ID   HEM3_CHLP8              Reviewed;         312 AA.
AC   P28464; B3QMK1;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 4.
DT   25-MAY-2022, entry version 143.
DE   RecName: Full=Porphobilinogen deaminase;
DE            Short=PBG;
DE            EC=2.5.1.61;
DE   AltName: Full=Hydroxymethylbilane synthase;
DE            Short=HMBS;
DE   AltName: Full=Pre-uroporphyrinogen synthase;
GN   Name=hemC; OrderedLocusNames=Cpar_0737;
OS   Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) (Chlorobium vibrioforme
OS   subsp. thiosulfatophilum).
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobaculum.
OX   NCBI_TaxID=517417;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9058548; DOI=10.1007/s002849900179;
RA   Majumdar D., Wyche J.H.;
RT   "Molecular cloning and nucleotide sequence of the porphobilinogen deaminase
RT   gene, hemC, from Chlorobium vibrioforme.";
RL   Curr. Microbiol. 34:258-263(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 263 / NCIMB 8327;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z., Overmann J.,
RA   Bryant D.A., Richardson P.;
RT   "Complete sequence of Chlorobaculum parvum NCIB 8327.";
RL   Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-85.
RX   PubMed=1793335; DOI=10.1007/bf00262999;
RA   Majumdar D., Avissar Y.J., Wyche J.H., Beale S.I.;
RT   "Structure and expression of the Chlorobium vibrioforme hemA gene.";
RL   Arch. Microbiol. 156:281-289(1991).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+);
CC         Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61;
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342;
CC       Note=Binds 1 dipyrromethane group covalently.;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBUNIT: Monomer.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA23113.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=AAC61857.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M96364; AAA23113.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; CP001099; ACF11154.1; -; Genomic_DNA.
DR   EMBL; AF080069; AAC61857.1; ALT_INIT; Genomic_DNA.
DR   PIR; B48359; B48359.
DR   RefSeq; WP_012501987.1; NC_011027.1.
DR   AlphaFoldDB; P28464; -.
DR   SMR; P28464; -.
DR   STRING; 517417.Cpar_0737; -.
DR   EnsemblBacteria; ACF11154; ACF11154; Cpar_0737.
DR   KEGG; cpc:Cpar_0737; -.
DR   eggNOG; COG0181; Bacteria.
DR   HOGENOM; CLU_019704_0_2_10; -.
DR   OMA; LWQANHI; -.
DR   OrthoDB; 1450400at2; -.
DR   UniPathway; UPA00251; UER00319.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000008811; Chromosome.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   HAMAP; MF_00260; Porphobil_deam; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022419; Porphobilin_deaminase_cofac_BS.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   InterPro; IPR036803; Porphobilinogen_deaminase_C_sf.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 1.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
DR   PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Porphyrin biosynthesis; Transferase.
FT   CHAIN           1..312
FT                   /note="Porphobilinogen deaminase"
FT                   /id="PRO_0000142923"
FT   MOD_RES         241
FT                   /note="S-(dipyrrolylmethanemethyl)cysteine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        36
FT                   /note="T -> S (in Ref. 1; AAA23113 and 3; AAC61857)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   312 AA;  34358 MW;  C7A0792CCAD89EF2 CRC64;
     MKKEIIIGTR SSPLALWQAE FTKAELSKRF PDMNITLKLV KTTGDVLLDS PLSKIGDMGL
     FTKDIEKHLL AGEIDLAVHS LKDVPTGTPE GLVITSFTER EDTRDVIISK SGKGLMDLPQ
     NAKMATSSLR RMSQLLSLRP DLEIMDIRGN LNTRFKKFDE GDFDAMMLAY AGVYRLEFSD
     RITEILPHET MLPAVGQGAL GIETRTDDAE TREIVRVLND DNTEMCCRAE RALLRHLQGG
     CQIPIGSFGS YIDGTLKLLA FVGSVDGKTG LRNEVTKAVK TPEEAEAVGI ELAEILLSMG
     AEKILADIRK TC