HEPA_HHV11
ID HEPA_HHV11 Reviewed; 750 AA.
AC P10192; B9VQD5; Q09IC5;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 23-FEB-2022, entry version 85.
DE RecName: Full=DNA helicase/primase complex-associated protein {ECO:0000255|HAMAP-Rule:MF_04010};
DE Short=HEPA {ECO:0000255|HAMAP-Rule:MF_04010};
DE AltName: Full=Primase-associated factor {ECO:0000255|HAMAP-Rule:MF_04010};
GN ORFNames=UL8;
OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX NCBI_TaxID=10299;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA Perry L.J., Scott J.E., Taylor P.;
RT "The complete DNA sequence of the long unique region in the genome of
RT herpes simplex virus type 1.";
RL J. Gen. Virol. 69:1531-1574(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2826807; DOI=10.1128/jvi.62.2.444-453.1988;
RA McGeoch D.J., Dalrymple M.A., Dolan A., McNab D., Perry L.J., Taylor P.,
RA Challberg M.D.;
RT "Structures of herpes simplex virus type 1 genes required for replication
RT of virus DNA.";
RL J. Virol. 62:444-453(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nonneuroinvasive mutant HF10;
RX PubMed=17218138; DOI=10.1016/j.micinf.2006.10.019;
RA Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.;
RT "Determination and analysis of the DNA sequence of highly attenuated herpes
RT simplex virus type 1 mutant HF10, a potential oncolytic virus.";
RL Microbes Infect. 9:142-149(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17 syn+;
RA Cunningham C., Davison A.J.;
RT "Herpes simplex virus type 1 bacterial artificial chromosome.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH UL9, AND FUNCTION.
RX PubMed=7931156; DOI=10.1099/0022-1317-75-10-2699;
RA McLean G.W., Abbotts A.P., Parry M.E., Marsden H.S., Stow N.D.;
RT "The herpes simplex virus type 1 origin-binding protein interacts
RT specifically with the viral UL8 protein.";
RL J. Gen. Virol. 75:2699-2706(1994).
RN [6]
RP INTERACTION WITH UL30, AND FUNCTION.
RX PubMed=9261356; DOI=10.1128/jvi.71.9.6390-6397.1997;
RA Marsden H.S., McLean G.W., Barnard E.C., Francis G.J., MacEachran K.,
RA Murphy M., McVey G., Cross A., Abbotts A.P., Stow N.D.;
RT "The catalytic subunit of the DNA polymerase of herpes simplex virus type 1
RT interacts specifically with the C terminus of the UL8 component of the
RT viral helicase-primase complex.";
RL J. Virol. 71:6390-6397(1997).
CC -!- FUNCTION: Component of the helicase/primase complex. Unwinds the DNA at
CC the replication forks and generates single-stranded DNA for both
CC leading and lagging strand synthesis. The primase synthesizes short RNA
CC primers on the lagging strand that the polymerase presumably elongates
CC using dNTPs. The primase-associated factor has no known catalytic
CC activity in the complex and may serve to facilitate the formation of
CC the replisome by directly interacting with the origin-binding protein
CC and the polymerase. {ECO:0000255|HAMAP-Rule:MF_04010,
CC ECO:0000269|PubMed:7931156, ECO:0000269|PubMed:9261356}.
CC -!- SUBUNIT: Associates with the primase and the helicase to form the
CC helicase-primase complex. Interacts with the origin-binding protein.
CC Interacts with the polymerase catalytic subunit. {ECO:0000255|HAMAP-
CC Rule:MF_04010, ECO:0000269|PubMed:7931156, ECO:0000269|PubMed:9261356}.
CC -!- INTERACTION:
CC P10192; P04293: UL30; NbExp=4; IntAct=EBI-7185538, EBI-8615017;
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04010}.
CC -!- SIMILARITY: Belongs to the herpesviridae HEPA family.
CC {ECO:0000255|HAMAP-Rule:MF_04010}.
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DR EMBL; X14112; CAA32344.1; -; Genomic_DNA.
DR EMBL; M19120; AAA45823.1; -; Genomic_DNA.
DR EMBL; DQ889502; ABI63470.1; -; Genomic_DNA.
DR EMBL; FJ593289; ACM62230.1; -; Genomic_DNA.
DR PIR; C29890; WMBEX8.
DR RefSeq; YP_009137082.1; NC_001806.2.
DR BioGRID; 971456; 1.
DR DIP; DIP-1097N; -.
DR IntAct; P10192; 2.
DR MINT; P10192; -.
DR ChEMBL; CHEMBL4630721; -.
DR PRIDE; P10192; -.
DR GeneID; 2703432; -.
DR KEGG; vg:2703432; -.
DR Proteomes; UP000009294; Genome.
DR Proteomes; UP000180652; Genome.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IDA:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR HAMAP; MF_04010; HSV_HEPA; 1.
DR InterPro; IPR004996; HSV_HEPA.
DR Pfam; PF03324; Herpes_HEPA; 1.
PE 1: Evidence at protein level;
KW DNA replication; Host nucleus; Reference proteome.
FT CHAIN 1..750
FT /note="DNA helicase/primase complex-associated protein"
FT /id="PRO_0000115856"
FT VARIANT 247
FT /note="T -> M (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 330
FT /note="A -> T (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 353
FT /note="A -> V (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 467
FT /note="N -> D (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 471
FT /note="A -> V (in strain: Nonneuroinvasive mutant HF10)"
FT VARIANT 543..544
FT /note="AV -> GL (in strain: Nonneuroinvasive mutant HF10
FT and 17 syn+)"
FT VARIANT 612
FT /note="C -> G (in strain: Nonneuroinvasive mutant HF10)"
SQ SEQUENCE 750 AA; 79926 MW; ECA9ABD0E85CB392 CRC64;
MDTADIVWVE ESVSAITLYA VWLPPRAREY FHALVYFVCR NAAGEGRARF AEVSVTATEL
RDFYGSADVS VQAVVAAARA ATTPAASPLE PLENPTLWRA LYACVLAALE RQTGPVALFA
PLRIGSDPRT GLVVKVERAS WGPPAAPRAA LLVAEANIDI DPMALAARVA EHPDARLAWA
RLAAIRDTPQ CASAASLTVN ITTGTALFAR EYQTLAFPPI KKEGAFGDLV EVCEVGLRPR
GHPQRVTARV LLPRDYDYFV SAGEKFSAPA LVALFRQWHT TVHAAPGALA PVFAFLGPEF
EVRGGPVPYF AVLGFPGWPT FTVPATAESA RDLVRGAAAA YAALLGAWPA VGARVVLPPR
AWPGVASAAA GCLLPAVREA VARWHPATKI IQLLDPPAAV GPVWTARFCF PGLRAQLLAA
LADLGGSGLA DPHGRTGLAR LDALVVAAPS EPWAGAVLER LVPDTCNACP ALRQLLGGVM
AAVCLQIEET ASSVKFAVCG GDGGAFWGVF NVDPQDADAA SGVIEDARRA IETAVGAVLR
ANAVRLRHPL CLALEGVYTH AVAWSQAGVW FWNSRDNTDH LGGFPLRGPA YTTAAGVVRD
TLRRVLGLTT ACVPEEDALT ARGLMEDACD RLILDAFNKR LDAEYWSVRV SPFEASDPLP
PTAFRGGALL DAEHYWRRVV RVCPGGGESV GVPVDLYPRP LVLPPVDCAH HLREILREIE
LVFTGVLAGV WGEGGKFVYP FDDKMSFLFA
