ID   HEMH_ORYSI              Reviewed;         526 AA.
AC   A2Y3Q5; O22101; Q6L4M5;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Ferrochelatase-2, chloroplastic;
DE            EC=4.99.1.1;
DE   AltName: Full=Ferrochelatase II;
DE   AltName: Full=Heme synthase 2;
DE   AltName: Full=Protoheme ferro-lyase 2;
DE   Flags: Precursor;
GN   Name=HEMH; ORFNames=OsI_018948;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 33-526.
RA   Kanjo N., Inokuchi H.;
RT   "Nucleotide sequence of a cDNA clone encoding ferrochelatase from rice.";
RL   (er) Plant Gene Register PGR97-175(1997).
CC   -!- FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + heme b = Fe(2+) + protoporphyrin IX;
CC         Xref=Rhea:RHEA:22584, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033,
CC         ChEBI:CHEBI:57306, ChEBI:CHEBI:60344; EC=4.99.1.1;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoheme
CC       biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the ferrochelatase family. {ECO:0000305}.
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DR   EMBL; CM000130; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AB007120; BAA22284.1; -; mRNA.
DR   AlphaFoldDB; A2Y3Q5; -.
DR   SMR; A2Y3Q5; -.
DR   STRING; 39946.A2Y3Q5; -.
DR   UniPathway; UPA00252; UER00325.
DR   Proteomes; UP000007015; Chromosome 5.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004325; F:ferrochelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006783; P:heme biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00419; Ferrochelatase_C; 1.
DR   CDD; cd03411; Ferrochelatase_N; 1.
DR   HAMAP; MF_00323; Ferrochelatase; 1.
DR   InterPro; IPR001015; Ferrochelatase.
DR   InterPro; IPR019772; Ferrochelatase_AS.
DR   InterPro; IPR033644; Ferrochelatase_C.
DR   InterPro; IPR033659; Ferrochelatase_N.
DR   PANTHER; PTHR11108; PTHR11108; 1.
DR   Pfam; PF00762; Ferrochelatase; 1.
DR   TIGRFAMs; TIGR00109; hemH; 1.
DR   PROSITE; PS00534; FERROCHELATASE; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Heme biosynthesis; Iron; Lyase; Plastid;
KW   Porphyrin biosynthesis; Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..526
FT                   /note="Ferrochelatase-2, chloroplastic"
FT                   /id="PRO_0000295023"
FT   CONFLICT        70
FT                   /note="K -> R (in Ref. 2; BAA22284)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   526 AA;  58590 MW;  95137FEEDA8F3E5F CRC64;
     MWSSSQASTR GVIEVGRVEA GPSHFPKRPA PRNSSRVNLS RTYAIKSCSV SSRTGLCLGQ
     CYHKKSSACK CKLGWSSQPL SSLRHHLRVH SSASEAVLTS QSDFTKLLVG NEKIGVLLLN
     LGGPETLDDV QPFLFNLFAD PDIIRLPRLF RFLQKPLAQF ISVVRAPKSK EGYASIGGGS
     PLRQITDAQA EALRKALCDK DIPAKVYVGM RYWHPFTEEA IEQIKRDGIT KLVVLPLYPQ
     FSISTSGSSL RLLEGIFRED EYLVNMQHTV IPSWYQREGY IKAMATLIEK ELRTFSEPQK
     VMIFFSAHGV PLAYVEEAGD PYKAEMEECV DLIMEELEKR GITNSCTLAY QSRVGPVEWL
     RPYTDETIIE LGQKGVKSLL AVPISFVSEH IETLEEIDVE YKELALESGI KHWGRVPALG
     CEPTFITDLA DAVIESLPYV GAMAVSNLEA RQPLVPLGSV EELLAAYDSK RDELPPPVTV
     WEWGWTKSAE TWNGRAAMLA VLALLVLEVT TGEGFLHQWG ILPLFH