ID   ANGT_GENTR              Reviewed;         482 AA.
AC   Q8H0F2;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Anthocyanin 3'-O-beta-glucosyltransferase;
DE            Short=3'GT;
DE            EC=2.4.1.238;
OS   Gentiana triflora (Clustered gentian).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Gentianaceae; Gentianeae; Gentianinae;
OC   Gentiana.
OX   NCBI_TaxID=55190;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 109-130; 182-186; 203-211;
RP   347-365 AND 457-471, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Japonica;
RX   PubMed=12857844; DOI=10.1104/pp.102.018242;
RA   Fukuchi-Mizutani M., Okuhara H., Fukui Y., Nakao M., Katsumoto Y.,
RA   Yonekura-Sakakibara K., Kusumi T., Hase T., Tanaka Y.;
RT   "Biochemical and molecular characterization of a novel UDP-
RT   glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue
RT   anthocyanin biosynthesis, from gentian.";
RL   Plant Physiol. 132:1652-1663(2003).
CC   -!- FUNCTION: Specifically glucosylates the 3'-hydroxy group of delphinidin
CC       3,5-di-O-glucoside to produce gentiodelphin. Shows a strict specificity
CC       for UDP-glucose as donor.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=delphinidin 3,5-bis-O-beta-D-glucoside + UDP-alpha-D-glucose =
CC         delphinidin 3,3',5-tri-O-beta-D-glucoside + H(+) + UDP;
CC         Xref=Rhea:RHEA:35627, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:77838, ChEBI:CHEBI:77844;
CC         EC=2.4.1.238;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=120 uM for delphinidin 3,5-di-O-glucoside
CC         {ECO:0000269|PubMed:12857844};
CC         Vmax=1370 nmol/min/mg enzyme {ECO:0000269|PubMed:12857844};
CC   -!- TISSUE SPECIFICITY: Abundant in petals and barely detected in leaves.
CC       {ECO:0000269|PubMed:12857844}.
CC   -!- DEVELOPMENTAL STAGE: Expressed early in petals development and
CC       decreases in fully opened flowers. {ECO:0000269|PubMed:12857844}.
CC   -!- INDUCTION: Significantly inhibited by Al(3+) and Co(2+), moderately by
CC       Zn(2+) and not by Mg(2+), Ca(2+), Mn(2+), EDAT or EGTA. Competitively
CC       inhibited by UDP. {ECO:0000269|PubMed:12857844}.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AB076697; BAC54092.1; -; mRNA.
DR   AlphaFoldDB; Q8H0F2; -.
DR   SMR; Q8H0F2; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:BAC54092; -.
DR   BRENDA; 2.4.1.238; 2412.
DR   SABIO-RK; Q8H0F2; -.
DR   GO; GO:0033837; F:anthocyanin 3'-O-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosyltransferase; Transferase.
FT   CHAIN           1..482
FT                   /note="Anthocyanin 3'-O-beta-glucosyltransferase"
FT                   /id="PRO_0000310729"
SQ   SEQUENCE   482 AA;  54041 MW;  BDBC12F21C7F7147 CRC64;
     MDQLHVFFFP FLANGHILPT IDMAKLFSSR GVKATLITTH NNSAIFLKAI NRSKILGFDI
     SVLTIKFPSA EFGLPEGYET ADQARSIDMM DEFFRACILL QEPLEELLKE HRPQALVADL
     FFYWANDAAA KFGIPRLLFH GSSSFAMIAA ESVRRNKPYK NLSSDSDPFV VPDIPDKIIL
     TKSQVPTPDE TEENNTHITE MWKNISESEN DCYGVIVNSF YELEPDYVDY CKNVLGRRAW
     HIGPLSLCNN EGEDVAERGK KSDIDAHECL NWLDSKNPDS VVYVCFGSMA NFNAAQLHEL
     AMGLEESGQE FIWVVRTCVD EEDESKWFPD GFEKRVQENN KGLIIKGWAP QVLILEHEAV
     GAFVSHCGWN STLEGICGGV AMVTWPLFAE QFYNEKLMTD ILRTGVSVGS LQWSRVTTSA
     VVVKRESISK AVRRLMAEEE GVDIRNRAKA LKEKAKKAVE GGGSSYSDLS ALLVELSSYP
     HN