HEPF1_PLAF7
ID HEPF1_PLAF7 Reviewed; 2082 AA.
AC C0H4W3;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Probable ATP-dependent helicase PF08_0048;
DE EC=3.6.4.-;
GN ORFNames=PF08_0048;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3] {ECO:0000305}
RP SYNTHESIS OF 284-312, AND POSSIBLE CANDIDATE MALARIA EPITOPE.
RX PubMed=17653272; DOI=10.1371/journal.pone.0000645;
RA Villard V., Agak G.W., Frank G., Jafarshad A., Servis C., Nebie I.,
RA Sirima S.B., Felger I., Arevalo-Herrera M., Herrera S., Heitz F.,
RA Baecker V., Druilhe P., Kajava A.V., Corradin G.;
RT "Rapid identification of malaria vaccine candidates based on alpha-helical
RT coiled coil protein motif.";
RL PLoS ONE 2:E645-E645(2007).
CC -!- FUNCTION: Catalytic component of a chromatin remodeling complex.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of a chromatin-remodeling complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00549}.
CC -!- BIOTECHNOLOGY: Possible candidate for an effective malaria vaccine as
CC determined by epitope response in sera. {ECO:0000269|PubMed:17653272}.
CC -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
CC {ECO:0000255}.
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DR EMBL; AL844507; CAX64141.1; -; Genomic_DNA.
DR RefSeq; XP_002808863.1; XM_002808817.1.
DR AlphaFoldDB; C0H4W3; -.
DR SMR; C0H4W3; -.
DR BioGRID; 1210343; 7.
DR STRING; 5833.PF08_0048; -.
DR PRIDE; C0H4W3; -.
DR EnsemblProtists; CAX64141; CAX64141; PF3D7_0820000.
DR GeneID; 2655499; -.
DR KEGG; pfa:PF3D7_0820000; -.
DR VEuPathDB; PlasmoDB:PF3D7_0820000; -.
DR HOGENOM; CLU_001568_0_0_1; -.
DR InParanoid; C0H4W3; -.
DR OMA; TSYCFNF; -.
DR PhylomeDB; C0H4W3; -.
DR Proteomes; UP000001450; Chromosome 8.
DR GO; GO:0005737; C:cytoplasm; NAS:UniProtKB.
DR GO; GO:0016020; C:membrane; NAS:UniProtKB.
DR GO; GO:0000812; C:Swr1 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; ISS:GeneDB.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; ISS:GeneDB.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0043486; P:histone exchange; IBA:GO_Central.
DR Gene3D; 3.40.50.10810; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR014012; HSA_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038718; SNF2-like_sf.
DR InterPro; IPR000330; SNF2_N.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07529; HSA; 1.
DR Pfam; PF00176; SNF2-rel_dom; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 3.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51204; HSA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chromatin regulator; Coiled coil; DNA-binding; Helicase;
KW Hydrolase; Merozoite; Nucleotide-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..2082
FT /note="Probable ATP-dependent helicase PF08_0048"
FT /id="PRO_0000388752"
FT DOMAIN 66..138
FT /note="HSA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00549"
FT DOMAIN 674..839
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 1772..1922
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 209..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 301..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1199..1255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 476..531
FT /evidence="ECO:0000255"
FT COILED 1972..2060
FT /evidence="ECO:0000255"
FT MOTIF 790..793
FT /note="DEAH box"
FT /evidence="ECO:0000255"
FT COMPBIAS 301..329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..443
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..470
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..574
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 575..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 599..624
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..641
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 687..694
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 2082 AA; 246748 MW; 253B2FB6DDCFD602 CRC64;
MNEIKSESLL QTRPFKLGIE DIQNLGSSYF IENNEKLKKY NNEISSLKKE LDILNEKMGK
CTTTTKIVEP AKTPEFTFWY YELKEMKGFQ DLVMYEVKKK KKHFKVLSHS CLKYLSNREK
MKIKKQEEEE KRLKLYSKNI SSYMDVFWKK IEKLVWEEKK RELQQTLNKK KEMRFKKFVK
EAIKKIKDAR HNNAHELFEN KYVSMSSNNN SEIVNNNASS VDNGDKELKE DDLTDQEEED
YLLDEQMSST DESENKEEEI NMLDDEANLP IEELLKRMYG FKSGEDYINF MENEDDANEE
NVIETSHNDE KSGDNSIGED DNNNDEKGGD NNIDEDDNNN DEKSGDNSIG EDDNNNDHKS
GDNNIDEDDN NNDHKSEDNS IGEDDNNNDE KGGDNNIDEN DNNSDHKSED NNIDENDNNS
DHQSDQEQFN HETKDDIIKN SSYEHIDNKN YYNKTGEDYK SDKENYSPTR FHNKLKKEKY
DEYDTKLKIE KREEENKNYE KDEHEYESDN YDKEKINKKK ELILLKNDIE NDSDETSEHI
KRDSRSSCQK QNCEKKRRII KDEYNLRRTK IAKSKPSSDN NNSENDNNND NNNDNNNDNN
DDNNDDNNDD NNDDNNDDNN DDNNDDNNNE HKNDSDDNDD ILTCNMDEKH LTKIPPIIKA
TLRDYQHAGL HWLLYLYKNN INGILADEMG LGKTLQCISL LSYLAYYFNI WGPHLVIVPT
SILINWEIEL KRFCPCFKIL SYYGNQNERY KKRVGWFNKD SFHICISSYS TVVKDHLVFK
RKRWKYIILD EAHNIKNFNT KRWNIILSLK RDNCLLITGT PLQNSLEELW SLLHFLMPNI
FTSHLDFKEW FSDPLNLAIE KSKIHHSKEL IDRLHTVIRP YILRRLKKNV EKEMPNKYEH
IIKCKLTRRQ QILYDEFINN KNVQNTLNTG NYIGLMNILI QLRKVCNHCD LFTNKYIQTP
YYYMLSIRYF VPRFFILFEK NYYADFYLIL FLHNEFTSLG GRDVTKETSP SSKSFDLAHI
LTKHNTNELY DNNHISELYD NNHISELYDN NHISELYDNN HISELYDNPM SHKNYKHNSN
GYTYPNDPIN NMNNNPSGFT KTSEQFGQIV SHERDNNYHM MDHNNMNNLL SKEMVNSLRN
DDNSNNNFYK YSLTSNNNDS QTSIHDNKQC DYNKLCADTF NNINSIGNEE KRSLNVLNEQ
NNNNSKDNNN NIDNNNNIDN NNNIDNNNNI DNNNNIDNNN NNIDNNNNID NHHNNNQHCN
YNDNWPSDYP TNIINHRNAF LSILKLLNQS NPLNNDNNNN NNNNNGNNNI YNMNRYNSRN
SRNSSLSNIF SSNTSKMNSF QLDFLYTNSF INQDALCKNS FFVNINIEDV HSYIYNSIYK
EYIPKNILSF SDEFLTELNN NYDILSLYID PYNRYKSYNE YLYKMKEEGT LTNQQSLGDI
NNKHIYHKST SNENTHMKNR KTFIYKYNNM FKVINNDTQY QNIFTDDTNN SYYNSLEHNL
WIKRNQIDER KKEEEEEQNK YYNVCMNNLY ILRNERIPIF GKNFLDLIKK EFTKDKNIVY
NYTNNVPIDY YSSVKEVWVE DICEKDNKKR KCKREKRWYK KIKKTNNPPE DSEVYRENSS
DVEKYNCDVE KDNCDDEEKD NCDDEDMNSN LSSNVYGCID ISSQNFIHSR YHNPMMNMSY
IIEFLFPNME QFLKRHEKMI HNFTLINNPS VICKSHDIRI NNNLLNYSND KMNPIILQIK
NATRVYHDAF LKQSIIFPLN KDISLGSGKL CALEKLLSKC KREGNKCLLF TQFIKMLDIL
EIFLNHLNYS FIRLDGSTKV EQRQKIVTKF NNDKSIFIFI SSTRSGSIGI NLTAANVVIF
YDTDWNPSID KQAMDRCHRI GQTKDVHVFR FVCEYTVEEN IWKKQLQKRK LDNICINMGN
FNNSNTHSKI TDTDPTHNKD WFTNVDTIKE VFINKKNNDD DDDMYKDRLL HEQVENKDKM
NVRFEKTLEH VEDKDDIRAL NETKKETQNE ISQNMQEFTT RNDFQDSYNL TSYCFNFLNE
NLTDSLKQQI DEMRMKIEIE MMNTGDENMS LSDLSNKSHN SE
