3SDC7_OPHHA
ID 3SDC7_OPHHA Reviewed; 84 AA.
AC Q69CK0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Beta-cardiotoxin CTX27;
DE AltName: Full=OH-27;
DE Flags: Precursor;
OS Ophiophagus hannah (King cobra) (Naja hannah).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Ophiophagus.
OX NCBI_TaxID=8665;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=15302536; DOI=10.1016/j.toxicon.2004.06.003;
RA He Y.-Y., Lee W.-H., Zhang Y.;
RT "Cloning and purification of alpha-neurotoxins from king cobra (Ophiophagus
RT hannah).";
RL Toxicon 44:295-303(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-51, FUNCTION, MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom, and Venom gland;
RX PubMed=17616557; DOI=10.1096/fj.07-8658com;
RA Rajagopalan N., Pung Y.F., Zhu Y.Z., Wong P.T.H., Kumar P.P., Kini R.M.;
RT "Beta-cardiotoxin: a new three-finger toxin from Ophiophagus hannah (king
RT cobra) venom with beta-blocker activity.";
RL FASEB J. 21:3685-3695(2007).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=24297900; DOI=10.1073/pnas.1314702110;
RA Vonk F.J., Casewell N.R., Henkel C.V., Heimberg A.M., Jansen H.J.,
RA McCleary R.J., Kerkkamp H.M., Vos R.A., Guerreiro I., Calvete J.J.,
RA Wuster W., Woods A.E., Logan J.M., Harrison R.A., Castoe T.A.,
RA de Koning A.P., Pollock D.D., Yandell M., Calderon D., Renjifo C.,
RA Currier R.B., Salgado D., Pla D., Sanz L., Hyder A.S., Ribeiro J.M.,
RA Arntzen J.W., van den Thillart G.E., Boetzer M., Pirovano W., Dirks R.P.,
RA Spaink H.P., Duboule D., McGlinn E., Kini R.M., Richardson M.K.;
RT "The king cobra genome reveals dynamic gene evolution and adaptation in the
RT snake venom system.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:20651-20656(2013).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 22-84, AND DISULFIDE BONDS.
RA Roy A., Qingxiang S., Kini R.M., Sivaraman J.;
RT "Crystal structure of beta-cardiotoxin, a novel three- cardiotoxin from the
RT venom of Ophiophagus hannah.";
RL Submitted (NOV-2010) to the PDB data bank.
CC -!- FUNCTION: Acts as a beta-blocker by binding to beta-1 and beta-2
CC adrenergic receptors (ADRB1 and ADRB2). It dose-dependently decreases
CC the heart rate (bradycardia), whereas conventional cardiotoxins
CC increases it. At 100 mg/kg, intraperitoneal injection into mice
CC provokes labored breathing, impaired locomotion, lack of response to
CC external stimuli, and death (after 30 min).
CC {ECO:0000269|PubMed:17616557}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17616557}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=7012.42; Mass_error=0.91; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17616557};
CC -!- MISCELLANEOUS: Does not affect blood coagulation and does not show
CC significant hemolytic activity. {ECO:0000269|PubMed:17616557}.
CC -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC residue stands at position 52 (Pro-31 in standard classification).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Aminergic toxin sub-subfamily. {ECO:0000305}.
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DR EMBL; AY596935; AAT97257.1; -; mRNA.
DR EMBL; AY354198; AAR10440.1; -; mRNA.
DR PDB; 3PLC; X-ray; 2.41 A; A/B/C=22-84.
DR PDBsum; 3PLC; -.
DR AlphaFoldDB; Q69CK0; -.
DR SMR; Q69CK0; -.
DR PRIDE; Q69CK0; -.
DR TopDownProteomics; Q69CK0; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003572; Cytotoxin_Cobra.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR Pfam; PF00021; UPAR_LY6; 1.
DR PRINTS; PR00282; CYTOTOXIN.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cardiotoxin; Direct protein sequencing; Disulfide bond;
KW G-protein coupled receptor impairing toxin; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:17616557"
FT CHAIN 22..84
FT /note="Beta-cardiotoxin CTX27"
FT /evidence="ECO:0000305|PubMed:17616557"
FT /id="PRO_0000316089"
FT DISULFID 24..43
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:3PLC"
FT DISULFID 36..61
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:3PLC"
FT DISULFID 65..76
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:3PLC"
FT DISULFID 77..82
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:3PLC"
FT STRAND 23..25
FT /evidence="ECO:0007829|PDB:3PLC"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:3PLC"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:3PLC"
FT STRAND 42..50
FT /evidence="ECO:0007829|PDB:3PLC"
FT STRAND 58..64
FT /evidence="ECO:0007829|PDB:3PLC"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:3PLC"
SQ SEQUENCE 84 AA; 9311 MW; A745B26852DD7085 CRC64;
MKTLLLTLVV VTIVCLDLGY TRKCLNTPLP LIYTTCPIGQ DKCVKMTIKK LPSKYDVIRG
CIDICPKSSA DVEVLCCDTN KCNK
