ID   HEM3_HALSA              Reviewed;         396 AA.
AC   Q9HMY5;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Probable porphobilinogen deaminase;
DE            Short=PBG;
DE            EC=2.5.1.61;
DE   AltName: Full=Hydroxymethylbilane synthase;
DE            Short=HMBS;
DE   AltName: Full=Pre-uroporphyrinogen synthase;
GN   Name=hemC; Synonyms=hem3; OrderedLocusNames=VNG_2330G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+);
CC         Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61;
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342; Evidence={ECO:0000250};
CC       Note=Binds 1 dipyrromethane group covalently. {ECO:0000250};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000305}.
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DR   EMBL; AE004437; AAG20436.1; -; Genomic_DNA.
DR   PIR; H84383; H84383.
DR   RefSeq; WP_010903738.1; NC_002607.1.
DR   AlphaFoldDB; Q9HMY5; -.
DR   SMR; Q9HMY5; -.
DR   STRING; 64091.VNG_2330G; -.
DR   PaxDb; Q9HMY5; -.
DR   EnsemblBacteria; AAG20436; AAG20436; VNG_2330G.
DR   GeneID; 5953345; -.
DR   KEGG; hal:VNG_2330G; -.
DR   PATRIC; fig|64091.14.peg.1803; -.
DR   HOGENOM; CLU_019704_1_0_2; -.
DR   InParanoid; Q9HMY5; -.
DR   OMA; LWQANHI; -.
DR   OrthoDB; 95523at2157; -.
DR   PhylomeDB; Q9HMY5; -.
DR   UniPathway; UPA00251; UER00319.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IBA:GO_Central.
DR   GO; GO:0006783; P:heme biosynthetic process; IBA:GO_Central.
DR   GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022419; Porphobilin_deaminase_cofac_BS.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   InterPro; IPR036803; Porphobilinogen_deaminase_C_sf.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 2.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
DR   PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1.
PE   3: Inferred from homology;
KW   Porphyrin biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..396
FT                   /note="Probable porphobilinogen deaminase"
FT                   /id="PRO_0000143022"
FT   REGION          159..245
FT                   /note="Insert"
FT   REGION          159..224
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..192
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        197..218
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         328
FT                   /note="S-(dipyrrolylmethanemethyl)cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   396 AA;  42813 MW;  ED1F6692892D04B9 CRC64;
     MSSQQIRLAT RGSDLALRQA GEVVDTLEDR RHDVELVEVE TEGDRVTDAL ISDLGKTGAF
     VRALDQEVME GTVDAAVHSM KDVPTEVPED LVVAAVPHRE NPADVLVTPD GTDLEDLPAG
     SVVGTASLRR GAQVQAHRPG LDVEPIRGNV GTRVEKLLAP ALHREHERRT EAEKEAQSRD
     AREQRRGDYT ADIEAGVENL DTEDGEEGAA DDGDDTASSS EFEQSVTEWF DSLTPLQQSA
     MERDPDTEYD ALVMARVGLE RTGLLHHVGI EELSTGTHVP ATGQGALCVT ARRDSDVVDT
     LRDALEHVRT RVEVTAERVV LEELGGGCIA PIGVHALVQG DTIRTAVQVF SQDGSEQVGE
     TRELDAEQYA TDARELAADL RDRGAADLIE DARTEA