ID   HEMH_PSEAE              Reviewed;         340 AA.
AC   Q9HVD7;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2001, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Ferrochelatase {ECO:0000255|HAMAP-Rule:MF_00323};
DE            EC=4.99.1.1 {ECO:0000255|HAMAP-Rule:MF_00323};
DE   AltName: Full=Heme synthase {ECO:0000255|HAMAP-Rule:MF_00323};
DE   AltName: Full=Protoheme ferro-lyase {ECO:0000255|HAMAP-Rule:MF_00323};
GN   Name=hemH {ECO:0000255|HAMAP-Rule:MF_00323}; OrderedLocusNames=PA4655;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX.
CC       {ECO:0000255|HAMAP-Rule:MF_00323}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + heme b = Fe(2+) + protoporphyrin IX;
CC         Xref=Rhea:RHEA:22584, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033,
CC         ChEBI:CHEBI:57306, ChEBI:CHEBI:60344; EC=4.99.1.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00323};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoheme
CC       biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_00323}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00323}.
CC   -!- SIMILARITY: Belongs to the ferrochelatase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00323, ECO:0000305}.
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DR   EMBL; AE004091; AAG08042.1; -; Genomic_DNA.
DR   PIR; G83064; G83064.
DR   RefSeq; NP_253344.1; NC_002516.2.
DR   RefSeq; WP_003099324.1; NZ_QZGE01000029.1.
DR   AlphaFoldDB; Q9HVD7; -.
DR   SMR; Q9HVD7; -.
DR   STRING; 287.DR97_1964; -.
DR   PaxDb; Q9HVD7; -.
DR   PRIDE; Q9HVD7; -.
DR   DNASU; 881311; -.
DR   EnsemblBacteria; AAG08042; AAG08042; PA4655.
DR   GeneID; 881311; -.
DR   KEGG; pae:PA4655; -.
DR   PATRIC; fig|208964.12.peg.4877; -.
DR   PseudoCAP; PA4655; -.
DR   HOGENOM; CLU_018884_0_1_6; -.
DR   InParanoid; Q9HVD7; -.
DR   OMA; LGDPYHC; -.
DR   PhylomeDB; Q9HVD7; -.
DR   BioCyc; PAER208964:G1FZ6-4751-MON; -.
DR   UniPathway; UPA00252; UER00325.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004325; F:ferrochelatase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006783; P:heme biosynthetic process; IBA:GO_Central.
DR   CDD; cd00419; Ferrochelatase_C; 1.
DR   CDD; cd03411; Ferrochelatase_N; 1.
DR   HAMAP; MF_00323; Ferrochelatase; 1.
DR   InterPro; IPR001015; Ferrochelatase.
DR   InterPro; IPR033644; Ferrochelatase_C.
DR   InterPro; IPR033659; Ferrochelatase_N.
DR   PANTHER; PTHR11108; PTHR11108; 1.
DR   Pfam; PF00762; Ferrochelatase; 1.
DR   TIGRFAMs; TIGR00109; hemH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Heme biosynthesis; Iron; Lyase; Metal-binding;
KW   Porphyrin biosynthesis; Reference proteome.
FT   CHAIN           1..340
FT                   /note="Ferrochelatase"
FT                   /id="PRO_0000175183"
FT   BINDING         189
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00323"
FT   BINDING         292
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00323"
SQ   SEQUENCE   340 AA;  38572 MW;  5AE03FCAD305B86F CRC64;
     MTENALLLLN LGSPDSTRVE DVRRYLDQFL MDPYVVDLPW PLRRLLVSLI LIKRPAESAH
     AYSSIWWDEG SPLIVLSRRL QEAMKPHWPH GPVELAMRYG QPAIEKVLLD LARRGIRRVT
     LAPLYPQFAD STTTTAEQEV RRVIAAHRLG LEVSTLPPFY DQPVYLDALV ESVRPYLQQP
     HDHLLLSFHG LPERHIRKLV KDPAHDLLAE NSRNVSPEAL ALCYRSQCLR TAEAFAERAG
     LEQGRWSVSF QSRLGRAKWI EPYTDAKLDE LVQRGVKRLL VMCPAFVADC IETLEEIGMR
     GREQFISAGG EDLVLIPCLN DHPAWVGALA EMSGRLARPL