HEN4_ARATH
ID HEN4_ARATH Reviewed; 857 AA.
AC F4KDN0; Q9FMF0;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=KH domain-containing protein HEN4 {ECO:0000305};
DE AltName: Full=Protein HUA ENHANCER 4 {ECO:0000303|PubMed:15310842};
GN Name=HEN4 {ECO:0000303|PubMed:15310842};
GN OrderedLocusNames=At5g64390 {ECO:0000312|Araport:AT5G64390};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, INTERACTION WITH HUA1, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=15310842; DOI=10.1073/pnas.0404552101;
RA Mockler T.C., Yu X., Shalitin D., Parikh D., Michael T.P., Liou J.,
RA Huang J., Smith Z., Alonso J.M., Ecker J.R., Chory J., Lin C.;
RT "Regulation of flowering time in Arabidopsis by K homology domain
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12759-12764(2004).
RN [5]
RP INTERACTION WITH FLK AND PEP.
RX PubMed=25658099; DOI=10.1371/journal.pgen.1004983;
RA Rodriguez-Cazorla E., Ripoll J.J., Andujar A., Bailey L.J.,
RA Martinez-Laborda A., Yanofsky M.F., Vera A.;
RT "K-homology nuclear ribonucleoproteins regulate floral organ identity and
RT determinacy in arabidopsis.";
RL PLoS Genet. 11:E1004983-E1004983(2015).
CC -!- FUNCTION: Functions in floral reproductive organ identity in the third
CC whorl and floral determinacy specification by specifically promoting
CC the processing of AGAMOUS (AG) pre-mRNA. Functions in association with
CC HUA1 and HUA2. {ECO:0000269|PubMed:15310842}.
CC -!- SUBUNIT: Interacts with HUA1 (PubMed:15310842). Interacts with FLK and
CC PEP (PubMed:25658099). {ECO:0000269|PubMed:15310842,
CC ECO:0000269|PubMed:25658099}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:15310842}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=F4KDN0-1; Sequence=Displayed;
CC -!- DEVELOPMENTAL STAGE: Expressed in inflorescence meristem during flower
CC development at least until stage 6 of development.
CC {ECO:0000269|PubMed:15310842}.
CC -!- DISRUPTION PHENOTYPE: Floral organ defects such as reproductive-to-
CC perianth organ transformation and loss of floral determinacy.
CC {ECO:0000269|PubMed:15310842}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09870.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BT004044; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB008268; BAB09870.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97884.1; -; Genomic_DNA.
DR EMBL; BT004044; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_201244.2; NM_125835.5. [F4KDN0-1]
DR AlphaFoldDB; F4KDN0; -.
DR SMR; F4KDN0; -.
DR STRING; 3702.AT5G64390.3; -.
DR iPTMnet; F4KDN0; -.
DR ProteomicsDB; 230273; -. [F4KDN0-1]
DR EnsemblPlants; AT5G64390.1; AT5G64390.1; AT5G64390. [F4KDN0-1]
DR GeneID; 836560; -.
DR Gramene; AT5G64390.1; AT5G64390.1; AT5G64390. [F4KDN0-1]
DR KEGG; ath:AT5G64390; -.
DR Araport; AT5G64390; -.
DR eggNOG; KOG2190; Eukaryota.
DR OMA; CKIAIRT; -.
DR PRO; PR:F4KDN0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KDN0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0048497; P:maintenance of floral organ identity; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR Gene3D; 3.30.1370.10; -; 3.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR Pfam; PF00013; KH_1; 5.
DR SMART; SM00322; KH; 5.
DR SUPFAM; SSF54791; SSF54791; 5.
DR PROSITE; PS50084; KH_TYPE_1; 5.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Flowering;
KW mRNA processing; Nucleus; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..857
FT /note="KH domain-containing protein HEN4"
FT /id="PRO_0000436751"
FT DOMAIN 46..110
FT /note="KH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 149..217
FT /note="KH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 451..521
FT /note="KH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 541..610
FT /note="KH 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 775..839
FT /note="KH 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 644..755
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 644..688
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 857 AA; 93693 MW; FE285CC9C7A51616 CRC64;
MERNSVKFHA EKRSGAFDPG SGFGSSKRVK THHTQLLSAL VVPVGHAAFR LLCPLSHVGA
VIGKSGNVIK QLQQSTGAKI RVEEPPSGSP DRVITIIAQA DSKSRVKLGA NNNGNAEGEK
KEEEVEVSKA QGALIKVFEL LAAEADSDTV VCRLLTESSH AGAVIGKGGQ MVGSIRKETG
CKISIRIENL PICADTDDEM VEVEGNAIAV KKALVSISRC LQNCQSIDKV RMVGNRPLEK
EFQASLHRPI ETIIQESLPR SVEVNPYDYR LRNDEIFPRG TVARANDVIP HDTLHLRRIE
AVPQGALRMH IEADRQDVLR RHVEADRQDA LRRRIDVVPQ ETLYMPSDVL RGDCFRQHRE
RDDSHDSLHR PFEMVPRDAM GMPFESFPRD AYGRPIETMT QETLRGQSAD YLAHRYSTLD
THPHSFTTSA SMANTATMKP PPSEVEVGNQ DVVFKILCST ENAGGVIGTG GKVVRMLHSE
TGAFINVGNT LDDCEERLIA VTASENPECQ SSPAQKAIML IFSRLFELAT NKILDNGPRS
SITARLVVPT SQIGCVLGKG GVIVSEMRKT TGAAIQILKV EQNPKCISEN DQVVQITGEF
PNVREAIFHI TSRLRDSVFS NSMKNSLAKS SSALTTERFY DRQSDNPLSI GSHQSVSNPA
TNSSSLHRRS EDSFLSGSHS SVNYSRSVGT DPYIRPEDPF PDRFNPSAGY SHNFGRRFTM
DHSDNSHHLT EAPSRLWASP PPAAPRGLSD ASGGLSSARA GHVLGSGHKS AIVTNTTVEI
RVPANAMSFV YGEQGYNLEQ LRQISGARVI IHEPPLGTSD RIIVISGTPD QTQAAQNLLH
AFILTGETSL SKKYNLN
