HEM4_PSEAE
ID HEM4_PSEAE Reviewed; 251 AA.
AC P48246;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Uroporphyrinogen-III synthase;
DE Short=UROS;
DE EC=4.2.1.75;
DE AltName: Full=Hydroxymethylbilane hydrolyase [cyclizing];
DE AltName: Full=Uroporphyrinogen-III cosynthase;
GN Name=hemD; OrderedLocusNames=PA5259;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8159168; DOI=10.1007/bf00391011;
RA Mohr C.D., Sonsteby S.K., Deretic V.;
RT "The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the
RT gene encoding the regulator of mucoidy AlgR.";
RL Mol. Gen. Genet. 242:177-184(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS) OF APOPROTEIN, AND SUBUNIT.
RX PubMed=23295481; DOI=10.1107/s1744309112044739;
RA Moynie L., Schnell R., McMahon S.A., Sandalova T., Boulkerou W.A.,
RA Schmidberger J.W., Alphey M., Cukier C., Duthie F., Kopec J., Liu H.,
RA Jacewicz A., Hunter W.N., Naismith J.H., Schneider G.;
RT "The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic
RT studies for assessment of potential targets in early-stage drug
RT discovery.";
RL Acta Crystallogr. F 69:25-34(2013).
CC -!- FUNCTION: Catalyzes cyclization of the linear tetrapyrrole,
CC hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydroxymethylbilane = H2O + uroporphyrinogen III;
CC Xref=Rhea:RHEA:18965, ChEBI:CHEBI:15377, ChEBI:CHEBI:57308,
CC ChEBI:CHEBI:57845; EC=4.2.1.75;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:23295481}.
CC -!- SIMILARITY: Belongs to the uroporphyrinogen-III synthase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M74844; AAA18908.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG08644.1; -; Genomic_DNA.
DR PIR; A82989; A82989.
DR PIR; S41587; S41587.
DR RefSeq; NP_253946.1; NC_002516.2.
DR RefSeq; WP_003114032.1; NZ_QZGE01000002.1.
DR PDB; 4ES6; X-ray; 2.22 A; A=1-251.
DR PDB; 6TH8; NMR; -; A=130-251.
DR PDBsum; 4ES6; -.
DR PDBsum; 6TH8; -.
DR AlphaFoldDB; P48246; -.
DR BMRB; P48246; -.
DR SMR; P48246; -.
DR STRING; 287.DR97_2630; -.
DR PaxDb; P48246; -.
DR PRIDE; P48246; -.
DR DNASU; 879724; -.
DR EnsemblBacteria; AAG08644; AAG08644; PA5259.
DR GeneID; 879724; -.
DR KEGG; pae:PA5259; -.
DR PATRIC; fig|208964.12.peg.5512; -.
DR PseudoCAP; PA5259; -.
DR HOGENOM; CLU_011276_9_4_6; -.
DR InParanoid; P48246; -.
DR OMA; HPRIAQA; -.
DR PhylomeDB; P48246; -.
DR BioCyc; PAER208964:G1FZ6-5380-MON; -.
DR UniPathway; UPA00251; UER00320.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0004852; F:uroporphyrinogen-III synthase activity; ISS:PseudoCAP.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IEA:InterPro.
DR CDD; cd06578; HemD; 1.
DR Gene3D; 3.40.50.10090; -; 2.
DR InterPro; IPR036108; 4pyrrol_syn_uPrphyn_synt_sf.
DR InterPro; IPR003754; 4pyrrol_synth_uPrphyn_synth.
DR InterPro; IPR039793; UROS/Hem4.
DR PANTHER; PTHR38042; PTHR38042; 1.
DR Pfam; PF02602; HEM4; 1.
DR SUPFAM; SSF69618; SSF69618; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Porphyrin biosynthesis; Reference proteome.
FT CHAIN 1..251
FT /note="Uroporphyrinogen-III synthase"
FT /id="PRO_0000135245"
FT CONFLICT 195..203
FT /note="QNLYQLAAA -> PKSVSVGGS (in Ref. 1; AAA18908)"
FT /evidence="ECO:0000305"
FT STRAND 5..8
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 12..24
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 28..31
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 36..39
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 44..51
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 53..55
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 64..77
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 85..90
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 91..100
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 113..117
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 120..125
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 132..137
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 144..151
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 155..160
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 162..165
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 173..180
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 191..201
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 202..204
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 205..208
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 218..226
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 230..234
FT /evidence="ECO:0007829|PDB:4ES6"
FT STRAND 236..239
FT /evidence="ECO:0007829|PDB:4ES6"
FT HELIX 240..249
FT /evidence="ECO:0007829|PDB:4ES6"
SQ SEQUENCE 251 AA; 27279 MW; EEF61A160175264A CRC64;
MSGWRLLLTR PDEECAALAA SLGEAGVHSS SLPLLAIDPL EETPEQRTLM LDLDRYCAVV
VVSKPAARLG LERLDRYWPQ PPQQTWCSVG AATAAILEAY GLDVTYPEQG DDSEALLALP
AFQDSLRVHD PKVLIMRGEG GREFLAERLR GQGVQVDYLP LYRRRAPDYP AGELLARVRA
ERLNGLVVSS GQGLQNLYQL AAADWPEIGR LPLFVPSPRV AEMARELGAQ RVIDCRGASA
PALLAALTSA A
