ANGR_VIBA7
ID ANGR_VIBA7 Reviewed; 1048 AA.
AC Q6W4T3; P19828;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Anguibactin system regulator;
GN Name=angR;
OS Vibrio anguillarum (strain ATCC 68554 / 775) (Listonella anguillarum).
OG Plasmid pJM1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=882102;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=2311935; DOI=10.1016/0378-1119(90)90112-5;
RA Farrell D.H., Mikesell P., Actis L.A., Crosa J.H.;
RT "A regulatory gene, angR, of the iron uptake system of Vibrio anguillarum:
RT similarity with phage P22 cro and regulation by iron.";
RL Gene 86:45-51(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=13129954; DOI=10.1128/jb.185.19.5822-5830.2003;
RA Di Lorenzo M., Stork M., Tolmasky M.E., Actis L.A., Farrell D., Welch T.J.,
RA Crosa L.M., Wertheimer A.M., Chen Q., Salinas P., Waldbeser L., Crosa J.H.;
RT "Complete sequence of virulence plasmid pJM1 from the marine fish pathogen
RT Vibrio anguillarum strain 775.";
RL J. Bacteriol. 185:5822-5830(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=21576332; DOI=10.1128/iai.05138-11;
RA Naka H., Dias G.M., Thompson C.C., Dubay C., Thompson F.L., Crosa J.H.;
RT "Complete genome sequence of the marine fish pathogen Vibrio anguillarum
RT harboring the pJM1 virulence plasmid and genomic comparison with other
RT virulent strains of V. anguillarum and V. ordalii.";
RL Infect. Immun. 79:2889-2900(2011).
RN [4]
RP FUNCTION, PATHWAY, AND MUTAGENESIS OF VAL-151; HIS-267; GLN-303; LYS-609;
RP VAL-852; ILE-859; THR-888 AND LEU-889.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=10569768; DOI=10.1128/iai.67.12.6496-6509.1999;
RA Wertheimer A.M., Verweij W., Chen Q., Crosa L.M., Nagasawa M.,
RA Tolmasky M.E., Actis L.A., Crosa J.H.;
RT "Characterization of the angR gene of Vibrio anguillarum: essential role in
RT virulence.";
RL Infect. Immun. 67:6496-6509(1999).
CC -!- FUNCTION: Bifunctional protein that plays an essential role in
CC virulence. Plays a role in both production of the siderophore
CC anguibactin and regulation of iron transport genes.
CC {ECO:0000269|PubMed:10569768, ECO:0000269|PubMed:2311935}.
CC -!- PATHWAY: Siderophore biosynthesis; anguibactin biosynthesis.
CC {ECO:0000269|PubMed:10569768}.
CC -!- INDUCTION: AngR transcription is regulated by iron.
CC {ECO:0000269|PubMed:2311935}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
CC -!- CAUTION: Lacks the conserved Ser at position 1000 required for covalent
CC attachment of 4-phosphopantetheine. {ECO:0000305}.
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DR EMBL; M34504; AAA79860.1; -; Genomic_DNA.
DR EMBL; AY312585; AAR12528.1; -; Genomic_DNA.
DR PIR; JQ0416; YGVCAR.
DR RefSeq; NP_943553.1; NC_005250.1.
DR RefSeq; WP_011154639.1; NC_005250.1.
DR AlphaFoldDB; Q6W4T3; -.
DR SMR; Q6W4T3; -.
DR eggNOG; COG1020; Bacteria.
DR OMA; WPRYFAG; -.
DR BioCyc; MetaCyc:MON-20471; -.
DR UniPathway; UPA00016; -.
DR Proteomes; UP000006800; Plasmid pJM1.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR Gene3D; 1.10.1200.10; -; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.30.559.10; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR009081; PP-bd_ACP.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00668; Condensation; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Ion transport; Iron; Iron transport; Ligase;
KW Plasmid; Transcription; Transcription regulation; Transport.
FT CHAIN 1..1048
FT /note="Anguibactin system regulator"
FT /id="PRO_0000417119"
FT DOMAIN 965..1039
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MUTAGEN 151
FT /note="V->D: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 267
FT /note="H->L: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 267
FT /note="H->N: Increases anguibactin production. Does not
FT affect ability to grow under iron-limited conditions. No
FT change in virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 267
FT /note="H->Q: Decreases anguibactin production, decreases
FT ability to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 303
FT /note="Q->P: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 609
FT /note="K->F,G: Does not produce anguibactin, impairs
FT ability to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 852
FT /note="V->K: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 859
FT /note="I->P: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 888
FT /note="T->P: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
FT MUTAGEN 889
FT /note="L->P: Does not produce anguibactin, impairs ability
FT to grow under iron-limited conditions and decreases
FT virulence."
FT /evidence="ECO:0000269|PubMed:10569768"
SQ SEQUENCE 1048 AA; 118421 MW; 53CF4B81D25A3CC4 CRC64;
MNQNEHPFAF PETKLPLTSN QNWQLSTQRQ RTEKKSITNF TYQEFDYENI SRDTLERCLT
TIIKHHPIFG AKLSDDFYLH FPSKTHIETF AVNDLSNALK QDIDKQLADT RSAVTKSRSQ
AIISIMFSIL PKNIIRLHVR FNSVVVDNPS VTLFFEQLTQ LLSGSPLSFL NQEQTISAYN
HKVNNELLSV DLESARWNEY ILTLPSSANL PTICEPEKLD ETDITRRCIT LSQRKWQQLV
TVSKKHNVTP EITLASIFST VLSLWGHQKY LMMRFDITKI NDYTGIIGQF TEPLLVGMSG
FEQSFLSLVK NNQKKFEEAY HYDVKVPVFQ CVNKLSNISD SHRYPANITF SSELLNTNHS
KKAVWGCRQS ANTWLSLHAV IEQEQLVLQW DSQDAIFPKD MIKDMLHSYT DLLDLLSQKD
VNWAQPLPTL LPKHQESIRN KINQQGDLEL TKELLHQRFF KNVESTPNAL AIIHGQESLD
YITLASYAKS CAGALTEAGV KSGDRVAVTM NKGIGQIVAV LGILYAGAIY VPVSLDQPQE
RRESIYQGAG INVILINESD SKNSPSNDLF FFLDWQTAIK SEPMRSPQDV APSQPAYIIY
TSGSTGTPKG VVISHQGALN TCIAINRRYQ IGKNDRVLAL SALHFDLSVY DIFGLLSAGG
TIVLVSELER RDPIAWCQAI EEHNVTMWNS VPALFDMLLT YATCFNSIAP SKLRLTMLSG
DWIGLDLPQR YRNYRVDGQF IAMGGATEAS IWSNVFDVEK VPMEWRSIPY GYPLPRQQYR
VVDDLGRDCP DWVAGELWIG GDGIALGYFD DELKTQAQFL HIDGHAWYRT GDMGCYWPDG
TLEFLGRRDK QVKVGGYRIE LGEIEVALNN IPGVQRAVAI AVGNKDKTLA AFIVMDSEQA
PIVTAPLDAE EVQLLLNKQL PNYMVPKRII FLETFPLTAN GKVDHKALTR MTNREKKTSQ
SINKPIITAS EDRVAKIWND VLGPTELYKS SDFFLSGGDA YNAIEVVKRC HKAGYLIKLS
MLYRYSTIEA FAIIMDRCRL APQEEAEL
