ID   HERC4_RAT               Reviewed;        1057 AA.
AC   Q5PQN1;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Probable E3 ubiquitin-protein ligase HERC4;
DE            EC=2.3.2.26;
DE   AltName: Full=HECT domain and RCC1-like domain-containing protein 4;
DE   AltName: Full=HECT-type E3 ubiquitin transferase HERC4;
GN   Name=Herc4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Probable E3 ubiquitin-protein ligase involved in either
CC       protein trafficking or in the distribution of cellular structures.
CC       Required for spermatozoon maturation and fertility, and for the removal
CC       of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein
CC       ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the
CC       form of a thioester and then directly transfer it to targeted
CC       substrates. {ECO:0000250|UniProtKB:Q6PAV2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q5GLZ8}.
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DR   EMBL; BC087104; AAH87104.1; -; mRNA.
DR   RefSeq; NP_001012074.1; NM_001012074.1.
DR   RefSeq; XP_006256196.1; XM_006256134.3.
DR   RefSeq; XP_017457145.1; XM_017601656.1.
DR   AlphaFoldDB; Q5PQN1; -.
DR   SMR; Q5PQN1; -.
DR   IntAct; Q5PQN1; 1.
DR   STRING; 10116.ENSRNOP00000049495; -.
DR   iPTMnet; Q5PQN1; -.
DR   PhosphoSitePlus; Q5PQN1; -.
DR   jPOST; Q5PQN1; -.
DR   PaxDb; Q5PQN1; -.
DR   PRIDE; Q5PQN1; -.
DR   Ensembl; ENSRNOT00000083017; ENSRNOP00000072219; ENSRNOG00000061040.
DR   GeneID; 309758; -.
DR   KEGG; rno:309758; -.
DR   UCSC; RGD:1310971; rat.
DR   CTD; 26091; -.
DR   RGD; 1310971; Herc4.
DR   eggNOG; KOG0941; Eukaryota.
DR   GeneTree; ENSGT00940000158924; -.
DR   HOGENOM; CLU_002173_5_3_1; -.
DR   InParanoid; Q5PQN1; -.
DR   OMA; TIPFAKC; -.
DR   OrthoDB; 1062377at2759; -.
DR   PhylomeDB; Q5PQN1; -.
DR   TreeFam; TF315189; -.
DR   Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q5PQN1; -.
DR   Proteomes; UP000002494; Chromosome 20.
DR   Bgee; ENSRNOG00000061040; Expressed in duodenum and 20 other tissues.
DR   Genevisible; Q5PQN1; RN.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0001650; C:fibrillar center; IEA:Ensembl.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0045879; P:negative regulation of smoothened signaling pathway; ISO:RGD.
DR   GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 2.130.10.30; -; 2.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   InterPro; IPR009091; RCC1/BLIP-II.
DR   InterPro; IPR000408; Reg_chr_condens.
DR   Pfam; PF00632; HECT; 1.
DR   Pfam; PF00415; RCC1; 7.
DR   PRINTS; PR00633; RCCNDNSATION.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF50985; SSF50985; 1.
DR   SUPFAM; SSF56204; SSF56204; 1.
DR   PROSITE; PS50237; HECT; 1.
DR   PROSITE; PS00626; RCC1_2; 3.
DR   PROSITE; PS50012; RCC1_3; 7.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Differentiation; Reference proteome; Repeat; Spermatogenesis;
KW   Transferase; Ubl conjugation pathway.
FT   CHAIN           1..1057
FT                   /note="Probable E3 ubiquitin-protein ligase HERC4"
FT                   /id="PRO_0000278218"
FT   REPEAT          1..51
FT                   /note="RCC1 1"
FT   REPEAT          52..101
FT                   /note="RCC1 2"
FT   REPEAT          102..154
FT                   /note="RCC1 3"
FT   REPEAT          156..207
FT                   /note="RCC1 4"
FT   REPEAT          208..259
FT                   /note="RCC1 5"
FT   REPEAT          261..311
FT                   /note="RCC1 6"
FT   REPEAT          313..366
FT                   /note="RCC1 7"
FT   DOMAIN          730..1057
FT                   /note="HECT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
FT   ACT_SITE        1025
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   1057 AA;  118540 MW;  4A3C6F2B2BABF39B CRC64;
     MLCWGNASYG QLGLGGIDEE IVLEPRKSDF FVNKKVRDVG CGLRHTVFVL DDGTVYTCGC
     NDLGQLGHEK SRKKPEQVVA LDAQNIVAVS CGEAHTLALN DKGQVYAWGL DSDGQLGLQG
     SEECIRVPRN IKSLSDIQIV QVACGYYHSL ALSKASEVFC WGQNKYGQLG LGIECQKQTS
     PQLIKSLLGI PFMQVAAGGA HSFVLTLSGA IFGWGRNKFG QLGLNDENDR YVPNLLKSLR
     SQKIVYICCG EDHTAALTKE GGVFTFGAGG YGQLGHNSTS HEINPRKVFE LMGSIVTQIA
     CGRQHTSAFV PSSGRIYSFG LGGNGQLGTG STSNRKSPFT VKGNWFSYNG QCPQDIGSED
     YFCVKRIFSG GDQSFSHYSS PQSCGPPDDF RRSDPSKQIW TVNEALIQKW LSYPSGRFPV
     EIANEIDGTF SSSGCLNGSF LAVSNDDHYR TGTRFSGVDM NAARLLFHRL IQPDHPQISQ
     QVAASLEKNL IPKLTSSLPD VEALRFYLTL PECPLMSDCN NFTTIAIPFG TALVNLEKAP
     LKVLENWWSV LEPPLFLKIV ELFKEVVVHL LKLYKIGIPP SERRIFNSFL HTALKVLEIL
     HRVNERTGQL IQYDKFYIHE VQELIDIRND YINWVQQQAY GVDVSHGITE LADIPVTICT
     YPFVFDAQAK TTLLQTDAVL QMQMAIDQAH RQNVSSLFLP VIESVNPCLI LVVRRENIVG
     DAMEVLRKTK NIDYKKPLKV IFVGEDAVDA GGVRKEFFLL IMRELLDPKY GMFRYYEDSR
     LIWFSDKTFE DSDLFHLIGV ICGLAIYNFT IVDLHFPLAL YKKLLKRKPS LDDLKELMPD
     VGRSMQQLLD YPEDDIEETF CLNFTITVEN FGATEVKELV LNGADTAVNK QNRQEFVDAY
     VDYIFNKSVA SLFDAFHAGF HKVCGGKVLL LFQPNELQAM VIGNTNYDWK ELEKNTEYKG
     EYWAEHPTIK IFWEVFHELP LEKKKQFLLF LTGSDRIPIL GMKSLKLVIQ STGGGESYLP
     VSHTCFNLLD LPKYTEKETL RCKLIQAIDH NEGFSLI