HEMOT_DROME
ID HEMOT_DROME Reviewed; 88 AA.
AC A0A0B4K753;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Hemotin {ECO:0000303|PubMed:27015288};
GN Name=hemo {ECO:0000303|PubMed:27015288};
GN ORFNames=CG43194 {ECO:0000312|FlyBase:FBgn0262823};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH 14-3-3ZETA, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=27015288; DOI=10.1371/journal.pbio.1002395;
RA Pueyo J.I., Magny E.G., Sampson C.J., Amin U., Evans I.R., Bishop S.A.,
RA Couso J.P.;
RT "Hemotin, a regulator of phagocytosis encoded by a small ORF and conserved
RT across metazoans.";
RL PLoS Biol. 14:E1002395-E1002395(2016).
CC -!- FUNCTION: Negatively regulates early endosome maturation by binding to
CC and repressing the activity of 14-3-3zeta which prevents the 14-3-
CC 3zeta-mediated activation of phosphoinositide 3-kinase Pi3K68D. This,
CC in turn, inhibits the Pi3K68D-mediated conversion of
CC phosphatidylinositol to phosphatidylinositol-3-phosphate and prevents
CC progression of early endosomes through the maturation process which
CC regulates subsequent steps of phagocytic processing.
CC {ECO:0000269|PubMed:27015288}.
CC -!- SUBUNIT: Interacts with 14-3-3zeta. {ECO:0000269|PubMed:27015288}.
CC -!- SUBCELLULAR LOCATION: Early endosome membrane
CC {ECO:0000269|PubMed:27015288}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in hemocytes.
CC {ECO:0000269|PubMed:27015288}.
CC -!- DEVELOPMENTAL STAGE: Expression increases from prepupal to adult
CC stages. {ECO:0000269|PubMed:27015288}.
CC -!- DISRUPTION PHENOTYPE: Mutants reach adulthood and show no overt
CC morphological defects but their hemocytes display a more vacuolated
CC morphology than controls with enlarged endolysosomes which accumulate
CC undigested phagocytic material due to impaired digestion. This leads to
CC decreased resistance to bacterial infection and severely reduced
CC lifespan. {ECO:0000269|PubMed:27015288}.
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DR EMBL; AE014297; AFH06498.1; -; Genomic_DNA.
DR EMBL; AE014297; AHN57392.1; -; Genomic_DNA.
DR RefSeq; NP_001247180.1; NM_001260251.2.
DR RefSeq; NP_001287393.1; NM_001300464.1.
DR AlphaFoldDB; A0A0B4K753; -.
DR STRING; 7227.FBpp0297277; -.
DR PaxDb; A0A0B4K753; -.
DR EnsemblMetazoa; FBtr0306140; FBpp0297277; FBgn0262823.
DR EnsemblMetazoa; FBtr0346523; FBpp0312141; FBgn0262823.
DR GeneID; 12798465; -.
DR KEGG; dme:Dmel_CG43194; -.
DR CTD; 12798465; -.
DR FlyBase; FBgn0262823; hemo.
DR VEuPathDB; VectorBase:FBgn0262823; -.
DR OMA; CPHIINT; -.
DR PhylomeDB; A0A0B4K753; -.
DR BioGRID-ORCS; 12798465; 0 hits in 1 CRISPR screen.
DR PRO; PR:A0A0B4K753; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0262823; Expressed in embryonic hemocyte (Drosophila) and 6 other tissues.
DR GO; GO:0031901; C:early endosome membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006909; P:phagocytosis; IMP:FlyBase.
DR GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:1904980; P:positive regulation of endosome organization; IMP:UniProtKB.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Endosome; Membrane; Phagocytosis; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..88
FT /note="Hemotin"
FT /id="PRO_0000436375"
FT TOPO_DOM 1..14
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 15..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
SQ SEQUENCE 88 AA; 10050 MW; 9B7C8A8B5B25D83F CRC64;
MDCFKVFEVV FQSEINPLLL IPAVATIALT LCCYCYHGYQ WIRDRRTARI EEQQAQLPLP
LSRISITPGC SMVATTKLTH SRNSVDIY
