HEM62_ARATH
ID HEM62_ARATH Reviewed; 233 AA.
AC Q93Z96; F4JI66; Q710F7;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Coproporphyrinogen-III oxidase 2, chloroplastic;
DE Short=AtCPO-II;
DE Short=Coprogen oxidase;
DE Short=Coproporphyrinogenase;
DE EC=1.3.3.3;
DE Flags: Precursor;
GN Name=CPX2; Synonyms=HEMF2; OrderedLocusNames=At4g03205; ORFNames=F4C21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Landsberg erecta;
RA Santana M.A., Tan F.C., Smith A.G.;
RT "Molecular characterisation of coproporphyrinogen oxidase from Glycine max
RT and Arabidopsis thaliana.";
RL Plant Physiol. Biochem. 40:289-298(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Key enzyme in heme biosynthesis. Catalyzes the oxidative
CC decarboxylation of propionic acid side chains of rings A and B of
CC coproporphyrinogen III (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC route): step 1/1.
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93Z96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93Z96-2; Sequence=VSP_046551;
CC -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD12662.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ420797; CAD12662.1; ALT_FRAME; mRNA.
DR EMBL; CP002687; AEE82290.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82291.1; -; Genomic_DNA.
DR EMBL; AY057708; AAL15338.1; -; mRNA.
DR EMBL; BT006346; AAP21154.1; -; mRNA.
DR RefSeq; NP_567256.3; NM_116557.4. [Q93Z96-1]
DR RefSeq; NP_567257.2; NM_116558.2. [Q93Z96-2]
DR AlphaFoldDB; Q93Z96; -.
DR SMR; Q93Z96; -.
DR BioGRID; 13320; 16.
DR STRING; 3702.AT4G03205.2; -.
DR PaxDb; Q93Z96; -.
DR PRIDE; Q93Z96; -.
DR ProMEX; Q93Z96; -.
DR EnsemblPlants; AT4G03205.1; AT4G03205.1; AT4G03205. [Q93Z96-1]
DR EnsemblPlants; AT4G03205.2; AT4G03205.2; AT4G03205. [Q93Z96-2]
DR GeneID; 828029; -.
DR Gramene; AT4G03205.1; AT4G03205.1; AT4G03205. [Q93Z96-1]
DR Gramene; AT4G03205.2; AT4G03205.2; AT4G03205. [Q93Z96-2]
DR KEGG; ath:AT4G03205; -.
DR Araport; AT4G03205; -.
DR TAIR; locus:505006431; AT4G03205.
DR eggNOG; KOG1518; Eukaryota.
DR HOGENOM; CLU_103932_0_0_1; -.
DR OrthoDB; 1080991at2759; -.
DR PhylomeDB; Q93Z96; -.
DR BRENDA; 1.3.3.3; 399.
DR UniPathway; UPA00251; UER00322.
DR UniPathway; UPA00668; -.
DR PRO; PR:Q93Z96; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93Z96; baseline and differential.
DR Genevisible; Q93Z96; AT.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; IBA:GO_Central.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.1500.10; -; 1.
DR InterPro; IPR001260; Coprogen_oxidase_aer.
DR InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR PANTHER; PTHR10755; PTHR10755; 1.
DR Pfam; PF01218; Coprogen_oxidas; 1.
DR PRINTS; PR00073; COPRGNOXDASE.
DR SUPFAM; SSF102886; SSF102886; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chlorophyll biosynthesis; Chloroplast;
KW Heme biosynthesis; Oxidoreductase; Plastid; Porphyrin biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..233
FT /note="Coproporphyrinogen-III oxidase 2, chloroplastic"
FT /id="PRO_0000422670"
FT ACT_SITE 188
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VAR_SEQ 233
FT /note="V -> VVKPDPLF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046551"
FT CONFLICT 13
FT /note="S -> C (in Ref. 1; CAD12662)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="R -> K (in Ref. 1; CAD12662)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="G -> A (in Ref. 1; CAD12662)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 233 AA; 26134 MW; C2FEA0764B92B42F CRC64;
MASHSSTLFT SPSSFILFSS HRLKSSPNYF TYHFPRSVKR PHFDLRCSVS IEKEVPETER
PFTFLRVSDG DQTQSSSYSV RARFEKMIRT AQDKVCEAIE AVEEGPKFKE DVWSRPGGGG
GISRILQDGN VWEKAGVNVS VIYGVMPPEA YRAAKAATSE QKPGPIPFFA AGTSSVLHPQ
NPFAPTLHFN YRYFETDAPK DVPGAPRQWW FGGGTDFTPA YIFEEDVKHF HSV
