ID   HEM3_PEA                Reviewed;         369 AA.
AC   Q43082;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Porphobilinogen deaminase, chloroplastic;
DE            Short=PBG;
DE            EC=2.5.1.61;
DE   AltName: Full=Hydroxymethylbilane synthase;
DE            Short=HMBS;
DE   AltName: Full=Pre-uroporphyrinogen synthase;
DE   Flags: Precursor;
GN   Name=HEMC;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Little Marvel; TISSUE=Leaf;
RX   PubMed=7516080; DOI=10.1104/pp.103.1.139;
RA   Witty M., Wallace-Cook A.D.M., Albrecht H., Spano A.J., Michel H.,
RA   Shabanowitz J., Hunt D.F., Timko M.P., Smith A.G.;
RT   "Structure and expression of chloroplast-localized porphobilinogen
RT   deaminase from pea (Pisum sativum L.) isolated by redundant polymerase
RT   chain reaction.";
RL   Plant Physiol. 103:139-147(1993).
CC   -!- FUNCTION: Tetrapolymerization of the monopyrrole PBG into the
CC       hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+);
CC         Xref=Rhea:RHEA:13185, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:57845, ChEBI:CHEBI:58126; EC=2.5.1.61;
CC   -!- COFACTOR:
CC       Name=dipyrromethane; Xref=ChEBI:CHEBI:60342;
CC       Note=Binds 1 dipyrromethane group covalently.;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: The porphobilinogen subunits are added to the
CC       dipyrromethane group. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HMBS family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X73418; CAA51820.1; -; mRNA.
DR   PIR; S35873; JQ2278.
DR   AlphaFoldDB; Q43082; -.
DR   SMR; Q43082; -.
DR   UniPathway; UPA00251; UER00319.
DR   UniPathway; UPA00668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004418; F:hydroxymethylbilane synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018160; P:peptidyl-pyrromethane cofactor linkage; IEA:InterPro.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.160.40; -; 1.
DR   HAMAP; MF_00260; Porphobil_deam; 1.
DR   InterPro; IPR000860; HemC.
DR   InterPro; IPR022419; Porphobilin_deaminase_cofac_BS.
DR   InterPro; IPR022417; Porphobilin_deaminase_N.
DR   InterPro; IPR022418; Porphobilinogen_deaminase_C.
DR   InterPro; IPR036803; Porphobilinogen_deaminase_C_sf.
DR   PANTHER; PTHR11557; PTHR11557; 1.
DR   Pfam; PF01379; Porphobil_deam; 1.
DR   Pfam; PF03900; Porphobil_deamC; 1.
DR   PIRSF; PIRSF001438; 4pyrrol_synth_OHMeBilane_synth; 1.
DR   PRINTS; PR00151; PORPHBDMNASE.
DR   SUPFAM; SSF54782; SSF54782; 1.
DR   TIGRFAMs; TIGR00212; hemC; 1.
DR   PROSITE; PS00533; PORPHOBILINOGEN_DEAM; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll biosynthesis; Chloroplast; Direct protein sequencing; Plastid;
KW   Porphyrin biosynthesis; Transferase; Transit peptide.
FT   TRANSIT         1..46
FT                   /note="Chloroplast"
FT   CHAIN           47..369
FT                   /note="Porphobilinogen deaminase, chloroplastic"
FT                   /id="PRO_0000013323"
FT   MOD_RES         303
FT                   /note="S-(dipyrrolylmethanemethyl)cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   369 AA;  39931 MW;  F7660D8390894431 CRC64;
     MEMTLYSSSS FSLPSAPSNP SLSLFTSSFR FSSFKTSPFS KCRIRASLAV EQQTQQNKTA
     LIRIGTRGSP LALAQAHETR DKLMASHTEL AEEGAIQIVI IKTTGDKILS QPLADIGGKG
     LFTKEIDEAL INGDIDIAVH SMKDVPTYLP EETILPCNLP REDVRDAFIS LSAASLADLP
     AGSVIGTASL RRKSQILHRY PSLTVQDNFR GNVQTRLRKL SEGVVKATLL ALAGLKRLNM
     TENVTSTLSI DDMLPAVAQG AIGIACRSND DKMAEYLASL NHEETRLAIS CERAFLTTLD
     GSCRTPIAGY ASRDKDGNCL FRGLVASPDG TRVLETSRIG SYTYEDMMKI GKDAGEELLS
     RAGPGFFNS