HEM4_ORYSJ
ID HEM4_ORYSJ Reviewed; 302 AA.
AC Q10QR9; A0A0P0VU16;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Uroporphyrinogen-III synthase, chloroplastic;
DE EC=4.2.1.75;
DE AltName: Full=Hydroxymethylbilane hydrolyase [cyclizing];
DE AltName: Full=Uroporphyrinogen-III cosynthase;
DE Flags: Precursor;
GN Name=UROS; Synonyms=HEMD; OrderedLocusNames=Os03g0186100, LOC_Os03g08730;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes cyclization of the linear tetrapyrrole,
CC hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a
CC precursor of tetrapyrroles such as chlorophyll, heme and phycobilins.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydroxymethylbilane = H2O + uroporphyrinogen III;
CC Xref=Rhea:RHEA:18965, ChEBI:CHEBI:15377, ChEBI:CHEBI:57308,
CC ChEBI:CHEBI:57845; EC=4.2.1.75;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the uroporphyrinogen-III synthase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DP000009; ABF94358.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11115.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82675.1; -; Genomic_DNA.
DR EMBL; AK107127; BAG97962.1; -; mRNA.
DR RefSeq; XP_015630608.1; XM_015775122.1.
DR AlphaFoldDB; Q10QR9; -.
DR SMR; Q10QR9; -.
DR STRING; 4530.OS03T0186100-01; -.
DR PaxDb; Q10QR9; -.
DR PRIDE; Q10QR9; -.
DR EnsemblPlants; Os03t0186100-01; Os03t0186100-01; Os03g0186100.
DR GeneID; 4331869; -.
DR Gramene; Os03t0186100-01; Os03t0186100-01; Os03g0186100.
DR KEGG; osa:4331869; -.
DR eggNOG; ENOG502QST9; Eukaryota.
DR HOGENOM; CLU_011276_9_2_1; -.
DR InParanoid; Q10QR9; -.
DR OMA; LRNVYYP; -.
DR OrthoDB; 1423947at2759; -.
DR PlantReactome; R-OSA-4827054; Tetrapyrrole biosynthesis I.
DR UniPathway; UPA00251; UER00320.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10QR9; OS.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0004852; F:uroporphyrinogen-III synthase activity; IBA:GO_Central.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IBA:GO_Central.
DR CDD; cd06578; HemD; 1.
DR Gene3D; 3.40.50.10090; -; 2.
DR InterPro; IPR036108; 4pyrrol_syn_uPrphyn_synt_sf.
DR InterPro; IPR003754; 4pyrrol_synth_uPrphyn_synth.
DR InterPro; IPR039793; UROS/Hem4.
DR PANTHER; PTHR38042; PTHR38042; 1.
DR Pfam; PF02602; HEM4; 1.
DR SUPFAM; SSF69618; SSF69618; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; Lyase; Plastid;
KW Porphyrin biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..302
FT /note="Uroporphyrinogen-III synthase, chloroplastic"
FT /id="PRO_0000376068"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 302 AA; 32123 MW; E9964659A861998B CRC64;
MALSSSSHLL PFSRPPATFP RARHAGGGRG RAGATGRFIA CSSPPPPDVV VTRERGKNAK
LIAALEKHNV QSLELPLIKH VEGPDTDRLS AVLRDEKFDW ITITSPEAAA VFLEGWKAAG
NPKVRIAVVG AGTERVFDEV IQYNDGSLEV AFSPSKAMGK FLASELPRTT ETTCKVLYPA
SAKAGHEIQN GLSNRGFEVT RLNTYTTVSV QDVDPLILKP ALSAPVVAVA SPSALRAWLN
LASQVDNWGN AIACIGETTA SAAKKFGLKS IYYPTTPGLD GWVESILEAL RAHGQSKEAP
GC
