HEM6_SCHPO
ID HEM6_SCHPO Reviewed; 312 AA.
AC Q9UTE2;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable oxygen-dependent coproporphyrinogen-III oxidase;
DE Short=COX;
DE Short=Coprogen oxidase;
DE Short=Coproporphyrinogenase;
DE EC=1.3.3.3;
GN Name=hem13; ORFNames=SPAC222.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Involved in the heme biosynthesis. Catalyzes the aerobic
CC oxidative decarboxylation of propionate groups of rings A and B of
CC coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-
CC IX (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC route): step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC family. {ECO:0000305}.
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DR EMBL; CU329670; CAB60703.1; -; Genomic_DNA.
DR PIR; T50152; T50152.
DR RefSeq; NP_593150.1; NM_001018547.2.
DR AlphaFoldDB; Q9UTE2; -.
DR SMR; Q9UTE2; -.
DR BioGRID; 278422; 4.
DR IntAct; Q9UTE2; 1.
DR STRING; 4896.SPAC222.11.1; -.
DR iPTMnet; Q9UTE2; -.
DR MaxQB; Q9UTE2; -.
DR PaxDb; Q9UTE2; -.
DR EnsemblFungi; SPAC222.11.1; SPAC222.11.1:pep; SPAC222.11.
DR GeneID; 2541934; -.
DR KEGG; spo:SPAC222.11; -.
DR PomBase; SPAC222.11; hem13.
DR VEuPathDB; FungiDB:SPAC222.11; -.
DR eggNOG; KOG1518; Eukaryota.
DR HOGENOM; CLU_026169_0_0_1; -.
DR InParanoid; Q9UTE2; -.
DR OMA; HDKGTLF; -.
DR PhylomeDB; Q9UTE2; -.
DR Reactome; R-SPO-189451; Heme biosynthesis.
DR UniPathway; UPA00251; UER00322.
DR PRO; PR:Q9UTE2; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; ISO:PomBase.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IMP:PomBase.
DR Gene3D; 3.40.1500.10; -; 1.
DR InterPro; IPR001260; Coprogen_oxidase_aer.
DR InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR InterPro; IPR018375; Coprogen_oxidase_CS.
DR PANTHER; PTHR10755; PTHR10755; 1.
DR Pfam; PF01218; Coprogen_oxidas; 1.
DR PIRSF; PIRSF000166; Coproporphyri_ox; 1.
DR PRINTS; PR00073; COPRGNOXDASE.
DR SUPFAM; SSF102886; SSF102886; 1.
DR PROSITE; PS01021; COPROGEN_OXIDASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Heme biosynthesis; Oxidoreductase; Porphyrin biosynthesis;
KW Reference proteome.
FT CHAIN 1..312
FT /note="Probable oxygen-dependent coproporphyrinogen-III
FT oxidase"
FT /id="PRO_0000109877"
FT REGION 53..62
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT REGION 252..288
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
FT ACT_SITE 118
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 120..122
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 271..276
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 187
FT /note="Important for dimerization"
FT /evidence="ECO:0000250"
SQ SEQUENCE 312 AA; 36244 MW; 33C785A534977583 CRC64;
MSDITVEPIG KQMEKLILDV QQEIVAGLEA VDGQKFFQDK WTKGEGGYGI SCVIQDGNVF
EKGGVNTSIV QGKLNQDAVQ RMRANHEGID RTAKELPFFA AGISMVIHPR NPMAPTTHLN
YRYFELVNSD GKKIWWFGGG ADLTPSILFE EDGKHFHKLH KEACDRHDPT FYPRFKKWAD
EYFLIKHRKE TRGIGGIFFD DLSEKDPQEL FAFVKDCAHT FLPAYVPIME KRKNMEFTED
DKEFQLIRRG YYAEFNVMYD RGTWFGLQAP EPRVESILMT LPLHASWRYK YEPKQERHKA
LLKVTHTPIE WC
