HEM6_ORYSJ
ID HEM6_ORYSJ Reviewed; 399 AA.
AC Q7XPL2; Q0JA90; Q84VE2;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase, chloroplastic;
DE Short=Coprogen oxidase;
DE Short=Coproporphyrinogenase;
DE EC=1.3.3.3;
DE Flags: Precursor;
GN Name=CPX; OrderedLocusNames=Os04g0610800, LOC_Os04g52130;
GN ORFNames=OSJNBa0085I10.14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 79-399.
RC STRAIN=cv. Nipponbare;
RX PubMed=14750518; DOI=10.1023/b:plan.0000007001.30865.0f;
RA Cooper B., Hutchison D., Park S., Guimil S., Luginbuehl P., Ellero C.,
RA Goff S.A., Glazebrook J.;
RT "Identification of rice (Oryza sativa) proteins linked to the cyclin-
RT mediated regulation of the cell cycle.";
RL Plant Mol. Biol. 53:273-279(2003).
CC -!- FUNCTION: Involved in the heme and chlorophyll biosynthesis. Catalyzes
CC the aerobic oxidative decarboxylation of propionate groups of rings A
CC and B of coproporphyrinogen-III to yield the vinyl groups in
CC protoporphyrinogen-IX (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC route): step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15747.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL606684; CAE03569.2; -; Genomic_DNA.
DR EMBL; AP008210; BAF15747.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY224543; AAO72663.1; -; mRNA.
DR RefSeq; XP_015633531.1; XM_015778045.1.
DR AlphaFoldDB; Q7XPL2; -.
DR SMR; Q7XPL2; -.
DR BioGRID; 805988; 1.
DR STRING; 4530.OS04T0610800-01; -.
DR PaxDb; Q7XPL2; -.
DR PRIDE; Q7XPL2; -.
DR GeneID; 4336951; -.
DR KEGG; osa:4336951; -.
DR eggNOG; KOG1518; Eukaryota.
DR HOGENOM; CLU_026169_0_0_1; -.
DR InParanoid; Q7XPL2; -.
DR OrthoDB; 1080991at2759; -.
DR UniPathway; UPA00251; UER00322.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q7XPL2; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.1500.10; -; 1.
DR InterPro; IPR001260; Coprogen_oxidase_aer.
DR InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR InterPro; IPR018375; Coprogen_oxidase_CS.
DR PANTHER; PTHR10755; PTHR10755; 1.
DR Pfam; PF01218; Coprogen_oxidas; 1.
DR PIRSF; PIRSF000166; Coproporphyri_ox; 1.
DR PRINTS; PR00073; COPRGNOXDASE.
DR SUPFAM; SSF102886; SSF102886; 1.
DR PROSITE; PS01021; COPROGEN_OXIDASE; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll biosynthesis; Chloroplast; Heme biosynthesis; Oxidoreductase;
KW Plastid; Porphyrin biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..399
FT /note="Oxygen-dependent coproporphyrinogen-III oxidase,
FT chloroplastic"
FT /id="PRO_0000376070"
FT REGION 20..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 55..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 129..138
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT REGION 337..372
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT ACT_SITE 199
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 185
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 201..203
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 355..360
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 272
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT CONFLICT 374
FT /note="D -> Y (in Ref. 5; AAO72663)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 43871 MW; C41A89FBCF860079 CRC64;
MAPSLLTTPS QALALAPGAA ASRVGGGGGG SARVSFPSGR VQRRGALGMR VRASVAIEKE
TPESEPPPTF LREDGSGAGS GSVRERFEAM IRRVQGEVCA ALEEADGSGA RFVEDVWSRP
GGGGGISRVL QDGRVFEKAG VNVSVVYGVM PPDAYRAAKG EAGKNGAAAD GPKAGPVPFF
AAGISSVLHP KNPFAPTLHF NYRYFETDAP KDAPGAPRQW WFGGGTDLTP SYIIEEDVKH
FHSVQKQACD KFDPSFYPRF KKWCDDYFYI KHRNERRGLG GIFFDDLNDY DQEMLLNFAT
ECADSVVPAY IPIIERRKDT PFTEEHKAWQ QLRRGRYVEF NLVYDRGTTF GLKTGGRIES
ILVSLPLTAR WQYDHTPEEG TEERKLLDAC INPKEWLDL
