ID   HEM6_SOYBN              Reviewed;         385 AA.
AC   P35055;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Oxygen-dependent coproporphyrinogen-III oxidase, chloroplastic;
DE            Short=Coprogen oxidase;
DE            Short=Coproporphyrinogenase;
DE            EC=1.3.3.3;
DE   Flags: Precursor;
GN   Name=CPX;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Evans; TISSUE=Root nodule;
RX   PubMed=8219054; DOI=10.1007/bf00021417;
RA   Madsen O., Sandal L., Sandal N.N., Marcker K.A.;
RT   "A soybean coproporphyrinogen oxidase gene is highly expressed in root
RT   nodules.";
RL   Plant Mol. Biol. 23:35-43(1993).
CC   -!- FUNCTION: Involved in the heme and chlorophyll biosynthesis. Catalyzes
CC       the aerobic oxidative decarboxylation of propionate groups of rings A
CC       and B of coproporphyrinogen-III to yield the vinyl groups in
CC       protoporphyrinogen-IX (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O +
CC         protoporphyrinogen IX; Xref=Rhea:RHEA:18257, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57307, ChEBI:CHEBI:57309; EC=1.3.3.3;
CC   -!- ACTIVITY REGULATION: Probably regulated by oxygen tension.
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2
CC       route): step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in nodules and to a lesser extent
CC       in leaves. Not detected in roots.
CC   -!- DEVELOPMENTAL STAGE: Expression starts 10-12 days after infection and
CC       continues during the lifetime of the nodule.
CC   -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen-III oxidase
CC       family. {ECO:0000305}.
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DR   EMBL; X71083; CAA50401.1; -; Genomic_DNA.
DR   EMBL; X71083; CAA50400.1; -; Genomic_DNA.
DR   PIR; S39523; S39523.
DR   AlphaFoldDB; P35055; -.
DR   SMR; P35055; -.
DR   STRING; 3847.GLYMA14G00620.2; -.
DR   PRIDE; P35055; -.
DR   ProMEX; P35055; -.
DR   eggNOG; KOG1518; Eukaryota.
DR   InParanoid; P35055; -.
DR   BioCyc; MetaCyc:MON-11768; -.
DR   UniPathway; UPA00251; UER00322.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004109; F:coproporphyrinogen oxidase activity; IBA:GO_Central.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.40.1500.10; -; 1.
DR   InterPro; IPR001260; Coprogen_oxidase_aer.
DR   InterPro; IPR036406; Coprogen_oxidase_aer_sf.
DR   InterPro; IPR018375; Coprogen_oxidase_CS.
DR   PANTHER; PTHR10755; PTHR10755; 1.
DR   Pfam; PF01218; Coprogen_oxidas; 1.
DR   PIRSF; PIRSF000166; Coproporphyri_ox; 1.
DR   PRINTS; PR00073; COPRGNOXDASE.
DR   SUPFAM; SSF102886; SSF102886; 1.
DR   PROSITE; PS01021; COPROGEN_OXIDASE; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; Heme biosynthesis; Oxidoreductase;
KW   Plastid; Porphyrin biosynthesis; Reference proteome; Transit peptide.
FT   TRANSIT         1..67
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           68..385
FT                   /note="Oxygen-dependent coproporphyrinogen-III oxidase,
FT                   chloroplastic"
FT                   /id="PRO_0000006033"
FT   REGION          124..133
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   REGION          325..360
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        187
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         173
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         189..191
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         343..348
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            260
FT                   /note="Important for dimerization"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   385 AA;  43265 MW;  C3FE1163CF80A09B CRC64;
     MMHCASIVSA PSYAFPFRSG SASTTPTAIS LTKRSWKPPP SMAKGPVRAT VSIEKETPEA
     NRPETFLRGV DEAQSSTSVR ARFEKMIREA QDTVCSALEA ADGGAQFKED VWSRPGGGGG
     ISRVLQDGAV WEKAGVNVSV VYGVMPPDAY RAAKGVPTDQ KPGPVPFFAA GISSVLHPKN
     PFAPTLHFNY RYFETDAPKD APGAPRQWWF GGGTDLTPAY IFEEDVKHFH SIQKQACDKF
     EPTFYPRFKK WCDDYFYIKH RGERRGLGGI FFDDLNDYDQ EMLLSFATEC ANSVIPAYLP
     IIEKRKDLPF NDHQKAWQQL RRGRYVEFNL VYDRGTTFGL KTGGRIESIL VSLPLTARWE
     YDHKPEEGSE EWKLLDACIN PKEWI