ID   HEM3_PAEMA              Reviewed;         324 AA.
AC   O69110;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Porphobilinogen deaminase;
DE            Short=PBG;
DE            EC=2.5.1.61;
DE   AltName: Full=Hydroxymethylbilane synthase;
DE            Short=HMBS;
DE   AltName: Full=Pre-uroporphyrinogen synthase;
GN   Name=hemC;
OS   Paenibacillus macerans (Bacillus macerans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=44252;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8244 / DSM 24 / IAM 1227 / JCM 2500 / NBRC 15307 / NCIMB 9368 /
RC   NCTC 6355 / NRRL B-394 / VKM B-506;
RX   PubMed=10217486; DOI=10.1099/13500872-145-3-529;
RA   Johansson P., Hederstedt L.;
RT   "Organization of genes for tetrapyrrole biosynthesis in Gram-positive
RT   bacteria.";
RL   Microbiology 145:529-538(1999).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+);
CC         Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61;
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342; Evidence={ECO:0000250};
CC       Note=Binds 1 dipyrromethane group covalently. {ECO:0000250};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000305}.
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DR   EMBL; AF064061; AAC18587.1; -; Genomic_DNA.
DR   AlphaFoldDB; O69110; -.
DR   SMR; O69110; -.
DR   STRING; 44252.DJ90_5783; -.
DR   UniPathway; UPA00251; UER00319.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   HAMAP; MF_00260; Porphobil_deam; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022419; Porphobilin_deaminase_cofac_BS.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   InterPro; IPR036803; Porphobilinogen_deaminase_C_sf.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 1.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
DR   PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1.
PE   3: Inferred from homology;
KW   Porphyrin biosynthesis; Transferase.
FT   CHAIN           1..324
FT                   /note="Porphobilinogen deaminase"
FT                   /id="PRO_0000142966"
FT   REGION          261..279
FT                   /note="Insert"
FT   MOD_RES         246
FT                   /note="S-(dipyrrolylmethanemethyl)cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   324 AA;  34903 MW;  9F674AA6865B2F85 CRC64;
     MTKRTIVVGT RQSQLALTQT EQVIGDLKEL CRAHGLPFEF EIKKIVTKGD RILDVTLSKI
     GGKGLFVKEI EQAMLDGEID MAVHSMKDMP SVLPEGLING GVPLRKDPRD ALISRSGLHL
     HELPQGARVG TSTLRRSSQL LAYRPDLVLE PVRGNIDSRL RKLEEEGFDA IILAAAGLQR
     MGWENRVTAY LSADVCLPAV GQGALGIECR ENDAELRDVL SLYNDPDTAL TVAAERRFLA
     VLNGGCQVPI AAYANLERAG GQAPEEGGRA AASQAPAALR IRLTGMVGSA DGRGYFEGEC
     RREDPVAFGE RIAGLLLAQG GRVT