ANL2_ARATH
ID ANL2_ARATH Reviewed; 802 AA.
AC Q0WV12; O65281; P93041; Q9SWZ6;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Homeobox-leucine zipper protein ANTHOCYANINLESS 2 {ECO:0000303|PubMed:10402424};
DE AltName: Full=HD-ZIP protein ANL2 {ECO:0000303|PubMed:10402424};
DE AltName: Full=Homeodomain protein AHDP {ECO:0000303|PubMed:10402424};
DE AltName: Full=Homeodomain transcription factor ANL2 {ECO:0000303|PubMed:10402424};
GN Name=ANL2 {ECO:0000303|PubMed:10402424};
GN OrderedLocusNames=At4g00730 {ECO:0000312|Araport:AT4G00730};
GN ORFNames=F6N23.10 {ECO:0000312|EMBL:AAC13617.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8989876; DOI=10.2307/3870458;
RA Lu P., Porat R., Nadeau J.A., O'Neill S.D.;
RT "Identification of a meristem L1 layer-specific gene in Arabidopsis that is
RT expressed during embryonic pattern formation and defines a new class of
RT homeobox genes.";
RL Plant Cell 8:2155-2168(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10402424; DOI=10.2307/3870744;
RA Kubo H., Peeters A.J.M., Aarts M.G.M., Pereira A., Koornneef M.;
RT "ANTHOCYANINLESS2, a homeobox gene affecting anthocyanin distribution and
RT root development in Arabidopsis.";
RL Plant Cell 11:1217-1226(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=10809443; DOI=10.1023/a:1006368316413;
RA Tavares R., Aubourg S., Lecharny A., Kreis M.;
RT "Organization and structural evolution of four multigene families in
RT Arabidopsis thaliana: AtLCAD, AtLGT, AtMYST and AtHD-GL2.";
RL Plant Mol. Biol. 42:703-717(2000).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16778018; DOI=10.1104/pp.106.077388;
RA Nakamura M., Katsumata H., Abe M., Yabe N., Komeda Y., Yamamoto K.T.,
RA Takahashi T.;
RT "Characterization of the class IV homeodomain-leucine zipper gene family in
RT Arabidopsis.";
RL Plant Physiol. 141:1363-1375(2006).
RN [8]
RP INTERACTION WITH AIL7/PLT7; ANT; BBM AND AIL1.
RC STRAIN=cv. Columbia;
RX PubMed=25564655; DOI=10.1242/dev.117168;
RA Horstman A., Fukuoka H., Muino J.M., Nitsch L., Guo C., Passarinho P.,
RA Sanchez-Perez G., Immink R., Angenent G., Boutilier K.;
RT "AIL and HDG proteins act antagonistically to control cell proliferation.";
RL Development 142:454-464(2015).
CC -!- FUNCTION: Probable transcription factor involved in the regulation of
CC the tissue-specific accumulation of anthocyanins and in cellular
CC organization of the primary root. {ECO:0000269|PubMed:10402424}.
CC -!- SUBUNIT: Interacts with AIL7/PLT7, ANT, BBM and AIL1.
CC {ECO:0000269|PubMed:25564655}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q0WV12-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves and floral buds.
CC {ECO:0000269|PubMed:10402424}.
CC -!- DISRUPTION PHENOTYPE: Plants display a strong reduction of anthocyanin
CC content on the adaxial side of rosette leaves, a slight reduction onf
CC the abaxial side, and extra cells between cortical and epidermal layers
CC in roots. {ECO:0000269|PubMed:10402424}.
CC -!- SIMILARITY: Belongs to the HD-ZIP homeobox family. Class IV subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB41901.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAB41901.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAC13617.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80882.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U85254; AAB41901.1; ALT_SEQ; mRNA.
DR EMBL; AF077335; AAD47139.1; -; Genomic_DNA.
DR EMBL; AF058919; AAC13617.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161472; CAB80882.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE81926.1; -; Genomic_DNA.
DR EMBL; AK226968; BAE99036.1; -; mRNA.
DR PIR; T01237; T01237.
DR RefSeq; NP_567183.2; NM_116298.4. [Q0WV12-1]
DR AlphaFoldDB; Q0WV12; -.
DR SMR; Q0WV12; -.
DR STRING; 3702.AT4G00730.1; -.
DR iPTMnet; Q0WV12; -.
DR PaxDb; Q0WV12; -.
DR PRIDE; Q0WV12; -.
DR ProteomicsDB; 244989; -. [Q0WV12-1]
DR EnsemblPlants; AT4G00730.1; AT4G00730.1; AT4G00730. [Q0WV12-1]
DR GeneID; 828022; -.
DR Gramene; AT4G00730.1; AT4G00730.1; AT4G00730. [Q0WV12-1]
DR KEGG; ath:AT4G00730; -.
DR Araport; AT4G00730; -.
DR TAIR; locus:2127008; AT4G00730.
DR eggNOG; ENOG502QUAY; Eukaryota.
DR HOGENOM; CLU_015002_2_1_1; -.
DR InParanoid; Q0WV12; -.
DR OMA; PCIGMRP; -.
DR OrthoDB; 226429at2759; -.
DR PhylomeDB; Q0WV12; -.
DR PRO; PR:Q0WV12; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WV12; baseline and differential.
DR Genevisible; Q0WV12; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008289; F:lipid binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0043481; P:anthocyanin accumulation in tissues in response to UV light; IMP:TAIR.
DR GO; GO:0006723; P:cuticle hydrocarbon biosynthetic process; IMP:TAIR.
DR GO; GO:0009827; P:plant-type cell wall modification; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IMP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR042160; GLABRA2/ANL2/PDF2/ATML1-like.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR002913; START_lipid-bd_dom.
DR PANTHER; PTHR45654; PTHR45654; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF01852; START; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00234; START; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50848; START; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..802
FT /note="Homeobox-leucine zipper protein ANTHOCYANINLESS 2"
FT /id="PRO_0000331657"
FT DOMAIN 315..546
FT /note="START"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00197"
FT DNA_BIND 134..193
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 71..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 182..221
FT /evidence="ECO:0000255"
FT COMPBIAS 78..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..115
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 286
FT /note="Missing (in Ref. 2; AAD47139)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="A -> P (in Ref. 2; AAD47139)"
FT /evidence="ECO:0000305"
FT CONFLICT 548
FT /note="I -> M (in Ref. 2; AAD47139)"
FT /evidence="ECO:0000305"
FT CONFLICT 735..736
FT /note="LP -> SS (in Ref. 2; AAD47139)"
FT /evidence="ECO:0000305"
FT CONFLICT 801
FT /note="E -> G (in Ref. 2; AAD47139)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 802 AA; 87194 MW; C7BADF1697E708A8 CRC64;
MNFGSLFDNT PGGGSTGARL LSGLSYGNHT AATNVLPGGA MAQAAAAASL FSPPLTKSVY
ASSGLSLALE QPERGTNRGE ASMRNNNNVG GGGDTFDGSV NRRSREEEHE SRSGSDNVEG
ISGEDQDAAD KPPRKKRYHR HTPQQIQELE SMFKECPHPD EKQRLELSKR LCLETRQVKF
WFQNRRTQMK TQLERHENAL LRQENDKLRA ENMSIREAMR NPICTNCGGP AMLGDVSLEE
HHLRIENARL KDELDRVCNL TGKFLGHHHN HHYNSSLELA VGTNNNGGHF AFPPDFGGGG
GCLPPQQQQS TVINGIDQKS VLLELALTAM DELVKLAQSE EPLWVKSLDG ERDELNQDEY
MRTFSSTKPT GLATEASRTS GMVIINSLAL VETLMDSNRW TEMFPCNVAR ATTTDVISGG
MAGTINGALQ LMNAELQVLS PLVPVRNVNF LRFCKQHAEG VWAVVDVSID PVRENSGGAP
VIRRLPSGCV VQDVSNGYSK VTWVEHAEYD ENQIHQLYRP LLRSGLGFGS QRWLATLQRQ
CECLAILISS SVTSHDNTSI TPGGRKSMLK LAQRMTFNFC SGISAPSVHN WSKLTVGNVD
PDVRVMTRKS VDDPGEPPGI VLSAATSVWL PAAPQRLYDF LRNERMRCEW DILSNGGPMQ
EMAHITKGQD QGVSLLRSNA MNANQSSMLI LQETCIDASG ALVVYAPVDI PAMHVVMNGG
DSSYVALLPS GFAVLPDGGI DGGGSGDGDQ RPVGGGSLLT VAFQILVNNL PTAKLTVESV
ETVNNLISCT VQKIRAALQC ES
