ID   HEMAT_BACSU             Reviewed;         432 AA.
AC   O07621;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Heme-based aerotactic transducer HemAT;
GN   Name=hemAT; Synonyms=yhfV; OrderedLocusNames=BSU10380;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA   Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA   Venema G., Bron S.;
RT   "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT   subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT   many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL   Microbiology 144:859-875(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=10676961; DOI=10.1038/35000570;
RA   Hou S., Larsen R.W., Boudko D., Riley C.W., Karatan E., Zimmer M.,
RA   Ordal G.W., Alam M.;
RT   "Myoglobin-like aerotaxis transducers in Archaea and Bacteria.";
RL   Nature 403:540-544(2000).
RN   [4]
RP   RESONANCE RAMAN SPECTROSCOPY, AND SUBUNIT.
RX   PubMed=11821422; DOI=10.1074/jbc.m112256200;
RA   Aono S., Kato T., Matsuki M., Nakajima H., Ohta T., Uchida T., Kitagawa T.;
RT   "Resonance Raman and ligand binding studies of the oxygen-sensing signal
RT   transducer protein HemAT from Bacillus subtilis.";
RL   J. Biol. Chem. 277:13528-13538(2002).
CC   -!- FUNCTION: Heme-containing signal transducer responsible for aerotaxis,
CC       the migratory response toward or away from oxygen.
CC       {ECO:0000269|PubMed:10676961}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000305|PubMed:11821422}.
CC   -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC       family. {ECO:0000305}.
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DR   EMBL; Y14084; CAA74545.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12878.1; -; Genomic_DNA.
DR   PIR; C69832; C69832.
DR   RefSeq; NP_388919.1; NC_000964.3.
DR   RefSeq; WP_003245216.1; NZ_JNCM01000035.1.
DR   PDB; 1OR4; X-ray; 2.15 A; A/B=1-178.
DR   PDB; 1OR6; X-ray; 2.71 A; A/B=1-178.
DR   PDBsum; 1OR4; -.
DR   PDBsum; 1OR6; -.
DR   AlphaFoldDB; O07621; -.
DR   SMR; O07621; -.
DR   IntAct; O07621; 3.
DR   STRING; 224308.BSU10380; -.
DR   jPOST; O07621; -.
DR   PaxDb; O07621; -.
DR   PRIDE; O07621; -.
DR   EnsemblBacteria; CAB12878; CAB12878; BSU_10380.
DR   GeneID; 936320; -.
DR   KEGG; bsu:BSU10380; -.
DR   PATRIC; fig|224308.179.peg.1116; -.
DR   eggNOG; COG0840; Bacteria.
DR   InParanoid; O07621; -.
DR   OMA; YVGQYGV; -.
DR   PhylomeDB; O07621; -.
DR   BioCyc; BSUB:BSU10380-MON; -.
DR   EvolutionaryTrace; O07621; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR   GO; GO:0009454; P:aerotaxis; IDA:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IDA:UniProtKB.
DR   CDD; cd01068; globin_sensor; 1.
DR   Gene3D; 1.10.490.10; -; 1.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR044398; Globin-sensor_dom.
DR   InterPro; IPR012292; Globin/Proto.
DR   InterPro; IPR004089; MCPsignal_dom.
DR   InterPro; IPR039379; Protoglobin_sensor_dom.
DR   Pfam; PF00015; MCPsignal; 1.
DR   Pfam; PF11563; Protoglobin; 1.
DR   SMART; SM00283; MA; 1.
DR   SUPFAM; SSF46458; SSF46458; 1.
DR   PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Heme; Iron; Metal-binding; Reference proteome; Transducer.
FT   CHAIN           1..432
FT                   /note="Heme-based aerotactic transducer HemAT"
FT                   /id="PRO_0000110567"
FT   DOMAIN          184..420
FT                   /note="Methyl-accepting transducer"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
FT   HELIX           18..21
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           29..31
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           32..40
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           45..73
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           77..86
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           89..102
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           110..125
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           130..151
FT                   /evidence="ECO:0007829|PDB:1OR4"
FT   HELIX           155..176
FT                   /evidence="ECO:0007829|PDB:1OR4"
SQ   SEQUENCE   432 AA;  48767 MW;  87D0655974070B64 CRC64;
     MLFKKDRKQE TAYFSDSNGQ QKNRIQLTNK HADVKKQLKM VRLGDAELYV LEQLQPLIQE
     NIVNIVDAFY KNLDHESSLM DIINDHSSVD RLKQTLKRHI QEMFAGVIDD EFIEKRNRIA
     SIHLRIGLLP KWYMGAFQEL LLSMIDIYEA SITNQQELLK AIKATTKILN LEQQLVLEAF
     QSEYNQTRDE QEEKKNLLHQ KIQETSGSIA NLFSETSRSV QELVDKSEGI SQASKAGTVT
     SSTVEEKSIG GKKELEVQQK QMNKIDTSLV QIEKEMVKLD EIAQQIEKIF GIVTGIAEQT
     NLLSLNASIE SARAGEHGKG FAVVANEVRK LSEDTKKTVS TVSELVNNTN TQINIVSKHI
     KDVNELVSES KEKMTQINRL FDEIVHSMKI SKEQSGKIDV DLQAFLGGLQ EVSRAVSHVA
     ASVDSLVILT EE