
     SVFCKASLEL QEDVQEMHAV REEVAQTSVN PSVISVKTDG GELSGLLKNF QDIMRKQRPE
     GVSHLLQDNL PKSVSTFQYD HFFEKKIDEK KNDHTYRVFK TVNRRAQFFP MADDYSDSLV
     TKKQVSVWCS NDYLGMSCHP RVCGAVMDTL KQHGTGAGGT RNISGTSKFH VDLEQELADL
     HGKDAALLFS SCFVANDSTL FTLAKMMPGC EIYSDSGNHA SMIQGIWNSR VPKYIYRHND
     VDHLRELLQR SDPAVPKIVA FETVHSMDGA VCPLEELCDV AHEFGAITFV DEVHAVGLYG
     AQGGGIGDRD GVMPKMDIIS GTLGKAFGCV GGYIASTSSL IDTIRSYAAG FIFTTSLPPM
     LLAGALESVR ILKSTEGRVL RRQHQRNVKL MRQMLMDASL PVVHCPSHII PVRVADAAKN
     TEVCNELMSR HNIYVQAINY PTVRRGEELL RIAPTPHHTP QMMNYFVENL LATWKRVGLE
     LKPHSSAECN FCRRPLHFEV MSEREKSYFF GMSKLVSAQD
 
 
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