HEMGN_MOUSE
ID HEMGN_MOUSE Reviewed; 503 AA.
AC Q9ERZ0; B1AVH8; Q3TAE6; Q80YT2; Q8CF29;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Hemogen;
DE AltName: Full=Hemopoietic gene protein;
DE AltName: Full=Negative differentiation regulator protein;
DE Short=mNDR;
GN Name=Hemgn; Synonyms=Ndr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Fetal heart;
RX PubMed=11404085; DOI=10.1016/s0925-4773(01)00376-8;
RA Yang L.V., Nicholson R.H., Kaplan J., Galy A., Li L.;
RT "Hemogen is a novel nuclear factor specifically expressed in mouse
RT hematopoietic development and its human homologue EDAG maps to chromosome
RT 9q22, a region containing breakpoints of hematological neoplasms.";
RL Mech. Dev. 104:105-111(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Liu C.-C., Ch'ang L.-Y.;
RT "Effects of mNDR in spermatogenesis.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Embryo, Spleen, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=14648837; DOI=10.1002/dvdy.10399;
RA Yang L.V., Heng H.H., Wan J., Southwood C.M., Gow A., Li L.;
RT "Alternative promoters and polyadenylation regulate tissue-specific
RT expression of Hemogen isoforms during hematopoiesis and spermatogenesis.";
RL Dev. Dyn. 228:606-616(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-153; SER-158;
RP SER-171; SER-213; SER-223; SER-241; SER-269; THR-286 AND SER-387, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulates the proliferation and differentiation of
CC hematopoietic cells. Overexpression block the TPA-induced
CC megakaryocytic differentiation in the K562 cell model. May also prevent
CC cell apoptosis through the activation of the nuclear factor-kappa B
CC (NF-kB) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11404085}.
CC -!- TISSUE SPECIFICITY: Expressed in hematopoietic precursor cells. Highly
CC expressed in bone marrow, the red pulp of the spleen and round
CC spermatids. Weakly expressed in peripheral blood cells.
CC {ECO:0000269|PubMed:11404085, ECO:0000269|PubMed:14648837}.
CC -!- DEVELOPMENTAL STAGE: Expressed at 8.5 dpc in the blood islands of the
CC yolk sac and in the circulating primitive blood cells. Expressed in the
CC circulating blood cells at 9.5 dpc and 10.5 dpc. Detected in the
CC developing hepatic primordia at 10.5 dpc. From 11.5 dpc to 14.5 dpc,
CC exclusively expressed in the fetal liver. {ECO:0000269|PubMed:11404085,
CC ECO:0000269|PubMed:14648837}.
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DR EMBL; AF269248; AAG33367.1; -; mRNA.
DR EMBL; AY699986; AAT94243.1; -; mRNA.
DR EMBL; AK006588; BAC25148.2; -; mRNA.
DR EMBL; AK077692; BAC36959.1; -; mRNA.
DR EMBL; AK156536; BAE33747.1; -; mRNA.
DR EMBL; AK171890; BAE42722.1; -; mRNA.
DR EMBL; AL683884; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC050798; AAH50798.1; -; mRNA.
DR CCDS; CCDS18150.1; -.
DR RefSeq; NP_444379.1; NM_053149.2.
DR AlphaFoldDB; Q9ERZ0; -.
DR BioGRID; 220414; 3.
DR STRING; 10090.ENSMUSP00000103393; -.
DR iPTMnet; Q9ERZ0; -.
DR PhosphoSitePlus; Q9ERZ0; -.
DR EPD; Q9ERZ0; -.
DR jPOST; Q9ERZ0; -.
DR MaxQB; Q9ERZ0; -.
DR PaxDb; Q9ERZ0; -.
DR PeptideAtlas; Q9ERZ0; -.
DR PRIDE; Q9ERZ0; -.
DR ProteomicsDB; 269824; -.
DR Antibodypedia; 14425; 206 antibodies from 24 providers.
DR DNASU; 93966; -.
DR Ensembl; ENSMUST00000071096; ENSMUSP00000066383; ENSMUSG00000028332.
DR Ensembl; ENSMUST00000107764; ENSMUSP00000103393; ENSMUSG00000028332.
DR GeneID; 93966; -.
DR KEGG; mmu:93966; -.
DR UCSC; uc008str.1; mouse.
DR CTD; 55363; -.
DR MGI; MGI:2136910; Hemgn.
DR VEuPathDB; HostDB:ENSMUSG00000028332; -.
DR eggNOG; ENOG502SBFR; Eukaryota.
DR GeneTree; ENSGT00390000004522; -.
DR HOGENOM; CLU_569802_0_0_1; -.
DR InParanoid; Q9ERZ0; -.
DR OMA; EIHQETP; -.
DR OrthoDB; 801077at2759; -.
DR PhylomeDB; Q9ERZ0; -.
DR TreeFam; TF338654; -.
DR BioGRID-ORCS; 93966; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9ERZ0; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9ERZ0; protein.
DR Bgee; ENSMUSG00000028332; Expressed in fetal liver hematopoietic progenitor cell and 84 other tissues.
DR Genevisible; Q9ERZ0; MM.
DR GO; GO:0005654; C:nucleoplasm; IDA:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045667; P:regulation of osteoblast differentiation; ISO:MGI.
DR InterPro; IPR033272; Hemogen.
DR PANTHER; PTHR15993; PTHR15993; 3.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..503
FT /note="Hemogen"
FT /id="PRO_0000245362"
FT REGION 1..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 7..87
FT /note="Necessary for nuclear localization"
FT /evidence="ECO:0000250"
FT REGION 381..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..437
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..503
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 90
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AZ54"
FT MOD_RES 103
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 124
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BXL5"
FT MOD_RES 153
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 158
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 171
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 213
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 228
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BXL5"
FT MOD_RES 241
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 269
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 286
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 387
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 394
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AZ54"
FT CONFLICT 472
FT /note="E -> G (in Ref. 5; AAH50798)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 503 AA; 55043 MW; B1027B81B55BB08D CRC64;
MDMGKGRPRL KLPQMPEAHP QKSCAPDIIG SWSLRNREQL RKRKAEAQGR QTSQWLLGEQ
KKRKYQRTGK GNKRGRKRQG NVEQKAEPWS QTERERVQEV LVSAEEETEH PGNSATEALP
LVPSPTKAVP ADQCSEAHQE SIQCQERAIQ NHSQTHLSPT TCQGIAVLQH SPKMCQDMAE
PEVFSPNMCQ ETAVPQTYPP KALEEMAAAE PLSPKMCQET TVSPNHSSKV PQDMAGPEAL
SPNMCQEPTV PQEHTLKMCH DVARPEVLSP KTHQEMAVPK AFPCVTPGDA AGLEGCAPKA
LPQSDVAEGC PLDTTPTSVT PEQTTSDPDL GMAVTEGFFS EARECTVSEG VSTKTHQEAV
EPEFISHETY KEFTVPIVSS QKTIQESPEP EQYSPETCQP IPGPENYSLE TCHEMSGPED
LSIKTCQDRE EPKHSLPEGA QKVGGAQGQD ADAQDSENAG AFSQDFTEME EENKADQDPE
APASPQGSQE TCPENGIYSS ALF
