ANKL1_CAEEL
ID ANKL1_CAEEL Reviewed; 704 AA.
AC G5EGA3;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 2.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Ankyrin repeat and LEM domain-containing protein 1 homolog {ECO:0000305};
DE EC=3.1.-.- {ECO:0000269|PubMed:22383942};
DE AltName: Full=LEM-domain containing protein 3 {ECO:0000250|UniProtKB:Q8NAG6};
GN Name=lem-3 {ECO:0000312|WormBase:F42H11.2};
GN Synonyms=rad-1 {ECO:0000312|WormBase:F42H11.2};
GN ORFNames=F42H11.2 {ECO:0000312|WormBase:F42H11.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF LEU-659.
RX PubMed=22383942; DOI=10.1371/journal.pone.0024555;
RA Dittrich C.M., Kratz K., Sendoel A., Gruenbaum Y., Jiricny J.,
RA Hengartner M.O.;
RT "LEM-3 - a LEM domain containing nuclease involved in the DNA damage
RT response in C. elegans.";
RL PLoS ONE 7:E24555-E24555(2012).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, AND MUTAGENESIS OF
RP 141-PHE--VAL-250; 190-ARG--LYS-704; SER-192; SER-194; TYR-556 AND GLY-558.
RX PubMed=29463814; DOI=10.1038/s41467-018-03135-w;
RA Hong Y., Sonneville R., Wang B., Scheidt V., Meier B., Woglar A.,
RA Demetriou S., Labib K., Jantsch V., Gartner A.;
RT "LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges
RT during late mitosis.";
RL Nat. Commun. 9:728-728(2018).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF 190-ARG--LYS-704.
RX PubMed=29879106; DOI=10.1371/journal.pgen.1007453;
RA Hong Y., Velkova M., Silva N., Jagut M., Scheidt V., Labib K., Jantsch V.,
RA Gartner A.;
RT "The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial
RT regulation of meiotic recombination repair and chromosome segregation in
RT Caenorhabditis elegans.";
RL PLoS Genet. 14:E1007453-E1007453(2018).
CC -!- FUNCTION: Endonuclease which, in association with baf-1, plays an
CC essential role during embryogenesis in the DNA repair response
CC following DNA damage probably by ensuring proper chromosome segregation
CC (PubMed:22383942). Also required during postembryonic cell divisions
CC after DNA damage caused by ionizing radiation to ensure normal cell
CC proliferation (PubMed:22383942). Resolves chromatin bridges in late
CC mitosis that result from incomplete DNA replication, defective
CC chromosome condensation or unresolved recombination intermediates
CC (PubMed:29463814). Together with brc-1, contributes to genome integrity
CC by resolving mitotic chromatin bridges that result from incomplete
CC processing of DNA breaks (PubMed:29463814). In parallel to the slx-
CC 1/mus-81 pathway, acts in processing early recombination intermediates
CC in meiotic prophase I to prevent illegitimate recombination
CC (PubMed:29879106). Also involved in processing remaining, erroneous
CC recombination intermediates that persist into the second meiotic
CC division (PubMed:29879106). {ECO:0000269|PubMed:22383942,
CC ECO:0000269|PubMed:29463814, ECO:0000269|PubMed:29879106}.
CC -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:22383942}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22383942,
CC ECO:0000269|PubMed:29463814, ECO:0000269|PubMed:29879106}. Nucleus
CC {ECO:0000269|PubMed:29879106}. Chromosome
CC {ECO:0000269|PubMed:29463814}. Midbody {ECO:0000269|PubMed:29463814}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:29463814}.
CC Note=Localizes to foci-like structures outside the nucleus
CC (PubMed:22383942, PubMed:29463814, PubMed:29879106). In pachytene,
CC localizes to foci both in and outside of the nucleus (PubMed:29879106).
CC Colocalizes with air-2 between dividing nuclei in meiosis II
CC (PubMed:29879106). In mitosis, localizes to the center of chromatin
CC bridges that are formed in response to DNA defects (PubMed:29463814).
CC Forms initially a ring like structure encircling chromatin bridges
CC between two separated daughter cells until in late telophase, localizes
CC to the center of chromatin bridges (PubMed:29463814). Colocalizes with
CC air-2 at the midbody (PubMed:29463814). Midbody localization depends on
CC the formation of the central spindle and the midbody and on air-2
CC (PubMed:29463814). Colocalizes with the central spindle component zen-4
CC at the central spindle (PubMed:29463814). {ECO:0000269|PubMed:22383942,
CC ECO:0000269|PubMed:29463814, ECO:0000269|PubMed:29879106}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:22383942, ECO:0000269|PubMed:29463814}.
CC -!- PTM: Phosphorylated. Phosphorylated during telophase when localized at
CC the midbody. {ECO:0000269|PubMed:29463814}.
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DR EMBL; BX284601; CAB05722.2; -; Genomic_DNA.
DR RefSeq; NP_492539.2; NM_060138.3.
DR AlphaFoldDB; G5EGA3; -.
DR SMR; G5EGA3; -.
DR STRING; 6239.F42H11.2; -.
DR EPD; G5EGA3; -.
DR PaxDb; G5EGA3; -.
DR PeptideAtlas; G5EGA3; -.
DR PRIDE; G5EGA3; -.
DR EnsemblMetazoa; F42H11.2.1; F42H11.2.1; WBGene00002276.
DR GeneID; 172792; -.
DR KEGG; cel:CELE_F42H11.2; -.
DR CTD; 172792; -.
DR WormBase; F42H11.2; CE47022; WBGene00002276; lem-3.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000168304; -.
DR HOGENOM; CLU_391936_0_0_1; -.
DR InParanoid; G5EGA3; -.
DR OMA; CYILIDP; -.
DR OrthoDB; 487558at2759; -.
DR PRO; PR:G5EGA3; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00002276; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000793; C:condensed chromosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:1990023; C:mitotic spindle midzone; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0051233; C:spindle midzone; IDA:UniProtKB.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IDA:WormBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IDA:UniProtKB.
DR GO; GO:0072711; P:cellular response to hydroxyurea; IDA:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; IGI:UniProtKB.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IDA:WormBase.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IGI:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:UniProtKB.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IGI:UniProtKB.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IGI:UniProtKB.
DR GO; GO:0009411; P:response to UV; IMP:WormBase.
DR GO; GO:0010225; P:response to UV-C; IMP:WormBase.
DR GO; GO:0010165; P:response to X-ray; IMP:WormBase.
DR Gene3D; 1.10.720.40; -; 1.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR034998; ANKLE1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR000305; GIY-YIG_endonuc.
DR InterPro; IPR011015; LEM/LEM-like_dom_sf.
DR InterPro; IPR003887; LEM_dom.
DR PANTHER; PTHR46427; PTHR46427; 1.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF03020; LEM; 1.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00540; LEM; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF63451; SSF63451; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50164; GIY_YIG; 1.
DR PROSITE; PS50954; LEM; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cell cycle; Cell division; Chromosome; Cytoplasm; Cytoskeleton;
KW DNA damage; DNA repair; Endonuclease; Hydrolase; Meiosis; Mitosis;
KW Nuclease; Nucleus; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..704
FT /note="Ankyrin repeat and LEM domain-containing protein 1
FT homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438147"
FT REPEAT 28..59
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 63..93
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT DOMAIN 425..470
FT /note="LEM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00313"
FT DOMAIN 525..635
FT /note="GIY-YIG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00977"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 247..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..358
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 381..405
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..421
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 141..250
FT /note="Missing: In tm3468; lack of phosphorylation."
FT /evidence="ECO:0000269|PubMed:29463814"
FT MUTAGEN 190..704
FT /note="Missing: In mn155; increase of rad-51 foci in
FT meiotic prophase and increase in rmh-1 foci in mid
FT pachytene. In a RNAi-mediated mcm-7 or capg-1 knockdown,
FT unresolved chromatin bridges at the end of cell division
FT and reduced embryonic viability."
FT /evidence="ECO:0000269|PubMed:29463814,
FT ECO:0000269|PubMed:29879106"
FT MUTAGEN 192
FT /note="S->A: Increased sensitivity to ionizing radiation,
FT decreased localization to the midbody and lack of
FT phosphorylation; when associated with Ala-194. In a RNAi-
FT mediated capg-1 knockdown, delayed midbody localization and
FT persistent chromatin bridge formation; when associated with
FT Ala-194."
FT /evidence="ECO:0000269|PubMed:29463814"
FT MUTAGEN 194
FT /note="S->A: Increased sensitivity to ionizing radiation,
FT decreased localization to the midbody and lack of
FT phosphorylation; when associated with Ala-192. In a RNAi-
FT mediated capg-1 knockdown, delayed midbody localization and
FT persistent chromatin bridge formation; when associated with
FT Ala-192."
FT /evidence="ECO:0000269|PubMed:29463814"
FT MUTAGEN 556
FT /note="Y->A: Failure to localize to midbodies and
FT hypersensitivity to ionizing radiation; when associated
FT with Ala-558. In a RNAi-mediated capg-1 knockdown,
FT excessive and persistent chromatin-bridge formation; when
FT associated with Ala-558."
FT /evidence="ECO:0000269|PubMed:29463814"
FT MUTAGEN 558
FT /note="G->A: Failure to localize to midbodies and
FT hypersensitivity to ionizing radiation; when associated
FT with Ala-556. In a RNAi-mediated capg-1 knockdown,
FT excessive and persistent chromatin-bridge formation; when
FT associated with Ala-556."
FT /evidence="ECO:0000269|PubMed:29463814"
FT MUTAGEN 659
FT /note="L->F: In op444; severe reduction in endonucleolytic
FT activity. Severe embryonic lethality of progeny of adults
FT treated with X-ray, UV-C light or cisplatin characterized
FT by chromosome mis-segregation and formation of anaphase
FT bridges. Irradiated L1 larvae are uncoordinated and have a
FT protruding vulva. In germline cells, cell cycle arrest and
FT apoptosis following DNA damage are not affected. In
FT addition, progeny of non-irradiated adults die at the
FT embryonic stage in a baf-1 and unc-32 mutant background."
FT /evidence="ECO:0000269|PubMed:22383942"
SQ SEQUENCE 704 AA; 78240 MW; 1B108403842BA56D CRC64;
MPPNGAITTT PRSRMPPTTP SSGKSRPKKE TLHYLAASSS TTSVDAARTL LERGANVNAI
DRDGATPLHY ACTHDNVAMA QLLLTFGADP MSADKLGRTA YSIAKGNTLR FLRRYKKSSN
RQRLGFFRRF FACHSRNETF FIVRNNQEVA PLRPTALAEA ASISFNRGNV LTNSYRCAKK
KIRATFHAIR RSRSNSTATL QDVVLTSEGI RTVTTPSRRA PKATVYAKRS MSVSDLLLIP
DRRDIKNEDV KTRGSPVKKT RGTGRSRTPE AILNPRKQRT PVNHHKRSKS QETKLVAMPS
PNSMAYYNTS RARNAGLRPA PSAPPLSPEP AIAAVKTPKT PTTPKTSKGR SKSPANTTAY
FTADESLELL GNNMEKLNIE SKSAKSSKLK TPSKPTTSSS TSFSSAEDDK EAEVSTPTTV
DDGEIRKIRR LREGELKSEL KKFGISPAGP LDARTRRLYE KKLLIERRKI TNRGYSPDAD
VVSCRNSPQL ELVLRNGFLP ADFASRARKC DENVRSEFSG NGFGYNAFCY LIMDPRILGS
NVENLTLETF VRSIFYVGKG SKNRPLAHFI DARNERRDKL DKLKTCEKLK TIDELWTLGF
GIPRHEISHG VSDEEAFVRE ASIIEAVKLK NLRNKKAGEF HGTTKSWDNI TKSEYGTFLL
DRALSTLKLE GIRLITEDNL PDSLYPYVNN RRGAGGGRTP KTPK