3SI3_DENPO
ID 3SI3_DENPO Reviewed; 65 AA.
AC P25518;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Adrenergic toxin rho-elapitoxin-Dp1b {ECO:0000305};
DE Short=rho-EPTX-Dp1b {ECO:0000305};
DE AltName: Full=Muscarinic toxin CM-3;
DE AltName: Full=Protein CM-3 {ECO:0000303|PubMed:4029488};
OS Dendroaspis polylepis polylepis (Black mamba).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX NCBI_TaxID=8620;
RN [1]
RP PROTEIN SEQUENCE, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=4029488; DOI=10.1016/0020-711x(85)90367-2;
RA Joubert F.J.;
RT "The amino acid sequence of protein CM-3 from Dendroaspis polylepis
RT polylepis (black mamba) venom.";
RL Int. J. Biochem. 17:695-699(1985).
RN [2]
RP FUNCTION, AND SYNTHESIS.
RX PubMed=23648423; DOI=10.1016/j.toxicon.2013.04.017;
RA Blanchet G., Upert G., Mourier G., Gilquin B., Gilles N., Servent D.;
RT "New alpha-adrenergic property for synthetic MTbeta and CM-3 three-finger
RT fold toxins from black mamba.";
RL Toxicon 75:160-167(2013).
CC -!- FUNCTION: Highly potent on various alpha-adrenoceptors (ADRA)
CC (subnanomolar affinity for ADRA1A). Order of potency is the following:
CC ADRA1A (Ki=0.37 nM) > ADRA1B (Ki=10.47 nM) > ADRA1D (Ki=104.71 nM) >
CC ADRA2C (Ki=165.96 nM). Were also found to reversibly bind to muscarinic
CC acetylcholine receptors (CHRM), but the affinity is much weaker (CHRM1,
CC Ki=1778.28 nM; CHRM4, Ki=4466.84 nM; CHRM2, Ki=17782.79 nM).
CC {ECO:0000269|PubMed:23648423}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:4029488}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:4029488}.
CC -!- TOXIC DOSE: LD(50) is 56 mg/kg by intravenous injection.
CC {ECO:0000269|PubMed:4029488}.
CC -!- MISCELLANEOUS: Does not bind to muscarinic receptors CHRM5 and CHRM3.
CC {ECO:0000269|PubMed:23648423}.
CC -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC residue stands at position 33 (Pro-31 in standard classification).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Aminergic toxin sub-subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; P25518; -.
DR SMR; P25518; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003572; Cytotoxin_Cobra.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR PRINTS; PR00282; CYTOTOXIN.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond;
KW G-protein coupled receptor impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin.
FT CHAIN 1..65
FT /note="Adrenergic toxin rho-elapitoxin-Dp1b"
FT /evidence="ECO:0000269|PubMed:4029488"
FT /id="PRO_0000093623"
FT DISULFID 3..24
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 17..42
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 46..57
FT /evidence="ECO:0000250|UniProtKB:P60301"
FT DISULFID 58..63
FT /evidence="ECO:0000250|UniProtKB:P60301"
SQ SEQUENCE 65 AA; 7344 MW; 06EB9399CC5279F1 CRC64;
LTCVTSKSIF GITTEDCPDG QNLCFKRRHY VVPKIYDITR GCVATCPIPE NYDSIHCCKT
EKCNN
