HEM6_DICDI
ID HEM6_DICDI Reviewed; 332 AA.
AC Q54IA7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase;
DE Short=Coprogen oxidase;
DE Short=Coproporphyrinogenase;
DE EC=1.3.3.3;
GN Name=cpox; Synonyms=hemF; ORFNames=DDB_G0288893;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in the heme biosynthesis. Catalyzes the aerobic
CC oxidative decarboxylation of propionate groups of rings A and B of
CC coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-
CC IX (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC route): step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC family. {ECO:0000305}.
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DR EMBL; AAFI02000126; EAL62970.1; -; Genomic_DNA.
DR RefSeq; XP_636472.1; XM_631380.1.
DR AlphaFoldDB; Q54IA7; -.
DR SMR; Q54IA7; -.
DR STRING; 44689.DDB0231414; -.
DR PaxDb; Q54IA7; -.
DR PRIDE; Q54IA7; -.
DR EnsemblProtists; EAL62970; EAL62970; DDB_G0288893.
DR GeneID; 8626855; -.
DR KEGG; ddi:DDB_G0288893; -.
DR dictyBase; DDB_G0288893; hemF.
DR eggNOG; KOG1518; Eukaryota.
DR HOGENOM; CLU_026169_0_1_1; -.
DR InParanoid; Q54IA7; -.
DR OMA; WFGGITD; -.
DR PhylomeDB; Q54IA7; -.
DR Reactome; R-DDI-189451; Heme biosynthesis.
DR UniPathway; UPA00251; UER00322.
DR PRO; PR:Q54IA7; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; IBA:GO_Central.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.1500.10; -; 1.
DR InterPro; IPR001260; Coprogen_oxidase_aer.
DR InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR PANTHER; PTHR10755; PTHR10755; 1.
DR Pfam; PF01218; Coprogen_oxidas; 1.
DR PIRSF; PIRSF000166; Coproporphyri_ox; 1.
DR PRINTS; PR00073; COPRGNOXDASE.
DR SUPFAM; SSF102886; SSF102886; 1.
PE 3: Inferred from homology;
KW Heme biosynthesis; Oxidoreductase; Porphyrin biosynthesis;
KW Reference proteome.
FT CHAIN 1..332
FT /note="Oxygen-dependent coproporphyrinogen-III oxidase"
FT /id="PRO_0000328892"
FT REGION 57..84
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT REGION 265..300
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT ACT_SITE 133
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 135..137
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 283..288
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 201
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
SQ SEQUENCE 332 AA; 38266 MW; E265FBDFDED984F2 CRC64;
MTSTNEPIPT EEEIKNGFKF IQDEICKFLI TTTGGHQQYS EDKWDYTKGS GGGISRVWEG
KEEEGFYLNQ DYQSQNNKCD KIEKGGVNFS YIVGSNLPSA AATQFKIAPD TKYIATGVSL
VIHPYNPNVP TIHMNVRYFE AGDVWWFGGG VDLTPVYPKL DQVVEFHSTL KKVCDQYGQE
ENKTSYALGK AECDSYFFLP HRGETRGVGG LFFDHLKSDK AKTWKFIYQL GLSFIDLYKP
FLVNNSSISY DKVQREYQLY RRSRYVEFNL LFDRGTKFGI LSEGRTESIL MSLPAVCKWK
YNYKPEENTP EANLLTFLRP RDWLNENQEN KN
