HES1_XENTR
ID HES1_XENTR Reviewed; 267 AA.
AC Q5PPM5;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Transcription factor HES-1 {ECO:0000250|UniProtKB:Q14469};
DE AltName: Full=Hairy and enhancer of split 1 {ECO:0000250|UniProtKB:Q14469};
DE AltName: Full=Protein hairy-1;
GN Name=hes1 {ECO:0000312|EMBL:AAH87608.1}; ORFNames=TNeu075p11.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000312|EMBL:AAY96799.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Qin S., Dors M., Johnson E., Bloom S., Hood L., Rowen L.;
RT "Sequence of Xenopus tropicalis development genes.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|EMBL:AAY96799.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:CAJ82991.1};
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000312|EMBL:AAY96799.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neurula {ECO:0000312|EMBL:CAJ82991.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcriptional repressor of a subset of early mesodermal
CC genes including myod1 and t/bra. Binds DNA on N-box motifs: 5'-CACNAG-
CC 3'. Acts as a negative regulator of myogenesis, mediating Notch
CC signaling to repress expression of myod1 (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein of the Groucho/TLE family. Interacts with the
CC bHLH protein hes2, and binds DNA in the form of a heterodimer with the
CC bHLH protein hey1/hrt1. Interacts with the bHLH protein hes6; this
CC interaction may inhibit the transcriptional repressor activity (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q14469,
CC ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-
CC ProRule:PRU00981}.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG). {ECO:0000250|UniProtKB:P14003}.
CC -!- DOMAIN: The bHLH, as well as cooperation between the central Orange
CC domain and the C-terminal WRPW motif, is required for transcriptional
CC repressor activity. {ECO:0000250|UniProtKB:Q6IRB2}.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho/TLE family members,
CC transcriptional corepressors recruited to specific target DNA by Hairy-
CC related proteins. {ECO:0000250}.
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DR EMBL; AC160833; AAY96799.1; -; Genomic_DNA.
DR EMBL; CR760467; CAJ82991.1; -; mRNA.
DR EMBL; BC087608; AAH87608.1; -; mRNA.
DR RefSeq; NP_001011194.1; NM_001011194.1.
DR AlphaFoldDB; Q5PPM5; -.
DR BMRB; Q5PPM5; -.
DR SMR; Q5PPM5; -.
DR STRING; 8364.ENSXETP00000062036; -.
DR PaxDb; Q5PPM5; -.
DR PRIDE; Q5PPM5; -.
DR Ensembl; ENSXETT00000036731; ENSXETP00000036731; ENSXETG00000016852.
DR GeneID; 496617; -.
DR KEGG; xtr:496617; -.
DR CTD; 3280; -.
DR Xenbase; XB-GENE-487995; hes1.
DR eggNOG; KOG4304; Eukaryota.
DR HOGENOM; CLU_068550_1_0_1; -.
DR InParanoid; Q5PPM5; -.
DR OrthoDB; 1427802at2759; -.
DR PhylomeDB; Q5PPM5; -.
DR Proteomes; UP000008143; Chromosome 5.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000016852; Expressed in neurula embryo and 13 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0048635; P:negative regulation of muscle organ development; ISS:UniProtKB.
DR GO; GO:0045665; P:negative regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007219; P:Notch signaling pathway; ISS:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..267
FT /note="Transcription factor HES-1"
FT /id="PRO_0000370740"
FT DOMAIN 34..91
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 110..143
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 264..267
FT /note="WRPW motif"
FT /evidence="ECO:0000255"
FT COMPBIAS 10..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 267 AA; 28736 MW; E36538B3DA684213 CRC64;
MPADLMEKNS SSPVAATPAS MSNTPDKPKT ASEHRKSSKP IMEKRRRARI NESLGQLKTL
ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRVQMTAAL STDPSVLGKY RAGFSECMNE
VTRFLSTCEG VNTDVRTRLL GHLANCMNQI NAMNYPTQPQ IPAAAAPHPA YGQPLVQLQG
AAPQSSPAPI ACKMGGPPVE AAKVYGGFQL VPAPDGQFAF LITNPAFPHN GSVIPVYTNS
NVGTALPPSV SPSVMPSVTA DSVWRPW
