HEM4_ECOLI
ID HEM4_ECOLI Reviewed; 246 AA.
AC P09126; Q2M8A9; Q47250;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Uroporphyrinogen-III synthase;
DE Short=UROS;
DE EC=4.2.1.75;
DE AltName: Full=Hydroxymethylbilane hydrolyase [cyclizing];
DE AltName: Full=Uroporphyrinogen-III cosynthase;
GN Name=hemD; OrderedLocusNames=b3804, JW3776;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / CS520;
RX PubMed=3054815; DOI=10.1093/nar/16.20.9871;
RA Alefounder P.R., Abell C., Battersby A.R.;
RT "The sequence of hemC, hemD and two additional E. coli genes.";
RL Nucleic Acids Res. 16:9871-9871(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3320969; DOI=10.1093/nar/15.24.10583;
RA Jordan P.M., Mgbeje B.I.A., Alwan A.F., Thomas S.D.;
RT "Nucleotide sequence of hemD, the second gene in the hem operon of
RT Escherichia coli K-12.";
RL Nucleic Acids Res. 15:10583-10583(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3277628; DOI=10.1042/bj2490613;
RA Jordan P.M., Mgbeje B.I.A., Thomas S.D., Alwan A.F.;
RT "Nucleotide sequence for the hemD gene of Escherichia coli encoding
RT uroporphyrinogen III synthase and initial evidence for a hem operon.";
RL Biochem. J. 249:613-616(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3040684; DOI=10.1128/jb.169.9.4257-4262.1987;
RA Sasarman A., Nepveu A., Echelard Y., Dymetryszyn J., Drolet M., Goyer C.;
RT "Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12
RT and preliminary data on the Uro operon.";
RL J. Bacteriol. 169:4257-4262(1987).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Catalyzes cyclization of the linear tetrapyrrole,
CC hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydroxymethylbilane = H2O + uroporphyrinogen III;
CC Xref=Rhea:RHEA:18965, ChEBI:CHEBI:15377, ChEBI:CHEBI:57308,
CC ChEBI:CHEBI:57845; EC=4.2.1.75;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
CC -!- SUBUNIT: Monomer.
CC -!- SIMILARITY: Belongs to the uroporphyrinogen-III synthase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X12614; CAA31133.1; -; Genomic_DNA.
DR EMBL; Y00883; CAA68776.1; -; Genomic_DNA.
DR EMBL; M17360; AAA23956.1; -; Genomic_DNA.
DR EMBL; M87049; AAA67600.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76807.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77497.1; -; Genomic_DNA.
DR PIR; E65184; E65184.
DR RefSeq; NP_418248.1; NC_000913.3.
DR RefSeq; WP_000026046.1; NZ_SSZK01000025.1.
DR AlphaFoldDB; P09126; -.
DR SMR; P09126; -.
DR BioGRID; 4262609; 4.
DR IntAct; P09126; 3.
DR STRING; 511145.b3804; -.
DR jPOST; P09126; -.
DR PaxDb; P09126; -.
DR PRIDE; P09126; -.
DR DNASU; 948587; -.
DR EnsemblBacteria; AAC76807; AAC76807; b3804.
DR EnsemblBacteria; BAE77497; BAE77497; BAE77497.
DR GeneID; 948587; -.
DR KEGG; ecj:JW3776; -.
DR KEGG; eco:b3804; -.
DR PATRIC; fig|1411691.4.peg.2904; -.
DR EchoBASE; EB0425; -.
DR eggNOG; COG1587; Bacteria.
DR HOGENOM; CLU_011276_9_4_6; -.
DR InParanoid; P09126; -.
DR OMA; HPRIAQA; -.
DR PhylomeDB; P09126; -.
DR BioCyc; EcoCyc:UROGENIIISYN-MON; -.
DR BioCyc; MetaCyc:UROGENIIISYN-MON; -.
DR UniPathway; UPA00251; UER00320.
DR PRO; PR:P09126; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0004852; F:uroporphyrinogen-III synthase activity; IDA:EcoCyc.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IDA:EcoCyc.
DR GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IEA:InterPro.
DR CDD; cd06578; HemD; 1.
DR Gene3D; 3.40.50.10090; -; 2.
DR InterPro; IPR036108; 4pyrrol_syn_uPrphyn_synt_sf.
DR InterPro; IPR003754; 4pyrrol_synth_uPrphyn_synth.
DR InterPro; IPR039793; UROS/Hem4.
DR PANTHER; PTHR38042; PTHR38042; 1.
DR Pfam; PF02602; HEM4; 1.
DR SUPFAM; SSF69618; SSF69618; 1.
PE 3: Inferred from homology;
KW Lyase; Porphyrin biosynthesis; Reference proteome.
FT CHAIN 1..246
FT /note="Uroporphyrinogen-III synthase"
FT /id="PRO_0000135243"
FT CONFLICT 146
FT /note="T -> A (in Ref. 4; AAA23956)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="A -> R (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 246 AA; 27798 MW; 0BF7453C72395A0C CRC64;
MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL GESDLLFALS
QHAVAFAQSQ LHQQDRKWPR LPDYFAIGRT TALALHTVSG QKILYPQDRE ISEVLLQLPE
LQNIAGKRAL ILRGNGGREL IGDTLTARGA EVTFCECYQR CAIHYDGAEE AMRWQAREVT
MVVVTSGEML QQLWSLIPQW YREHWLLHCR LLVVSERLAK LARELGWQDI KVADNADNDA
LLRALQ
