ID   HEPA_VZVO               Reviewed;         771 AA.
AC   Q9J3N6; Q4JQS3; Q4JR32;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=DNA helicase/primase complex-associated protein {ECO:0000255|HAMAP-Rule:MF_04010};
DE            Short=HEPA {ECO:0000255|HAMAP-Rule:MF_04010};
DE   AltName: Full=Primase-associated factor {ECO:0000255|HAMAP-Rule:MF_04010};
GN   ORFNames=ORF52;
OS   Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=341980;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oka varicella vaccine Biken (V-Oka-Biken);
RX   PubMed=10720545; DOI=10.1086/315335;
RA   Argaw T., Cohen J.I., Klutch M., Lekstrom K., Yoshikawa T., Asano Y.,
RA   Krause P.R.;
RT   "Nucleotide sequences that distinguish Oka vaccine from parental Oka and
RT   other varicella-zoster virus isolates.";
RL   J. Infect. Dis. 181:1153-1157(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oka varicella vaccine VarilRix (V-Oka-GSK), and
RC   Oka varicella vaccine Varivax (V-Oka-Merk);
RX   PubMed=18787000; DOI=10.1128/jvi.00777-08;
RA   Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M.,
RA   Vassilev V.;
RT   "Complete DNA sequences of two oka strain varicella-zoster virus genomes.";
RL   J. Virol. 82:11023-11044(2008).
CC   -!- FUNCTION: Component of the helicase/primase complex. Unwinds the DNA at
CC       the replication forks and generates single-stranded DNA for both
CC       leading and lagging strand synthesis. The primase synthesizes short RNA
CC       primers on the lagging strand that the polymerase presumably elongates
CC       using dNTPs. The primase-associated factor has no known catalytic
CC       activity in the complex and may serve to facilitate the formation of
CC       the replisome by directly interacting with the origin-binding protein
CC       and the polymerase. {ECO:0000255|HAMAP-Rule:MF_04010}.
CC   -!- SUBUNIT: Associates with the primase and the helicase to form the
CC       helicase-primase complex. Interacts with the origin-binding protein.
CC       Interacts with the polymerase catalytic subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_04010}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04010}.
CC   -!- SIMILARITY: Belongs to the herpesviridae HEPA family.
CC       {ECO:0000255|HAMAP-Rule:MF_04010}.
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DR   EMBL; AF206304; AAF61653.1; -; Genomic_DNA.
DR   EMBL; DQ008355; AAY57732.1; -; Genomic_DNA.
DR   EMBL; DQ008354; AAY57661.1; -; Genomic_DNA.
DR   IntAct; Q9J3N6; 3.
DR   MINT; Q9J3N6; -.
DR   ChEMBL; CHEMBL4523677; -.
DR   PRIDE; Q9J3N6; -.
DR   Proteomes; UP000008504; Genome.
DR   Proteomes; UP000008506; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_04010; HSV_HEPA; 1.
DR   InterPro; IPR004996; HSV_HEPA.
DR   Pfam; PF03324; Herpes_HEPA; 1.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus.
FT   CHAIN           1..771
FT                   /note="DNA helicase/primase complex-associated protein"
FT                   /id="PRO_0000385139"
FT   VARIANT         512
FT                   /note="T -> A"
SQ   SEQUENCE   771 AA;  86337 MW;  DBC410E005920722 CRC64;
     MDATQITLVR ESGHICAASI YTSWTQSGQL TQNGLSVLYY LLCKNSCGKY VPKFAEITVQ
     QEDLCRYSRH GGSVSAATFA SICRAASSAA LDAWPLEPLG NADTWRCLHG TALATLRRVL
     GFKSFYSPVT FETDTNTGLL LKTIPDEHAL NNDNTPSTGV LRANFPVAID VSAVSACNAH
     TQGTSLAYAR LTALKSNGDT QQQTPLDVEV ITPKAYIRRK YKSTFSPPIE REGQTSDLFN
     LEERRLVLSG NRAIVVRVLL PCYFDCLTTD STVTSSLSIL ATYRLWYAAA FGKPGVVRPI
     FAYLGPELNP KGEDRDYFCT VGFPGWTTLR TQTPAVESIR TATEMYMETD GLWPVTGIQA
     FHYLAPWGQH PPLPPRVQDL IGQIPQDTGH ADATVNWDAG RISTVFKQPV QLQDRWMAKF
     DFSAFFPTIY CAMFPMHFRL GKIVLARMRR GMGCLKPALV SFFGGLRHIL PSIYKAIIFI
     ANEISLCVEQ TALEQGFAIC TYIKDGFWGI FTDLHTRNVC SDQARCSALN LAAACERAVT
     GLLRIQLGLN FTPAMEPVLR VEGVYTHAFT WCTTGSWLWN LQTNTPPDLV GVPWRSQAAR
     DLKERLSGLL CTATKIRERI QENCIWDRVL YDIWAGQVVE AARKTYVDFF EHVFDRRYTP
     VYWSLQEQNS ETKAIPASYL TYGHMQDKDY KPRQIIMVRN PNPHGPPTVV YWELLPSCAC
     IPPIDCAAHL KPLIHTFVTI INHLLDAHND FSSPSLKFTD DPLASYNFLF L