ID   HES1B_XENLA             Reviewed;         267 AA.
AC   Q8AVU4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Transcription factor HES-1-B;
DE   AltName: Full=Hairy and enhancer of split 1-B;
GN   Name=hes1-b;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000312|EMBL:AAH41261.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Intestine {ECO:0000312|EMBL:AAI28933.1}, and
RC   Neurula {ECO:0000312|EMBL:AAH41261.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcriptional repressor of a subset of early mesodermal
CC       genes including myod1 and t/bra. Binds DNA on N-box motifs: 5'-CACNAG-
CC       3'. Acts as a negative regulator of myogenesis, mediating Notch
CC       signaling to repress expression of myod1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Transcription repression requires formation of a complex with
CC       a corepressor protein of the Groucho/TLE family. Interacts with the
CC       bHLH protein hes2, and binds DNA in the form of a heterodimer with the
CC       bHLH protein hey1/hrt1. Interacts with the bHLH protein hes6; this
CC       interaction may inhibit the transcriptional repressor activity (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q14469,
CC       ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-
CC       ProRule:PRU00981}.
CC   -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC       interrupting proline) that binds to the N-box (CACNAG), rather than the
CC       canonical E-box (CANNTG). {ECO:0000250|UniProtKB:P14003}.
CC   -!- DOMAIN: The bHLH, as well as cooperation between the central Orange
CC       domain and the C-terminal WRPW motif, is required for transcriptional
CC       repressor activity. {ECO:0000250|UniProtKB:Q6IRB2}.
CC   -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC       domain necessary for the interaction with Groucho/TLE family members,
CC       transcriptional corepressors recruited to specific target DNA by Hairy-
CC       related proteins. {ECO:0000250}.
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DR   EMBL; BC041261; AAH41261.1; -; mRNA.
DR   EMBL; BC128932; AAI28933.1; -; mRNA.
DR   RefSeq; NP_001079386.1; NM_001085917.1.
DR   AlphaFoldDB; Q8AVU4; -.
DR   SMR; Q8AVU4; -.
DR   DNASU; 379073; -.
DR   GeneID; 379073; -.
DR   KEGG; xla:379073; -.
DR   CTD; 379073; -.
DR   Xenbase; XB-GENE-6256448; hes1.S.
DR   OMA; YCGKESF; -.
DR   OrthoDB; 1427802at2759; -.
DR   Proteomes; UP000186698; Chromosome 5S.
DR   Bgee; 379073; Expressed in kidney and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0043425; F:bHLH transcription factor binding; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR   GO; GO:0048635; P:negative regulation of muscle organ development; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0007219; P:Notch signaling pathway; ISS:UniProtKB.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR003650; Orange_dom.
DR   Pfam; PF07527; Hairy_orange; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SMART; SM00511; ORANGE; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   PROSITE; PS51054; ORANGE; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..267
FT                   /note="Transcription factor HES-1-B"
FT                   /id="PRO_0000370739"
FT   DOMAIN          34..91
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   DOMAIN          110..143
FT                   /note="Orange"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           264..267
FT                   /note="WRPW motif"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        10..27
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..43
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  28663 MW;  FC9760BB6F495884 CRC64;
     MPADVMEKNS SSPVAATPAS VSNTPDKPKT ASEHRKSSKP IMEKRRRARI NESLGQLKTL
     ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRVQMTAAL STDPSVLGKY RAGFSECMNE
     VTRFLSTCEG VNTDVRTRLL GHLANCVNQI HAMNYPAQPQ IPSAAAPHPA YGQPMVQLPA
     AAPQSSPAPI ACKMGGPPVE AAKVYGGFQL VPASDGQFAF LITNPAFPQN GSVIPLYTNS
     NVGTALPPSV SPSVMPSVTA DSVWRPW