ID   ANIS6_ANISI             Reviewed;          84 AA.
AC   A1IKL3;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Chymotrypsin inhibitor Ani s 6 {ECO:0000305};
DE   AltName: Allergen=Ani s 6 {ECO:0000303|PubMed:17180690};
DE   Flags: Precursor;
OS   Anisakis simplex (Herring worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Ascaridomorpha; Ascaridoidea; Anisakidae; Anisakis;
OC   Anisakis simplex complex.
OX   NCBI_TaxID=6269 {ECO:0000312|EMBL:BAF43535.1};
RN   [1] {ECO:0000312|EMBL:BAF43535.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND ALLERGEN.
RC   TISSUE=Larva {ECO:0000303|PubMed:17180690};
RX   PubMed=17180690; DOI=10.1007/s00436-006-0396-4;
RA   Kobayashi Y., Ishizaki S., Shimakura K., Nagashima Y., Shiomi K.;
RT   "Molecular cloning and expression of two new allergens from Anisakis
RT   simplex.";
RL   Parasitol. Res. 100:1233-1241(2007).
CC   -!- FUNCTION: Inhibits alpha-chymotrypsin, but not trypsin.
CC       {ECO:0000269|PubMed:17180690}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17180690}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. A.simplex
CC       is a fish parasite that, when accidentally ingested by humans, may
CC       cause allergic reactions in sensitized individuals.
CC       {ECO:0000269|PubMed:17180690}.
CC   -!- SIMILARITY: Belongs to the serine protease inhibitor-like (TIL domain-
CC       containing) family. {ECO:0000305}.
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DR   EMBL; AB274999; BAF43535.1; -; mRNA.
DR   AlphaFoldDB; A1IKL3; -.
DR   SMR; A1IKL3; -.
DR   Allergome; 3767; Ani s 6.
DR   Allergome; 3768; Ani s 6.0101.
DR   Proteomes; UP000036680; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:UniProtKB.
DR   InterPro; IPR036084; Ser_inhib-like_sf.
DR   InterPro; IPR002919; TIL_dom.
DR   Pfam; PF01826; TIL; 1.
DR   SUPFAM; SSF57567; SSF57567; 1.
PE   1: Evidence at protein level;
KW   Allergen; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..84
FT                   /note="Chymotrypsin inhibitor Ani s 6"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002635037"
FT   DOMAIN          25..79
FT                   /note="TIL"
FT                   /evidence="ECO:0000255"
FT   DISULFID        25..58
FT                   /evidence="ECO:0000250|UniProtKB:P83516"
FT   DISULFID        34..54
FT                   /evidence="ECO:0000250|UniProtKB:P83516"
FT   DISULFID        38..50
FT                   /evidence="ECO:0000250|UniProtKB:P83516"
FT   DISULFID        42..79
FT                   /evidence="ECO:0000250|UniProtKB:P83516"
FT   DISULFID        60..73
FT                   /evidence="ECO:0000250|UniProtKB:P83516"
SQ   SEQUENCE   84 AA;  9739 MW;  41F842FCA9FEF5E7 CRC64;
     MFQSTFFLVL MVCVATARFA NKDHCPPNEE YNECGNPCQE KCDNGEPVIC TYQCEHRCFC
     KQGYVRLTED GECVPEEFCK PIHY