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HEM6_ECOLI
ID   HEM6_ECOLI              Reviewed;         299 AA.
AC   P36553;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase {ECO:0000255|HAMAP-Rule:MF_00333};
DE            Short=CPO {ECO:0000255|HAMAP-Rule:MF_00333};
DE            Short=Coprogen oxidase {ECO:0000255|HAMAP-Rule:MF_00333};
DE            Short=Coproporphyrinogenase {ECO:0000255|HAMAP-Rule:MF_00333};
DE            EC=1.3.3.3 {ECO:0000255|HAMAP-Rule:MF_00333};
GN   Name=hemF {ECO:0000255|HAMAP-Rule:MF_00333};
GN   OrderedLocusNames=b2436, JW2429;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8300522; DOI=10.1128/jb.176.3.673-680.1994;
RA   Troup B., Jahn M., Hungerer C., Jahn D.;
RT   "Isolation of the hemF operon containing the gene for the Escherichia coli
RT   aerobic coproporphyrinogen III oxidase by in vivo complementation of a
RT   yeast HEM13 mutant.";
RL   J. Bacteriol. 176:673-680(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-6, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, MASS SPECTROMETRY, ACTIVITY REGULATION, SUBUNIT, AND
RP   CRYSTALLIZATION.
RX   PubMed=13129604; DOI=10.1016/s0378-1097(03)00531-7;
RA   Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D.;
RT   "Oxygen-dependent coproporphyrinogen-III oxidase from Escherichia coli:
RT   one-step purification and biochemical characterisation.";
RL   FEMS Microbiol. Lett. 226:31-37(2003).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-18, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, COFACTOR, MUTAGENESIS OF HIS-96; HIS-106; HIS-145 AND HIS-175,
RP   ACTIVITY REGULATION, MASS SPECTROMETRY, REACTION MECHANISM, AND SUBUNIT.
RX   PubMed=12975365; DOI=10.1074/jbc.m308553200;
RA   Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D.;
RT   "Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia
RT   coli is stimulated by manganese.";
RL   J. Biol. Chem. 278:46625-46631(2003).
CC   -!- FUNCTION: Involved in the heme biosynthesis. Catalyzes the aerobic
CC       oxidative decarboxylation of propionate groups of rings A and B of
CC       coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-
CC       IX. {ECO:0000255|HAMAP-Rule:MF_00333, ECO:0000269|PubMed:12975365,
CC       ECO:0000269|PubMed:13129604}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC         protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00333,
CC         ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00333,
CC         ECO:0000269|PubMed:12975365};
CC   -!- ACTIVITY REGULATION: Inhibited by protoporphyrinogen-IX, by metal
CC       chelator (EGTA) and by low concentrations of heavy metal ions.
CC       {ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=2.6 uM for coproporphyrinogen-III (at pH 6 at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604};
CC         KM=3.1 uM for coproporphyrinogen-III (at pH 7)
CC         {ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604};
CC         Vmax=1.3 umol/min/mg enzyme (at pH 6 at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604};
CC         Note=kcat is 0.17 min(-1) for decarboxylation of coproporphyrinogen-
CC         III (at pH 6 and at 37 degrees Celsius).
CC         {ECO:0000269|PubMed:12975365};
CC       pH dependence:
CC         Optimum pH is between 6 and 7. {ECO:0000269|PubMed:12975365,
CC         ECO:0000269|PubMed:13129604};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC       route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00333}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00333,
CC       ECO:0000269|PubMed:12975365, ECO:0000269|PubMed:13129604}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MASS SPECTROMETRY: Mass=35390; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:13129604};
CC   -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC       family. {ECO:0000255|HAMAP-Rule:MF_00333}.
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DR   EMBL; X75413; CAA53167.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75489.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16319.1; -; Genomic_DNA.
DR   PIR; B36964; B36964.
DR   RefSeq; NP_416931.1; NC_000913.3.
DR   RefSeq; WP_000801365.1; NZ_LN832404.1.
DR   AlphaFoldDB; P36553; -.
DR   SMR; P36553; -.
DR   BioGRID; 4260574; 13.
DR   IntAct; P36553; 3.
DR   STRING; 511145.b2436; -.
DR   PaxDb; P36553; -.
DR   PRIDE; P36553; -.
DR   DNASU; 946908; -.
DR   EnsemblBacteria; AAC75489; AAC75489; b2436.
DR   EnsemblBacteria; BAA16319; BAA16319; BAA16319.
DR   GeneID; 946908; -.
DR   KEGG; ecj:JW2429; -.
DR   KEGG; eco:b2436; -.
DR   PATRIC; fig|1411691.4.peg.4295; -.
DR   EchoBASE; EB2106; -.
DR   eggNOG; COG0408; Bacteria.
DR   HOGENOM; CLU_026169_0_1_6; -.
DR   InParanoid; P36553; -.
DR   OMA; HDKGTLF; -.
DR   PhylomeDB; P36553; -.
DR   BioCyc; EcoCyc:COPROGENOXI-MON; -.
DR   BioCyc; MetaCyc:COPROGENOXI-MON; -.
DR   BRENDA; 1.3.3.3; 2026.
DR   UniPathway; UPA00251; UER00322.
DR   PRO; PR:P36553; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IDA:EcoliWiki.
DR   GO; GO:0004109; F:coproporphyrinogen oxidase activity; IDA:EcoCyc.
DR   GO; GO:0030145; F:manganese ion binding; IDA:EcoCyc.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0046906; F:tetrapyrrole binding; IMP:EcoliWiki.
DR   GO; GO:0006783; P:heme biosynthetic process; IMP:EcoliWiki.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IBA:GO_Central.
DR   GO; GO:0033194; P:response to hydroperoxide; IMP:EcoCyc.
DR   Gene3D; 3.40.1500.10; -; 1.
DR   HAMAP; MF_00333; Coprogen_oxidas; 1.
DR   InterPro; IPR001260; Coprogen_oxidase_aer.
DR   InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR   InterPro; IPR018375; Coprogen_oxidase_CS.
DR   PANTHER; PTHR10755; PTHR10755; 1.
DR   Pfam; PF01218; Coprogen_oxidas; 1.
DR   PIRSF; PIRSF000166; Coproporphyri_ox; 1.
DR   PRINTS; PR00073; COPRGNOXDASE.
DR   SUPFAM; SSF102886; SSF102886; 1.
DR   PROSITE; PS01021; COPROGEN_OXIDASE; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Heme biosynthesis; Manganese;
KW   Metal-binding; Oxidoreductase; Porphyrin biosynthesis; Reference proteome.
FT   CHAIN           1..299
FT                   /note="Oxygen-dependent coproporphyrinogen-III oxidase"
FT                   /id="PRO_0000109894"
FT   REGION          240..275
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   ACT_SITE        106
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   BINDING         92
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   BINDING         96
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000305"
FT   BINDING         106
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000305"
FT   BINDING         108..110
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   BINDING         145
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000305"
FT   BINDING         175
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000305"
FT   BINDING         258..260
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   SITE            175
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00333"
FT   MUTAGEN         96
FT                   /note="H->L: Complete loss of oxidase activity and absence
FT                   of manganese ions."
FT                   /evidence="ECO:0000269|PubMed:12975365"
FT   MUTAGEN         106
FT                   /note="H->L: Complete loss of oxidase activity and absence
FT                   of manganese ions."
FT                   /evidence="ECO:0000269|PubMed:12975365"
FT   MUTAGEN         145
FT                   /note="H->L: Complete loss of oxidase activity and absence
FT                   of manganese ions."
FT                   /evidence="ECO:0000269|PubMed:12975365"
FT   MUTAGEN         175
FT                   /note="H->L: Complete loss of oxidase activity and absence
FT                   of manganese ions."
FT                   /evidence="ECO:0000269|PubMed:12975365"
FT   MUTAGEN         274
FT                   /note="W->L: Complete loss of oxidase activity."
SQ   SEQUENCE   299 AA;  34323 MW;  53667E5C7B6D0198 CRC64;
     MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR NGGVFEQAGV
     NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAD
     PVWWFGGGFD LTPFYGFEED AIHWHRTARD LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR
     GIGGLFFDDL NTPDFDRCFA FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY
     VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV
 
 
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