ID   HEMX_ECOLI              Reviewed;         393 AA.
AC   P09127; Q2M8A8;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=Protein HemX {ECO:0000305};
DE   AltName: Full=ORF X {ECO:0000312|EMBL:CAA31134.1};
GN   Name=hemX {ECO:0000303|PubMed:3062586}; OrderedLocusNames=b3803, JW3775;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=3062586; DOI=10.1093/nar/16.24.11835;
RA   Sasarman A., Echelard Y., Letowski J., Tardif D., Drolet M.;
RT   "Nucleotide sequence of the hemX gene, the third member of the Uro operon
RT   of Escherichia coli K12.";
RL   Nucleic Acids Res. 16:11835-11835(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / CS520;
RX   PubMed=3054815; DOI=10.1093/nar/16.20.9871;
RA   Alefounder P.R., Abell C., Battersby A.R.;
RT   "The sequence of hemC, hemD and two additional E. coli genes.";
RL   Nucleic Acids Res. 16:9871-9871(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT   84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-12.
RC   STRAIN=K12 / EMG2;
RX   PubMed=9298646; DOI=10.1002/elps.1150180807;
RA   Link A.J., Robison K., Church G.M.;
RT   "Comparing the predicted and observed properties of proteins encoded in the
RT   genome of Escherichia coli K-12.";
RL   Electrophoresis 18:1259-1313(1997).
RN   [7]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
RN   [8]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=BL21-DE3;
RX   PubMed=16079137; DOI=10.1074/jbc.m506479200;
RA   Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L.,
RA   von Heijne G., Daley D.O.;
RT   "Protein complexes of the Escherichia coli cell envelope.";
RL   J. Biol. Chem. 280:34409-34419(2005).
CC   -!- SUBUNIT: Homooligomer, possibly a homopentamer.
CC       {ECO:0000269|PubMed:16079137}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:16079137}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- MISCELLANEOUS: Was suggested to be a uroporphyrinogen-III
CC       methyltransferase, but the function of the protein has not been
CC       experimentally determined. {ECO:0000305|PubMed:3062586}.
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DR   EMBL; X13406; CAA31772.1; -; Genomic_DNA.
DR   EMBL; X12614; CAA31134.1; -; Genomic_DNA.
DR   EMBL; M87049; AAA67599.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76806.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77498.1; -; Genomic_DNA.
DR   PIR; S02185; S02185.
DR   RefSeq; NP_418247.1; NC_000913.3.
DR   RefSeq; WP_000138997.1; NZ_SSZK01000025.1.
DR   AlphaFoldDB; P09127; -.
DR   SMR; P09127; -.
DR   BioGRID; 4259707; 160.
DR   DIP; DIP-9888N; -.
DR   IntAct; P09127; 3.
DR   STRING; 511145.b3803; -.
DR   jPOST; P09127; -.
DR   PaxDb; P09127; -.
DR   PRIDE; P09127; -.
DR   EnsemblBacteria; AAC76806; AAC76806; b3803.
DR   EnsemblBacteria; BAE77498; BAE77498; BAE77498.
DR   GeneID; 948446; -.
DR   KEGG; ecj:JW3775; -.
DR   KEGG; eco:b3803; -.
DR   PATRIC; fig|1411691.4.peg.2905; -.
DR   EchoBASE; EB0428; -.
DR   eggNOG; COG2959; Bacteria.
DR   HOGENOM; CLU_036381_1_0_6; -.
DR   InParanoid; P09127; -.
DR   OMA; QAIPRHQ; -.
DR   PhylomeDB; P09127; -.
DR   BioCyc; EcoCyc:HEMX-MON; -.
DR   PRO; PR:P09127; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007470; HemX.
DR   PANTHER; PTHR38043; PTHR38043; 1.
DR   Pfam; PF04375; HemX; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Direct protein sequencing; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..393
FT                   /note="Protein HemX"
FT                   /id="PRO_0000135255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          366..393
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        14..30
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   393 AA;  42963 MW;  9D272C6401D0E354 CRC64;
     MTEQEKTSAV VEETREAVDT TSQPVATEKK SKNNTALILS AVAIAIALAA GIGLYGWGKQ
     QAVNQTATSD ALANQLTALQ KAQESQKAEL EGIIKQQAAQ LKQANRQQET LAKQLDEVQQ
     KVATISGSDA KTWLLAQADF LVKLAGRKLW SDQDVTTAAA LLKSADASLA DMNDPSLITV
     RRAITDDIAS LSAVSQVDYD GIILKLNQLS NQVDNLRLAD NDSDGSPMDS DGEELSSSIS
     EWRINLQKSW QNFMDNFITI RRRDDTAVPL LAPNQDIYLR ENIRSRLLVA AQAVPRHQEE
     TYRQALENVS TWVRAYYDTD DATTKAFLDE VDQLSQQNIS MDLPETLQSQ AMLEKLMQTR
     VRNLLAQPAA GTTEAKPAPA PQADTPAAAP QGE