ID   ANIS1_ANISI             Reviewed;         194 AA.
AC   Q7Z1K3; Q7M4A7;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Major allergen Ani s 1;
DE   AltName: Full=21 kDa allergen;
DE   AltName: Full=Excretory gland allergen Ans1;
DE   AltName: Allergen=Ani s 1;
DE   Flags: Precursor;
OS   Anisakis simplex (Herring worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Ascaridomorpha; Ascaridoidea; Anisakidae; Anisakis;
OC   Anisakis simplex complex.
OX   NCBI_TaxID=6269;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Shimakura K., Miura H., Ikeda K., Ishizaki S., Nagashima Y., Shiomi K.,
RA   Shirai T., Kasuya S.;
RT   "Purification and molecular cloning of a majpr allergen from Anisakis
RT   simplex.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 24-40, SUBCELLULAR LOCATION, AND ALLERGEN.
RX   PubMed=10887322; DOI=10.1067/mai.2000.106732;
RA   Moneo I., Caballero M.L., Gomez F., Ortega E., Alonso M.J.;
RT   "Isolation and characterization of a major allergen from the fish parasite
RT   Anisakis simplex.";
RL   J. Allergy Clin. Immunol. 106:177-182(2000).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10887322}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. A.simplex
CC       is a fish parasite that, when accidentally ingested by humans, may
CC       cause allergic reactions in sensitized individuals.
CC       {ECO:0000269|PubMed:10887322}.
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DR   EMBL; AB100095; BAC77154.1; -; mRNA.
DR   PIR; A59069; A59069.
DR   AlphaFoldDB; Q7Z1K3; -.
DR   SMR; Q7Z1K3; -.
DR   Allergome; 3079; Ani s 1.0101.
DR   Allergome; 35; Ani s 1.
DR   MEROPS; I02.968; -.
DR   Proteomes; UP000036680; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR006150; Cys_repeat_1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR028150; Lustrin_cystein.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   Pfam; PF14625; Lustrin_cystein; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SMART; SM00289; WR1; 2.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Secreted; Serine protease inhibitor; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:10887322"
FT   CHAIN           24..194
FT                   /note="Major allergen Ani s 1"
FT                   /id="PRO_0000016888"
FT   DOMAIN          28..84
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DOMAIN          90..134
FT                   /note="WR1"
FT   DISULFID        28..84
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        38..67
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        59..80
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   CONFLICT        37
FT                   /note="P -> Q (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   194 AA;  21185 MW;  F1ADA1A463633B37 CRC64;
     MASMQHFSLA ALLLAASICL GDADRTECQL PLDKGTPCTQ EGGVKPSVAW WHDDKSGICL
     SFKYTGCGGN ANRFTTIKNC EQHCKMPDRG ACALGKKPAE DSNGEQLVCA GMREDKCPNG
     YQCKMMAFMG LCCPTKEEEL FAREYEGVCK SGKPVKMDRG SGWMMTILGK SCDDQFCPED
     AKCERGKLFA NCCK